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Simple Western Antibody Database

Simple Western Certified Antibodies from R&D Systems, Novus Biologicals and More!

The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. The performance of any listed antibody will need to be optimized for the biological system being examined.

You can sort by column headings or just type into the Search box.

If you don't see your antibody listed here, shoot us an email at support@proteinsimple.com.

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Simple Western Validated Antibodies


Filter Antibodies: (3016)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
α-Nucleocapsid Primary Novus Biologicals
NB100-56683
SARS Nucleocapsid Protein Datasheet Rabbit Polyclonal Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2) Size 25 mg/mL 60 kDa
14-3-3 γ Primary Novus Biologicals
NBP2-27202
14-3-3 gamma (γ) Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:50 33 kDa
14-3-3 γ Primary Novus Biologicals
NBP2-27370
14-3-3 gamma (γ) Datasheet Rabbit Polyclonal Human Brain lysate 0.5 mg/ml Size 1:10 34 kDa
14-3-3 γ Primary Novus Biologicals
NB100-406
14-3-3 gamma (γ) Datasheet Mouse Monoclonal HeLa lysate 1.0 mg/ml Size 1:12.5 36 kDa
14-3-3 ζ Primary R&D Systems
MAB2669
14-3-3 zeta (ζ) Datasheet Mouse Monoclonal SH-SY5Y human neuroblastoma cell line Size 2 µg/mL 34 kDa
15-PGDH/HPGD Primary Novus Biologicals
NB200-179
15-PGDH/HPGD Datasheet Rabbit polyclonal Lovo (left) and HeLa (right) lysate 0.5 mg/ml Size 1:500
15-PGDH/HPGD Primary R&D Systems
AF5660
15-PGDH/HPGD Datasheet Goat Polyclonal Human liver tissue Size 10 µg/mL 34 kDa
17 beta-HSD1/HSD17B1 Primary R&D Systems
MAB7178
17 beta-HSD1/HSD17B1 Datasheet Mouse Monoclonal Human placenta tissue Size 2.5 µg/mL 40 kDa
4-Hydroxynonenal Primary R&D Systems
MAB3249
4-Hydroxynonenal Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) Size 5 µg/mL
4EBP1 Primary R&D Systems
AF3227
4EBP1 Datasheet Goat Polyclonal HeLa human cervical epithelial carcinoma cell line Size 5 µg/mL 26 kDa
5'-Nucleotidase/CD73 Primary R&D Systems
AF5795
5'-Nucleotidase/CD73 Datasheet Sheep Polyclonal U266 human myeloma cell line Size 10 µg/mL 86
5-HT7 Primary Novus Biologicals
NB100-56352
5-Hydroxytryptamine receptor 7 Rabbit Polyclonal Rat Brain Lysate Size 20 µg/mL 59 kDa
5-Lipoxygenase Primary Novus Biologicals
NB110-58748
5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 50 µg/mL
5-Lipoxygenase Primary Novus Biologicals
NB110-58749
5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 10 µg/mL
5HT1D Primary Novus Biologicals
NB100-56349
5HT1D Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:10
5HT3A Primary Novus Biologicals
NB100-56351
5HT3A Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:50 65
6-His Epitope Tag Primary Novus Biologicals
NB100-64768
6-His Epitope Tag Datasheet Mouse monoclonal rhEDG6 CHO S Tx lysate 0.1 mg/ml Size 1:250
A. aeolicus BPL/BioID2 Primary Novus Biologicals
NBP2-59940
A. aeolicus BPL/BioID2 Antibody (SS QD1) Mouse Monoclonal Size 94 kDa
A. aeolicus BPL/BioID2 Primary Novus Biologicals
NBP2-59940
A. aeolicus BPL/BioID2 Antibody (SS QD1) Mouse Monoclonal Size 94 kDa
