
Simple Western Antibody Database
Simple Western Certified Antibodies from R&D Systems, Novus Biologicals and More!
The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. The performance of any listed antibody will need to be optimized for the biological system being examined.
You can sort by column headings or just type into the Search box.
If you don't see your antibody listed here, shoot us an email at support@proteinsimple.com.
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Simple Western Validated Antibodies
Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species▲ | Sample | Separation | Dilution | kDa on Simple Western |
---|---|---|---|---|---|---|---|---|---|
Anti-bird-IgG-HRP | Secondary |
Bethyl Laboratories A140-110P |
Anti-bird-IgG-HRP | Size | 1:250 | ||||
Anti-chicken-IgG-HRP | Secondary |
KPL 14-24-06 |
Anti-chicken-IgG-HRP | Size | 1:300 | ||||
Anti-goat-IgG-HRP | Secondary |
Invitrogen 87-9363 |
Size | 1:100-1:300 | |||||
Anti-goat-IgG-HRP | Secondary |
Thermo Fisher Scientific 31400 |
Charge | 1:100 | |||||
Anti-goat-IgG-HRP | Secondary |
Zymax 81-1620 |
Charge | 1:100-1:300 | |||||
GST-HRP | Primary |
GE RPN 1236 |
Tag | Size | 1:50-1:200 | Depends on the recombinant protein | |||
His -HRP penta | Primary |
Qiagen 34460 |
Tag | Size | 1:50-1:200 | Depends on the recombinant protein | |||
Anti-Goat IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 805-035-180 |
Anti-Goat IgG (H+L) HRP Conjugated | Bovine Polyclonal | Charge | ||||
BDNF, Biotinylated | Primary |
R&D Systems BAF248 |
BDNF, Biotinylated | Datasheet | Chicken | recombinant protein spiked into Human Serum | Size | 1:50 | 19 kDa |
TGF-B1 | Primary (biotinylated) |
R&D Systems BAF240 |
TGF-B1 | Datasheet | Chicken | recombinant protein spiked into Human Serum | Size | 1:50 | 14 |
TGF-B1 | Primary (biotinylated) |
R&D Systems BAF240 |
TGF-B1 | Datasheet | Chicken | recombinant protein spiked into Human Serum | Size | 1:50 | 14 |
Hemagglutinin 2 | Primary |
Pharma Foods International HA2#16 |
HA2 | Chicken monoclonal | Viral particles in chicken eggs | - | 1.8 µg/mL | 103 kDa | |
alpha-Fetoprotein/AFP | Primary |
R&D Systems AF1369 |
alpha-Fetoprotein/AFP | Datasheet | Chicken Polyclonal | HepG2 human hepatocellular carcinoma cell line | Size | 2 µg/mL | 70 |
Anti Sheep-HRP | Secondary |
R&D Systems HAF016 |
Datasheet | Donkey Polyclonal | Size | 1:50 | |||
Anti-Donkey IgG HRP | Secondary |
R&D Systems HAF109 |
IgG (H+L Chain) | Datasheet | Donkey Polyclonal | Detection of Goat IgG | Size | 1:50 | |
Anti-Mouse-IgG-HRP | Secondary |
R&D Systems HAF018 |
Datasheet | Donkey Polyclonal | Size | 1:100 | |||
Anti-Rabbit IgG (H+L) HRP | Secondary |
Novus Biologicals NB7185 |
Rabbit IgG (H+L) Pre-adsorbed, HRP Conjugated | Datasheet | Donkey Polyclonal | Size | 1:50 | ||
Anti-Goat IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 705-035-147 |
Anti-Goat IgG (H+L) HRP Conjugated Minimal Cross Reactivity | Donkey Polyclonal | Size | 1:100-1:300 | |||
Anti-Goat IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 705-035-003 |
Anti-Goat IgG (H+L) HRP Conjugated | Donkey Polyclonal | Charge | 1:250 | |||
Anti-Goat IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 705-035-147 |
Anti-Goat IgG (H+L) HRP Conjugated Minimal Cross Reactivity | Donkey Polyclonal | Charge | 1:100-1:300 | |||
Anti-Mouse IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 715-035-150 |
Anti-Mouse IgG (H+L) HRP Conjugated Minimal Cross Reactivity | Donkey Polyclonal | Colorectal Cancer (CRC) tissue | Charge | 1:300 | ||
Anti-Rabbit IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 711-035-152 |
Rabbit IgG (H+L) HRP Conjugated Minimal Cross Reactivity | Donkey Polyclonal | Colorectal Cancer (CRC) tissue | Charge | 1:300 | ||
