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Human Myocilin Antibody
Catalog # MAB3446 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 297817
Product Summary for Human Myocilin Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Myocilin
Arg33-Met504
Accession # Q99972
Arg33-Met504
Accession # Q99972
Specificity
Detects human Myocilin in direct ELISAs. In direct ELISAs, 75% cross-reactivity with recombinant mouse Myocilin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Myocilin Antibody
Myocilin in Human Skeletal Muscle.
Myocilin was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human Myocilin Monoclonal Antibody (Catalog # MAB3446) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm and sarcolemma in skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Myocilin Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Skeletal Muscle
Sample: Immersion fixed paraffin-embedded sections of Human Skeletal Muscle
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB3446 in the following applications:
Published Applications
Read 7 publications using MAB3446 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myocilin
Product Documents for Human Myocilin Antibody
Product Specific Notices for Human Myocilin Antibody
For research use only
Citations for Human Myocilin Antibody (7)
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Citations are publications that use Bio-Techne products. Selected citations for Human Myocilin Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotHF Scelsi et al. (2023-01-13), Disambiguation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability Disease Models & Mechanisms, 2023-00(0).
PMID: 36579626 -
Species: Tree Shrew
Sample Types: Whole Tissue
Applications: IHCJV Jasien et al. (2022-01-03), Anterior Segment Anatomy and Conventional Outflow Physiology of the Tree Shrew (Tupaia belangeri) Investigative Ophthalmology & Visual Science, 2022-063(1):21.
PMID: 35040876 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western BlotAC Patterson- et al. (2021-08-09), Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin The Journal of Biological Chemistry, 2021-00(0):101067.
PMID: 34384785 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCR Haribalaga et al. (2021-01-12), Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes Scientific Reports, 2021-011(1):605.
PMID: 33436790 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotSE Hill et al. (2019-07-02), Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between�benign and glaucoma-causing mutations J. Biol. Chem., 2019-00(0).
PMID: 31270212 -
Species: Human
Sample Types: Cell Lysates
Applications: ImmunoprecipitationSuntharalingam A et al. (2012-10-03), Glucose-regulated protein 94 triage of mutant myocilin through endoplasmic reticulum-associated degradation subverts a more efficient autophagic clearance mechanism. J Biol Chem, 2012-1287(48):40661-9.
PMID: 23035116 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotJoe MK et al. (2010-04-09), Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis. Am. J. Pathol., 2010-0176(6):2880-90.
PMID: 20382707
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Customer Reviews for Human Myocilin Antibody (1)
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Application: ImmunocytochemistrySample Tested: Pig TM cellsSpecies: PigVerified Customer | Posted 01/04/2017IHC showing detection of Myocilin using anti-Myocilin antibody (MAB3446) at 1:1000 dilution in Pig TM cells.
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