A20/TNFAIP3 Primary Novus Biologicals
NBP1-77533
A20/TNFAIP3 Datasheet Mouse monoclonal Jurkat lysate 0.05 mg/ml Size 1:100 102
A2BP1 Primary Novus Biologicals
NBP2-13169
A2BP1 Datasheet Mouse monoclonal Human Brain lysate 0.5 mg/ml Size 1:20 54
ABCA1 Primary Novus Biologicals
NB400-105
ABCA1 Rabbit polyclonal Charge 1:50
ABCF2 Primary Novus Biologicals
NB400-115
ABCF2 Datasheet Rabbit polyclonal HeLa lysate 0.05 mg/ml Size 1:1000 77
ABCG2 Primary Novus Biologicals
NBP1-59749
ABCG2 Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 72
Abhd5 Primary Novus Biologicals
NB110-41576
Abhd5 Datasheet Rabbit polyclonal MCF-7 lysate 0.5 mg/ml Size 1:1000 55
ACADL Primary Novus Biologicals
NBP1-54936
ACADL Antibody Rabbit Polyclonal Rat/mouse liver Size 1:50 48 kDa
ACE-2 Primary R&D Systems
AF3437
ACE-2 Datasheet Goat Polyclonal Mouse kidney tissue Size 2.5 µg/mL 141
ACE-2 Primary R&D Systems
AF933
ACE-2 Datasheet Goat Polyclonal Recombinant Human ACE-2 Protein, CF (R&D Systems PN 933-ZN-010) Size 1:50 158
ACE-2 Primary R&D Systems
AF933
ACE-2 Datasheet Goat Polyclonal Recombinant Human ACE-2 Protein, CF (R&D Systems PN 933-ZN-010) Size 1:50 158
ACE-2 Primary R&D Systems
AF933
ACE-2 Datasheet Goat Polyclonal Human kidney tissue Size 10 µg/mL 155
ACE/CD143 Primary R&D Systems
AF1513
ACE/CD143 Datasheet Goat Polyclonal Mouse lung tissue Size 10 µg/mL 218
Acrp30 Primary (biotinylated) R&D Systems
BAF1065
Acrp30 Goat Human Serum Size 1:50 40
ACSF2 Primary Novus Biologicals
NBP2-47558
ACSF2 Antibody Rabbit Polyclonal Size
ACSF2 Primary Novus Biologicals
NBP2-47558
ACSF2 Antibody Rabbit Polyclonal Size
ACSL1 Primary Novus Biologicals
NB110-99585
ACSL1 Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:100 65
ACSL3 Primary Novus Biologicals
NBP2-15252
ACSL3 Antibody Rabbit Polyclonal Mouse brain (hippocampus) tissue Size
ACSL3 Primary Novus Biologicals
NBP2-15252
ACSL3 Antibody Rabbit Polyclonal Mouse brain (hippocampus) tissue Size
ACSL5 Primary Novus Biologicals
NBP1-59645
ACSL5 Antibody Rabbit Polyclonal Mouse liver lysate Size
ACSL5 Primary Novus Biologicals
NBP1-59645
ACSL5 Antibody Rabbit Polyclonal Mouse liver lysate Size
Actin Primary Novus Biologicals
NB100-74340
Actin Datasheet Mouse monoclonal HeLa lysate 0.1 mg/ml Size 1:25 50
Actin Gamma 1 Primary Novus Biologicals
NB600-533
Actin Gamma 1 Datasheet Rabbit polyclonal HeLa lysate 1.0 mg/ml Size 1:100 49
ADAMTS13 Primary R&D Systems
AF4245
Sheep Polyclonal Size 1:50-1:300 129, 217
ADARB1 Primary Novus Biologicals
NBP1-90240
ADARB1 Rabbit Polyclonal RT-4, U-251MG Size 1:20 94
ADE2 Primary Novus Biologicals
NBP1-90235
ADE2 Rabbit Polyclonal RT-4 and U-251MG Size 1:20 47/50
Adenosine A2a R Primary Novus Biologicals
NB300-597
Adenosine A2a R Datasheet Rabbit polyclonal HeLa lysate 0.5 mg/ml Size 1:20 51
ADFP Primary Novus Biologicals
NB110-40877
ADFP Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 77
ADFP Primary Novus Biologicals
NB110-40878
ADFP Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 46
ADI1 Primary Novus Biologicals
NBP2-38251
ADI1 Rabbit Polyclonal HepG2 lysate Size 1:25 26
Adiponectin/Acrp30 Primary R&D Systems
AF1065