Anti-Rat IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 712-035-153 |
Anti-Rat IgG (H+L) HRP Conjugated | Donkey Polyclonal | Size | 1:100-1:300 | |||
Anti-Sheep IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 713-036-147 |
F(ab')2 Fragment Donkey Anti-Sheep IgG (H+L) HRP Conjugated Minimal Cross Reactivity | Donkey Polyclonal | Size | 1:100 | |||
Anti-Sheep IgG (H+L) HRP | Secondary |
Millipore Sigma A3415 |
Anti-Sheep IgG (H+L) HRP Conjugated | Donkey Polyclonal | Size | 1:100 | |||
Donkey Anti-Mouse AlexFluor® 647 | Secondary |
Thermo Fisher Scientific A-31571 |
Anti-Mouse IgG (H+L); Jess Only | Donkey Polyclonal | - | 1:100 | |||
Donkey Anti-Rabbit AlexaFluor® 647 | Secondary |
Thermo Fisher Scientific A-31573 |
Anti-Rabbit IgG (H+L); Jess Only | Donkey Polyclonal | Size | 1:100 | |||
Donkey Anti-Rabbit HRP | Secondary |
Thermo Fisher Scientific 31458 |
Anti-Rabbit IgG (H+L) | Donkey Polyclonal | Size | 1:50 | |||
Acrp30 | Primary (biotinylated) |
R&D Systems BAF1065 |
Acrp30 | Datasheet | Goat | Human Serum | Size | 1:50 | 40 |
G-CSF | Primary (biotinylated) |
R&D Systems BAF214 |
G-CSF | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:100 | 17 |
GDF-15 | Primary (biotinylated) |
R&D Systems BAF940 |
GDF-15 | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:50 | 14 |
IFN-y | Primary (biotinylated) |
R&D Systems BAF285 |
IFN-y | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:100 | 23 |
IGF-1 | Primary (biotinylated) |
R&D Systems BAF291 |
IGF-1 | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:10 | 5 |
IL-10 | Primary |
R&D Systems AF-217-NA |
IL-10 | Datasheet | Goat | CM-PBMC + PHA | Size | 10 µg/mL | |
IL-1a | Primary (biotinylated) |
R&D Systems BAF200 |
IL-1a | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:50 | 22 |
IL-1b | Primary |
R&D Systems AF-201-NA |
IL-1b | Datasheet | Goat | THP-1 + PMA/LPS | Size | 1 µg/mL | |
IL-1b | Primary (biotinylated) |
R&D Systems BAF201 |
IL-1b | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:100 | 23 |
IL-1ra | Primary (biotinylated) |
R&D Systems BAF280 |
IL-1ra | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:300 | 23 |
IL-2 | Primary (biotinylated) |
R&D Systems BAF202 |
IL-2 | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:100 | 19 |
IL-6 | Primary |
R&D Systems AF-206-NA |
IL-6 | Datasheet | Goat | CM- Monocytes + LPS | Size | 5 µg/mL | |
IL-6 | Primary (biotinylated) |
R&D Systems BAF206 |
IL-6 | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:50 | 28 |
IL-8 | Primary (biotinylated) |
R&D Systems BAF208 |
IL-8 | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:100 | 8 |
IP-10 | Primary (biotinylated) |
R&D Systems BAF266 |
IP-10 | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:250 | 11 |
Leptin | Primary (biotinylated) |
R&D Systems BAF398 |
Leptin | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:100 | 17 |
MCP-1 | Primary (biotinylated) |
R&D Systems BAF279 |
MCP-1 | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:50 | 12 |
MMP-9 | Primary (biotinylated) |
R&D Systems BAF911 |
MMP-9 | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:300 | 146 |
TARC | Primary (biotinylated) |
R&D Systems BAF364 |
TARC | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:250 | 5 |
TARC | Primary (biotinylated) |
R&D Systems BAF364 |
TARC | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:250 | 5 |
TNF-a | Primary (biotinylated) |
R&D Systems BAF210 |
TNF-a | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:50 | 22 |
TNF-a | Primary (biotinylated) |
R&D Systems BAF210 |
TNF-a | Datasheet | Goat | recombinant protein spiked into Human Serum | Size | 1:50 | 22 |