Simple Western Antibody Database

Simple Western Certified Antibodies from R&D Systems, Novus Biologicals and More!

The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. The performance of any listed antibody will need to be optimized for the biological system being examined.

You can sort by column headings or just type into the Search box.

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Simple Western Validated Antibodies

Filter Antibodies: (3864)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
α-Nucleocapsid Primary Novus Biologicals
NB100-56683 		SARS Nucleocapsid Protein Datasheet Rabbit Polyclonal Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2) Size 25 mg/mL 60 kDa
14-3-3 γ Primary Novus Biologicals
NB100-406 		14-3-3 gamma (γ) Datasheet Mouse Monoclonal HeLa lysate 1.0 mg/ml Size 1:12.5 36 kDa
14-3-3 γ Primary Novus Biologicals
NBP2-27202 		14-3-3 gamma (γ) Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:50 33 kDa
14-3-3 γ Primary Novus Biologicals
NBP2-27370 		14-3-3 gamma (γ) Datasheet Rabbit Polyclonal Human Brain lysate 0.5 mg/ml Size 1:10 34 kDa
14-3-3 ζ Primary R&D Systems
MAB2669 		14-3-3 zeta (ζ) Datasheet Mouse Monoclonal SH-SY5Y human neuroblastoma cell line Size 2 µg/mL 34 kDa
15-PGDH/HPGD Primary Novus Biologicals
NB200-179 		15-PGDH/HPGD Datasheet Rabbit polyclonal Lovo (left) and HeLa (right) lysate 0.5 mg/ml Size 1:500
15-PGDH/HPGD Primary R&D Systems
AF5660 		15-PGDH/HPGD Datasheet Goat Polyclonal Human liver tissue Size 10 µg/mL 34 kDa
17 beta-HSD1/HSD17B1 Primary R&D Systems
MAB7178 		17 beta-HSD1/HSD17B1 Datasheet Mouse Monoclonal Human placenta tissue Size 2.5 µg/mL 40 kDa
4-Hydroxynonenal Primary R&D Systems
MAB3249 		4-Hydroxynonenal Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) Size 5 µg/mL
4EBP1 Primary R&D Systems
AF3227 		4EBP1 Datasheet Goat Polyclonal HeLa human cervical epithelial carcinoma cell line Size 5 µg/mL 26 kDa
5'-Nucleotidase/CD73 Primary R&D Systems
AF5795 		5'-Nucleotidase/CD73 Datasheet Sheep Polyclonal U266 human myeloma cell line Size 10 µg/mL 86
5-HT7 Primary Novus Biologicals
NB100-56352 		5-Hydroxytryptamine receptor 7 Datasheet Rabbit Polyclonal Rat Brain Lysate Size 20 µg/mL 59 kDa
5-Lipoxygenase Primary Novus Biologicals
NB110-58748 		5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 50 µg/mL
5-Lipoxygenase Primary Novus Biologicals
NB110-58749 		5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 10 µg/mL
5HT1D Primary Novus Biologicals
NB100-56349 		5HT1D Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:10
5HT3A Primary Novus Biologicals
NB100-56351 		5HT3A Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:50 65
6-His Epitope Tag Primary Novus Biologicals
NB100-64768 		6-His Epitope Tag Datasheet Mouse monoclonal rhEDG6 CHO S Tx lysate 0.1 mg/ml Size 1:250
A. aeolicus BPL/BioID2 Primary Novus Biologicals