Adiponectin/Acrp30 Datasheet Goat Polyclonal Human placenta tissue Size 10 µg/mL 41
AGR2 Primary Novus Biologicals
NBP2-27393
AGR2 Datasheet Rabbit polyclonal MCF-7 lysate 0.5 mg/ml Size 1:50 22
AGR2 Primary Novus Biologicals
NBP2-27392
AGR2 Datasheet Rabbit polyclonal MCF-7 lysate 0.5 mg/ml Size 1:100 27
AGXT Primary Novus Biologicals
NBP1-89200
AGXT Rabbit Polyclonal Liver (left), HepG2 (right) Size 1:20 47
AHR Primary R&D Systems
AF6185
AHR Datasheet Sheep Polyclonal PC-3 human prostate cancer cell line Size 10 µg/mL 109
AIF Primary Novus Biologicals
NBP2-27177
AIF Datasheet Rabbit polyclonal Jurkat and MCF-7 lysate 0.2 mg/ml Size 1:500
AIF Primary R&D Systems
AF1457
AIF Datasheet Rabbit Polyclonal Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line Size 1 µg/mL 69
AIF Primary R&D Systems
AF5824
AIF Datasheet Sheep Polyclonal Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line Size 10 µg/mL 69
Aiolos/IKZF3 Primary Novus Biologicals
NBP2-16938
Aiolos/IKZF3 Rabbit Polyclonal RPMI 8266 cell line 0.1 mg/mL Size 1:50 35-77
Aiolos/IKZF3 Primary Novus Biologicals
NBP2-16938
Aiolos/IKZF3 Rabbit Polyclonal RPMI 8266 cell line 0.1 mg/mL Size 1:50 35-77
AIP/ARA9 Primary Novus Biologicals
NB100-127
AIP/ARA9 Datasheet Mouse monoclonal Hek293 lysate 0.5 mg/ml Size 1:200 44
AKT Primary R&D Systems
MAB2055
Akt Pan Specific Datasheet Mouse Monoclonal MCF-7 human breast cancer cell line Size 2 µg/mL 62
AKT, phospho Primary R&D Systems
MAB887
pAKT (Ser473) Datasheet Mouse Monoclonal MCF-7 human breast cancer cell line treated with Recombinant Human IGF-I Size 2 µg/mL 65
AKT, phospho Primary R&D Systems
AF887
pAKT (Ser473) Datasheet Rabbit Polyclonal MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line treated with Recombinant Human IGF-I Size 5 µg/mL 60
AKT1 Primary R&D Systems
AF1775
AKT1 Datasheet Rabbit polyclonal HeLa human cervical epithelial carcinoma cell line Size 1 µg/mL 63
Akt2 Primary R&D Systems
AF2315
Akt2 Datasheet Rabbit Polyclonal MCF-7 human breast cancer cell line Size 2.5 µg/mL 60
Akt2 Primary R&D Systems
AF23151
Akt2 Datasheet Goat Polyclonal MCF-7 human breast cancer cell line Size 5 µg/mL 60
ALAD Primary Novus Biologicals
NBP1-89158
ALAD Rabbit Polyclonal h. Liver (left) and h. Adrenal (right) lysates Size 1:200 39
ALAS1 Primary Novus Biologicals
NB100-56415
ALAS1 Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 65
Albumin Primary R&D Systems
MAB1455
Albumin Mouse Monoclonal Human liver tissue Size 10 µg/mL 64 kDa
Albumin Primary R&D Systems
AF3329
Albumin Datasheet Goat Polyclonal Human liver tissue, mouse liver tissue, hepatocyte differentiated iBJ6 human induced pluripotent stem cell line, and human serum Size 5-10 µg/mL 64 kDa
ALCAM/CD166 Primary R&D Systems
AF1172
ALCAM/CD166 Datasheet Goat Polyclonal Mouse brain (total) tissue and mouse lung tissue Size 12.5 µg/mL 149
alcohol dehydrogenase 5 Primary Novus Biologicals
NBP2-49350
alcohol dehydrogenase 5 Antibody Rabbit Polyclonal Size
alcohol dehydrogenase 5 Primary Novus Biologicals
NBP2-49350
alcohol dehydrogenase 5 Antibody Rabbit Polyclonal Size
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary R&D Systems
AF5869
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Datasheet Goat Polyclonal Human liver tissue and mouse liver tissue Size 10 µg/mL 57 kDa
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary R&D Systems
MAB5869
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Datasheet Mouse Monoclonal Human liver tissue Size 20 µg/mL 58 kDa
ALDH1A2 Primary Novus Biologicals
NBP1-87158
ALDH1A2 Antibody Rabbit Polyclonal Wild Type K562 cells, Imatinib Resistant K562 cells, Nilotinib Resistant K562 cells and Dasatinib Resistant K56 cells Size 1:50
ALDH1A2 Primary Novus Biologicals
NBP1-87158
ALDH1A2 Antibody Rabbit Polyclonal Wild Type K562 cells, Imatinib Resistant K562 cells, Nilotinib Resistant K562 cells and Dasatinib Resistant K56 cells Size 1:50
ALDH2 Primary R&D Systems
MAB10168
ALDH2 Antibody (1007902) [Unconjugated] Mouse Monoclonal Human liver tissue, Mouse liver tissue, and Rat liver tissue 0.2 mg/mL Size 20 µg/mL 55 kDa
Aldo-keto Reductase 1C1/AKR1C1 Primary R&D Systems
MAB6529
Aldo-keto Reductase 1C1/AKR1C1 Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line and human liver tissue Size 1 µg/mL 41 kDa
Aldo-keto Reductase 1C3/AKR1C3 Primary R&D Systems
MAB7678
Aldo-keto Reductase 1C3/AKR1C3 Datasheet Mouse Monoclonal A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line Size 5 µg/mL 45 kDa
Alix Primary Novus Biologicals
NBP1-49701
Alix Rabbit Polyclonal Human Plasma (Novus Biologicals PN NBP2-49838), Human Serum (Novus Biologicals NBP2-49827), and Human A549 Cells (Novus Biologicals PN NBP2-49862) 0.2 mg/mL Size 1:10 109
ALK-1 Primary R&D Systems
MAB370
ALK-1 Datasheet Mouse Monoclonal Human placenta tissue and human lung tissue Size 40 µg/mL 63
Alkaline Phosphatase/ALPP Primary R&D Systems
MAB59051
Alkaline Phosphatase/ALPP Datasheet Mouse Monoclonal JAR human choriocarcinoma cell line Size 10 µg/mL 74 kDa
Alkaline Phosphatase/ALPP/ALPI Primary R&D Systems
AF5905
Alkaline Phosphatase/ALPP/ALPI Datasheet Sheep Polyclonal Human placenta tissue Size 10 µg/mL 76
alpha 1-Acid Glycoprotein Primary R&D Systems
AF3694
alpha 1-Acid Glycoprotein Datasheet Goat Polyclonal Human serum Size 5 µg/mL 79 kda
Alpha 1-Microglobulin Primary R&D Systems
MAB7720
alpha 1-Microglobulin Datasheet Mouse monoclonal Rat plasma Size 20 µg/mL 37
alpha 1B-Glycoprotein Primary R&D Systems
AF8537
alpha 1B-Glycoprotein Datasheet Rabbit Polyclonal Mouse plasma and rat plasma Size 1:100 113
alpha 1B-Glycoprotein Primary R&D Systems
AF7757
alpha 1B-Glycoprotein Datasheet Sheep Polyclonal Human liver tissue Size 10 µg/mL 94 kDa
alpha 2-Macroglobulin Primary R&D Systems
AF1938
alpha 2-Macroglobulin Datasheet Goat Polyclonal Human serum Size 1 µg/mL 178
alpha 2-Macroglobulin Primary R&D Systems
MAB1938
alpha 2-Macroglobulin Datasheet Mouse Monoclonal Human serum Size 10 µg/mL 178
Alpha Synuclein Primary Novus Biologicals
NBP1-05194
Alpha Synuclein Datasheet Mouse monoclonal Human Brain lysate 0.05 mg/ml Size 1:2000 20
Alpha tubulin Primary Novus Biologicals
NB100-690
Alpha tubulin Datasheet Mouse monoclonal HeLa lysate 1.0 mg/ml Size 1:50 55
alpha Tubulin 4a Primary Novus Biologicals
NB120-11304
alpha Tubulin 4a Antibody (B-5-1-2) