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Advanced Western Blotting Solutions for Cancer & Immuno-Oncology

Simple Western Advantages in Cancer Research

Simple Western™ systems provide fully automated, hands-free Western blotting analysis, giving you highly quantitative and reproducible results, and using less sample in the process.

Reproducibility & Quantitation. Simple Western eliminates the unavoidable variation of traditional Western blotting, producing intra-assay CV’s <15%. Simple Western provides quantitative and reproducible results and has a wider dynamic range than a traditional Western blot.

Limited sample. Because quantitation of protein expression via Simple Western only requires input volumes of 3 µL, the majority of your sample remains available for other characterization methods.

Sensitivity. Simple Western has chemiluminescence and industry-leading NIR/IR detection that is 100X more sensitive than the closest competitive traditional Western blot fluorescence imaging system.

Speed. Simple Western tests up to 24 samples in 3 hours and allows for the multiplex detection of multiple proteins per sample.

Simple Western Instruments - Jess & Peggy Sue Hero

Heterogeneity and single-cell resolution. Single-Cell Western on Milo™ adds single cell resolution and heterogeneity information to cell signaling studies traditionally done with conventional Western blotting and simplifies challenging phospho-flow assays done with flow cytometry.

o	Track cell differentiation by measuring edited gene and downstream markers at the single cell level with Milo

Highly Specific and Sensitive Protein Quantification

Simple Western and Single-Cell Western offer highly specific, sensitive, and reproducible quantification of important immuno-oncology targets in human cells. These targets are detected simultaneously with total protein in your sample for accurate total protein normalization.

  • Immune checkpoint proteins: PD1, PD-L1, CTLA-4, and TIGIT
  • Hypoxic markers: HIF-1α, VEGFR-2, TGF-β, and ANG2
  • CAR T-Cell Target: CD19
PDL/PDL1 3D image

Study the Tumor Microenvironment

Methodologies like flow cytometry have limitations such as off-target binding of variants or isoforms and difficulties in finding validated antibodiesSimple Western and Single-Cell Western systems partner to get you critical answers on the immune cell populations found in the tumor microenvironment, offering both population level views and single-cell level perspectives on cell subtypes.

Simple Western and Single Cell Western Graph

 

From Your Peers in Cancer Research

"[Simple Western] is easy to use, convenient, and gives answers quickly. The throughput and system automation is a definite advantage."

- Jane Gray, Ph.D., Head of Research Instrumentation, Cancer Research UK Cambridge

    Dr Jane Gray Thumbnail

     

    "I was able to screen various antibodies in a single run within half a day. This had previously taken weeks and months using traditional Western blotting methods."

    Krishna C. Yalla, Ph.D., Postdoctoral Research Scientist, Institute of Cancer Science

    Krishna C. Yalla

    High-Impact Peer-Reviewed Cancer Research

    Simple Western assays can help you reveal new insights in cancer & immuno-oncology research and advance the study of proteins, their function and signaling through reliable, quantitative, and automated workflows. Simple Western systems have been involved in breakthrough immunotherapies, including those involving immune checkpoint inhibitors, CAR T-cell therapy, bispecific antibodies, and macrophage switching. Single-Cell Westerns provide insight into population heterogeneity at the protein level and has been used to study the molecular underpinnings of malignant cell transformation with unprecedented detail.

    Cancer Cell

    Tumor Landscape Mapping of Chemo-Resistant Cancer

    In this webinar, Dr. Kyeung Min Joo, from Sungkyunkwan University School of Medicine, Seoul, South Korea describes their findings from the use of single-cell analysis technologies including Single-Cell Westerns to depict the tumor landscape of a single case of chemo-resistant metastatic, muscle-invasive urothelial bladder cancer (MIUBC).

    Immuno-oncology

    Biomarkers in Low Abundance Samples

    Simple Western requires only a few microliters of precious samples like laser capture microdissection (LCM) sample to generate multiple data points. Simple Western also has high sensitivity multiplex detection of low abundance proteins that are difficult to detect by traditional Western blot. And it’s all wrapped up in a simple workflow that minimizes your hands-on time.

    Webinar: Investigating pediatric biomarker reference intervals using new technologies

    Quantitative Cell Signaling Analysis

    In Simple Western assays, proteins are immobilized after separation, reducing variability and enabling quantitative measurements of protein abundance. High-sensitivity multiplexing is easy using multiple detection channels or RePlex to enable phospho/total measurements in the same capillary. Measure total protein in your sample for accurate total protein normalization.

    RePlex on Jess quantifies phospho and pan AKT expression with total protein normalization

    Milo Adds Single-Cell Resolution and Heterogeneity Information

    The Milo Single-Cell Western system helps detect discrete cell signaling states by measuring multiple phospho-proteins in individual cells within a population. Because cells are lysed in Single-Cell Westerns, membrane permeabilization is not required and both intracellular and intranuclear proteins are accessible for antibody probing, making it possible to use phospho-antibodies validated for Western blotting and simplifying some challenging intracellular flow assays.

    Bioprocessing Cell Heterogeneity Protein Expression with Milo

    Epigenetic Alterations in Single Nuclei

    Epigenetic alterations play important roles to drive the cancer phenotype. Use Single-Cell Westerns to investigate histone modifications in single nuclei and understand epigenetic regulation of gene expression at the single-cell level.

    Cancer histone graph

    Customer Success Stories