NBP2-59940 		A. aeolicus BPL/BioID2 Antibody (SS QD1) Datasheet Mouse Monoclonal Size 94 kDa
A20/TNFAIP3 Primary Novus Biologicals
NBP1-77533 		A20/TNFAIP3 Datasheet Mouse monoclonal Jurkat lysate 0.05 mg/ml Size 1:100 102
A2BP1 Primary Novus Biologicals
NBP2-13169 		A2BP1 Datasheet Mouse monoclonal Human Brain lysate 0.5 mg/ml Size 1:20 54
ABCA1 Primary Novus Biologicals
NB400-105 		ABCA1 Datasheet Rabbit polyclonal Charge 1:50
ABCF2 Primary Novus Biologicals
NB400-115 		ABCF2 Datasheet Rabbit polyclonal HeLa lysate 0.05 mg/ml Size 1:1000 77
ABCG2 Primary Novus Biologicals
NBP1-59749 		ABCG2 Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 72
Abhd5 Primary Novus Biologicals
NB110-41576 		Abhd5 Datasheet Rabbit polyclonal MCF-7 lysate 0.5 mg/ml Size 1:1000 55
ACADL Primary Novus Biologicals
NBP1-54936 		ACADL Antibody Datasheet Rabbit Polyclonal Rat/mouse liver Size 1:50 48 kDa
ACE-2 Primary R&D Systems
AF3437 		ACE-2 Datasheet Goat Polyclonal Mouse kidney tissue Size 2.5 µg/mL 141
ACE-2 Primary R&D Systems
AF933 		ACE-2 Datasheet Goat Polyclonal Human kidney tissue Size 10 µg/mL 155
ACE-2 Primary R&D Systems
AF933 		ACE-2 Datasheet Goat Polyclonal Recombinant Human ACE-2 Protein, CF (R&D Systems PN 933-ZN-010) Size 1:50 158
ACE/CD143 Primary R&D Systems
AF1513 		ACE/CD143 Datasheet Goat Polyclonal Mouse lung tissue Size 10 µg/mL 218
Acrp30 Primary (biotinylated) R&D Systems
BAF1065 		Acrp30 Datasheet Goat Human Serum Size 1:50 40
ACSF2 Primary Novus Biologicals
NBP2-47558 		ACSF2 Antibody Datasheet Rabbit Polyclonal Size
ACSL1 Primary Novus Biologicals
NB110-99585 		ACSL1 Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:100 65
ACSL3 Primary Novus Biologicals
NBP2-15252 		ACSL3 Antibody Datasheet Rabbit Polyclonal Mouse brain (hippocampus) tissue Size
ACSL5 Primary Novus Biologicals
NBP1-59645 		ACSL5 Antibody Datasheet Rabbit Polyclonal Mouse liver lysate Size
Actin Primary Novus Biologicals
NB100-74340 		Actin Datasheet Mouse monoclonal HeLa lysate 0.1 mg/ml Size 1:25 50
Actin Gamma 1 Primary Novus Biologicals
NB600-533 		Actin Gamma 1 Datasheet Rabbit polyclonal HeLa lysate 1.0 mg/ml Size 1:100 49
ADAMTS13 Primary R&D Systems
AF4245 		Datasheet Sheep Polyclonal Size 1:50-1:300 129, 217
ADARB1 Primary Novus Biologicals
NBP1-90240 		ADARB1 Datasheet Rabbit Polyclonal RT-4, U-251MG Size 1:20 94
ADE2 Primary Novus Biologicals
NBP1-90235 		ADE2 Datasheet Rabbit Polyclonal RT-4 and U-251MG Size 1:20 47/50
Adenosine A2a R Primary Novus Biologicals
NB300-597 		Adenosine A2a R Datasheet Rabbit polyclonal HeLa lysate 0.5 mg/ml Size 1:20 51
ADFP Primary Novus Biologicals
NB110-40877 		ADFP Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 77
ADFP Primary Novus Biologicals
NB110-40878 		ADFP Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 46
ADI1 Primary Novus Biologicals
NBP2-38251 		ADI1 Datasheet Rabbit Polyclonal HepG2 lysate Size 1:25 26
Adiponectin/Acrp30 Primary R&D Systems
AF1065 		Adiponectin/Acrp30 Datasheet Goat Polyclonal Human placenta tissue Size 10 µg/mL 41
AGR2 Primary Novus Biologicals
NBP2-27392 		AGR2 Datasheet Rabbit polyclonal MCF-7 lysate 0.5 mg/ml Size 1:100 27
AGR2 Primary Novus Biologicals
NBP2-27393 		AGR2 Datasheet Rabbit polyclonal MCF-7 lysate 0.5 mg/ml Size 1:50 22
AGXT Primary Novus Biologicals
NBP1-89200 		AGXT Datasheet Rabbit Polyclonal Liver (left), HepG2 (right) Size 1:20 47
AHR Primary R&D Systems
AF6185 		AHR Datasheet Sheep Polyclonal PC-3 human prostate cancer cell line Size 10 µg/mL 109
AIF Primary Novus Biologicals
NBP2-27177 		AIF Datasheet Rabbit polyclonal Jurkat and MCF-7 lysate 0.2 mg/ml Size 1:500
AIF Primary R&D Systems
AF1457 		AIF Datasheet Rabbit Polyclonal Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line Size 1 µg/mL 69
AIF Primary R&D Systems
AF5824 		AIF Datasheet Sheep Polyclonal Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line Size 10 µg/mL 69
Aiolos/IKZF3 Primary Novus Biologicals
NBP2-16938 		Aiolos/IKZF3 Datasheet Rabbit Polyclonal RPMI 8266 cell line 0.1 mg/mL Size 1:50 35-77
AIP/ARA9 Primary Novus Biologicals
NB100-127 		AIP/ARA9 Datasheet Mouse monoclonal Hek293 lysate 0.5 mg/ml Size 1:200 44
AKT Primary R&D Systems
MAB2055 		Akt Pan Specific Datasheet Mouse Monoclonal MCF-7 human breast cancer cell line Size 2 µg/mL 62
Akt Primary R&D Systems
MAB2055 		Unmodified Citations (1)
Datasheet 		Mouse Monoclonal A-549 Size Not_Stated Not_Stated
Akt Primary R&D Systems
MAB2055 		Unmodified Citations (1)
Datasheet 		Mouse Monoclonal A-549 Size Not_Stated Not_Stated
AKT, phospho Primary R&D Systems
AF887 		pAKT (Ser473) Datasheet Rabbit Polyclonal MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line treated with Recombinant Human IGF-I Size 5 µg/mL 60
AKT, phospho Primary R&D Systems
MAB887 		pAKT (Ser473) Datasheet Mouse Monoclonal MCF-7 human breast cancer cell line treated with Recombinant Human IGF-I Size 2 µg/mL 65
AKT1 Primary R&D Systems
AF1775 		AKT1 Datasheet Rabbit polyclonal HeLa human cervical epithelial carcinoma cell line Size 1 µg/mL 63
Akt2 Primary R&D Systems
AF2315 		Akt2 Datasheet Rabbit Polyclonal MCF-7 human breast cancer cell line Size 2.5 µg/mL 60
Akt2 Primary R&D Systems
AF23151 		Akt2 Datasheet Goat Polyclonal MCF-7 human breast cancer cell line Size 5 µg/mL 60
ALAD Primary Novus Biologicals
NBP1-89158 		ALAD Datasheet Rabbit Polyclonal h. Liver (left) and h. Adrenal (right) lysates Size 1:200 39
ALAS1 Primary Novus Biologicals
NB100-56415 		ALAS1 Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 65
Albumin Primary R&D Systems
MAB1455 		Albumin Datasheet Mouse Monoclonal Human liver tissue Size 10 µg/mL 64 kDa
Albumin Primary R&D Systems
AF3329 		Albumin Datasheet Goat Polyclonal Human liver tissue, mouse liver tissue, hepatocyte differentiated iBJ6 human induced pluripotent stem cell line, and human serum Size 5-10 µg/mL 64 kDa
