Unboxing the Simple Western Jess™ Instrument with CatSci
Simple Western
It didn’t take much effort at all to get the instrument connected to the computer, to get the software running…now we are ready to roll!
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Simple Western
It didn’t take much effort at all to get the instrument connected to the computer, to get the software running…now we are ready to roll!
Simple Western
With the Jess, we've been able to expand the scope of research significantly. This opens up whole new avenues and possibilities for biomarker discovery and therapeutic signalling.
Simple Western
Simple Western has and will continue to enable us to find novel synaptic targets that could potentially mitigate anxiety-like symptoms related to late-life depression and Alzheimer’s disease.
Simple Western
Jess is faster and more reliable in terms of quantification and allows us to use low sample volumes, for example, difficult-to-obtain samples such as iPSC-derived neurons.
Simple Western
Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform.
Simple Western
Simple Western™ allows us to run three-hour assays, which would usually take us two days with traditional Western blots.
Simple Western
Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease
Simple Western
The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy.
Simple Western
It can be difficult to optimize new antibodies, particularly for brain markers where you have a lot of background noise and low levels of your target. The appropriate control and low variability between plates using Simple Western™ means we can complete our studies in weeks rather than months.
Simple Western
One of the best advantages of using Wes is that it saves a lot of time, both in terms of hands-on time and in time to completion of the experiment. We were spending more than 4 hours of hands-on time per Western blot with traditional methods, and we can set up a run on the Wes in about 45 minutes. Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods.
Simple Western
We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. With Wes, we can do multiple assays with one sample collection.
Simple Western
The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. This technology has made it possible for small research labs to compete with the pace that research is conducted in large labs or companies.