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Human/Mouse/Rat GAPDH Antibody
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Bio-Techne Corporation
tel: (612) 379-2956
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$35 to United States
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Key Product Details
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: |
E. coli-derived recombinant human GAPDH/G3PDH Met1-Ala150 Accession # P04406 |
| Applications: | Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Polyclonal Goat IgG |
| Specificity: |
Detects human, mouse, and rat GAPDH/G3PDH in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GAPDH-2 is observed. |
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Western BlotDetection of Human/Mouse/Rat GAPDH/G3PDH by Western Blot. Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GAPDH/G3PDH at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
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ImmunocytochemistryGAPDH in HeLa Human Cell Line. GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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ImmunohistochemistryGAPDH/G3PDH in Human Kidney. GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples. |
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ImmunohistochemistryGAPDH in Human Prostate Cancer Tissue. GAPDH was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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Simple WesternDetection of Human GAPDH by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Citations for Human/Mouse/Rat GAPDH Antibody (22)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat GAPDH Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryY Basmaeil et al. (2021), Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro Cells, 10(12).
PMID: 34944000 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotTA Shah et al. (2021), Therapeutic Hypothermia Inhibits the Classical Complement Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy Frontiers in Neuroscience, 15(0):616734.
PMID: 33642979 -
Species: Hamster
Sample Types: Cell Culture Supernates
Applications: Western BlotF Pennarubia et al. (2020), Mouse WIF1 Is Only Modified with O-Fucose in Its EGF-like Domain III Despite Two Evolutionarily Conserved Consensus Sites Biomolecules, 10(9).
PMID: 32872229 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotQ Zheng et al. (2020), Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1 Cancer Cell Int, 20(0):476.
PMID: 33024414 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotT Guerenne-D et al. (2020), Multiplex coherent anti-Stokes Raman scattering microspectroscopy detection of lipid droplets in cancer cells expressing TrkB Sci Rep, 10(1):16749.
PMID: 33028922 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotJ Tan et al. (2020), scAAV2-Mediated C3 Transferase Gene Therapy in a Rat Model with Retinal Ischemia/Reperfusion Injuries Mol Ther Methods Clin Dev, 17(0):894-903.
PMID: 32382585 -
Species: Human
Sample Types: Cell
Applications: Western BlotJ Tan et al. (2020), C3 Transferase-Expressing scAAV2 Transduces Ocular Anterior Segment Tissues and Lowers Intraocular Pressure in Mouse and Monkey Mol Ther Methods Clin Dev, 17(0):143-155.
PMID: 31909087 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotA Der Vartan et al. (2019), Downregulation of POFUT1 Impairs Secondary Myogenic Fusion Through a Reduced NFATc2/IL-4 Signaling Pathway Int J Mol Sci, 20(18).
PMID: 31500188 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotMS Abdelfatta et al. (2019), Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms PLoS ONE, 14(6):e0216737.
PMID: 31194753 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotL Liu et al. (2019), PDHA1 Gene Knockout In Human Esophageal Squamous Cancer Cells Resulted In Greater Warburg Effect And Aggressive Features In Vitro And In Vivo Onco Targets Ther, 12(0):9899-9913.
PMID: 31819487 -
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western BlotT Liu et al. (2019), Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism Mucosal Immunol, 0(0).
PMID: 30664709 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotB Ilyas et al. (2018), Regulatory Evolution Drives Evasion of Host Inflammasomes by Salmonella Typhimurium Cell Rep, 25(4):825-832.e5.
PMID: 30355489 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotL Périè et al. (2017), Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice Cell. Physiol. Biochem., 44(5):1896-1911.
PMID: 29224025 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotX Li et al. (2017), Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells Sci Rep, 7(1):5081.
PMID: 28698547 -
Species: Human
Sample Types: Whole Cells
Applications: Western BlotMK Shim et al. (2017), Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis Sci Rep, 7(1):16635.
PMID: 29192289 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotR Ganesan et al. (2017), Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy PLoS Pathog, 13(2):e1006227.
PMID: 28192515 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotCT Yang et al. (2017), Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells Stem Cells, 35(4):886-897.
PMID: 28026072 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotWang L et al. (2014), Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy. Invest Ophthalmol Vis Sci, 55(7):4276-87.
PMID: 24867581 -
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western BlotChen et al. (2014), Prenatal retinoid deficiency leads to airway hyperresponsiveness in adult mice. J Clin Invest, 124(2):801-11.
PMID: 24401276 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotZhi et al. (2013), Effects of direct Renin inhibition on myocardial fibrosis and cardiac fibroblast function. PLoS ONE, 8(12):e81612.
PMID: 24349097 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotLiang D et al. (2012), The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells. BMC Cancer, 12(0):201.
PMID: 22642602 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotDungwa JV et al. (2011), Overexpression of carbonic anhydrase and HIF-1alpha in Wilms tumours. BMC Cancer, 11(0):390.
PMID: 21910893
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Customer Reviews (5)
4.8 out of 5
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Application: Simple WesternSample Tested: HT-29 human colon adenocarcinoma cell lineSpecies: HumanVerified Customer | Posted 09/28/2020
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Application: WBSample Tested: 4T1 mouse breast cancer cell line,EMT6 cellsSpecies: MouseVerified Customer | Posted 10/17/2018
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Application: WBSample Tested: HEK293 human embryonic kidney cell lineSpecies: HumanVerified Customer | Posted 07/21/2018
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Application: WBSample Tested: IPS2 induced pluripotent stem cellsSpecies: MouseVerified Customer | Posted 01/25/2018
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Application: WBSample Tested: Lysate and purified proteinSpecies: HumanVerified Customer | Posted 05/16/2017The blot contains HeLa cell lysate (1, 2, 3 and 5 µg) spiked with 200 ng of human transferrin per lane. Transferrin is detected in the red channel, tubulin is detected in the green channel and GAPDH is detected in the blue channel.
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