ALCAM/CD166 Primary R&D Systems
AF1172 		ALCAM/CD166 Datasheet Goat Polyclonal Mouse brain (total) tissue and mouse lung tissue Size 12.5 µg/mL 149
alcohol dehydrogenase 5 Primary Novus Biologicals
NBP2-49350 		alcohol dehydrogenase 5 Antibody Datasheet Rabbit Polyclonal Size
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary R&D Systems
AF5869 		Aldehyde Dehydrogenase 1-A1/ALDH1A1 Datasheet Goat Polyclonal Human liver tissue and mouse liver tissue Size 10 µg/mL 57 kDa
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary R&D Systems
MAB5869 		Aldehyde Dehydrogenase 1-A1/ALDH1A1 Datasheet Mouse Monoclonal Human liver tissue Size 20 µg/mL 58 kDa
ALDH1A2 Primary Novus Biologicals
NBP1-87158 		ALDH1A2 Antibody Datasheet Rabbit Polyclonal Wild Type K562 cells, Imatinib Resistant K562 cells, Nilotinib Resistant K562 cells and Dasatinib Resistant K56 cells Size 1:50
ALDH2 Primary R&D Systems
MAB10168 		ALDH2 Antibody (1007902) [Unconjugated] Datasheet Mouse Monoclonal Human liver tissue, Mouse liver tissue, and Rat liver tissue 0.2 mg/mL Size 20 µg/mL 55 kDa
Aldo-keto Reductase 1C1/AKR1C1 Primary R&D Systems
MAB6529 		Aldo-keto Reductase 1C1/AKR1C1 Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line and human liver tissue Size 1 µg/mL 41 kDa
Aldo-keto Reductase 1C3/AKR1C3 Primary R&D Systems
MAB7678 		Aldo-keto Reductase 1C3/AKR1C3 Datasheet Mouse Monoclonal A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line Size 5 µg/mL 45 kDa
Alix Primary Novus Biologicals
NBP1-49701 		Alix Datasheet Rabbit Polyclonal Human Plasma (Novus Biologicals PN NBP2-49838), Human Serum (Novus Biologicals NBP2-49827), and Human A549 Cells (Novus Biologicals PN NBP2-49862) 0.2 mg/mL Size 1:10 109
ALK-1 Primary R&D Systems
MAB370 		ALK-1 Datasheet Mouse Monoclonal Human placenta tissue and human lung tissue Size 40 µg/mL 63
Alkaline Phosphatase/ALPP Primary R&D Systems
MAB59051 		Alkaline Phosphatase/ALPP Datasheet Mouse Monoclonal JAR human choriocarcinoma cell line Size 10 µg/mL 74 kDa
Alkaline Phosphatase/ALPP/ALPI Primary R&D Systems
AF5905 		Alkaline Phosphatase/ALPP/ALPI Datasheet Sheep Polyclonal Human placenta tissue Size 10 µg/mL 76
alpha 1-Acid Glycoprotein Primary R&D Systems
AF3694 		alpha 1-Acid Glycoprotein Datasheet Goat Polyclonal Human serum Size 5 µg/mL 79 kda
Alpha 1-Microglobulin Primary R&D Systems
MAB7720 		alpha 1-Microglobulin Datasheet Mouse monoclonal Rat plasma Size 20 µg/mL 37
alpha 1B-Glycoprotein Primary R&D Systems
AF7757 		alpha 1B-Glycoprotein Datasheet Sheep Polyclonal Human liver tissue Size 10 µg/mL 94 kDa
alpha 1B-Glycoprotein Primary R&D Systems
AF8537 		alpha 1B-Glycoprotein Datasheet Rabbit Polyclonal Mouse plasma and rat plasma Size 1:100 113
alpha 2-Macroglobulin Primary R&D Systems
AF1938 		alpha 2-Macroglobulin Datasheet Goat Polyclonal Human serum Size 1 µg/mL 178
alpha 2-Macroglobulin Primary R&D Systems
MAB1938 		alpha 2-Macroglobulin Datasheet Mouse Monoclonal Human serum Size 10 µg/mL 178
Alpha Synuclein Primary Novus Biologicals
NBP1-05194 		Alpha Synuclein Datasheet Mouse monoclonal Human Brain lysate 0.05 mg/ml Size 1:2000 20
Alpha tubulin Primary Novus Biologicals
NB100-690 		Alpha tubulin Datasheet Mouse monoclonal HeLa lysate 1.0 mg/ml Size 1:50 55
alpha Tubulin 4a Primary Novus Biologicals
NB120-11304 		alpha Tubulin 4a Antibody (B-5-1-2) Datasheet Mouse Monoclonal Caco2 cell lysates Size 1:50
alpha-Actinin 1 Primary R&D Systems
MAB8279 		alpha-Actinin 1 Datasheet Mouse Monoclonal C2C12 mouse myoblast cell line Size 2 µg/mL 98
alpha-Actinin 1 Primary R&D Systems
MAB8279 		Unmodified Citations (1)
Datasheet 		Mouse Monoclonal Caco-2/TC-7 Size Not_Stated Not_Stated
alpha-Actinin 1 Primary R&D Systems
MAB8279 		Unmodified Citations (1)
Datasheet 		Mouse Monoclonal Caco-2/TC-7 Size Not_Stated Not_Stated
alpha-Fetoprotein/AFP Primary Novus Biologicals
NB110-7961 		alpha-Fetoprotein/AFP Datasheet Mouse Monoclonal Size
alpha-Fetoprotein/AFP Primary R&D Systems
AF1369 		alpha-Fetoprotein/AFP Datasheet Chicken Polyclonal HepG2 human hepatocellular carcinoma cell line Size 2 µg/mL 70
Alpha-Fetoprotein/AFP Primary R&D Systems
MAB1368 		Alpha-Fetoprotein Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line Size 10 µg/mL 70
alpha-Fetoprotein/AFP Primary R&D Systems
MAB13692 		alpha-Fetoprotein/AFP Antibody (189508) Datasheet Mouse Monoclonal HepG2 and HepB3 lysate 0.2 mg/ml Size 5 µg/mL 67 kDa (HepB3), 68 kDa (HepG2)
alpha-Galactosidase A/GLA Primary R&D Systems
AF6146 		alpha-Galactosidase A/GLA Antibody [Unconjugated] Datasheet Sheep Polyclonal MCF-7 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line 0.2 mg/mL Size 50 µg/mL 55 kDa
alpha-Internexin Primary Novus Biologicals
NB300-140 		alpha-Internexin Datasheet Mouse Monoclonal SH-SY5Y lysate Size 1:200 140
alpha-Smooth Muscle Actin Primary R&D Systems
MAB1420 		alpha-Smooth Muscle Actin Datasheet Mouse Monoclonal iBJ6 human induced pluripotent stem cell line differentiated to cardiomyocytes Size 10 µg/mL 49
alpha-Smooth Muscle Actin Primary Novus Biologicals
NBP2-33006 		alpha-Smooth Muscle Actin Datasheet Mouse Monoclonal h. Aorta lysate Size 10 µg/mL 48-50
alpha-Smooth Muscle Actin Primary Novus Biologicals
NBP2-33006 		alpha-Smooth Muscle Actin Datasheet Mouse Monoclonal h. Uterus lysate Size 10 µg/mL 48-50
AlphaA Crystallin/CRYAA Primary R&D Systems
AF4848 		AlphaA Crystallin/CRYAA Datasheet Goat Polyclonal Mouse eye lens tissue Size 1 µg/mL 28 kDa
AlphaB Crystallin/CRYAB Primary R&D Systems
AF4849 		AlphaB Crystallin/CRYAB Datasheet Goat Polyclonal Human heart tissue and mouse heart tissue Size 2 µg/mL 29 kDa
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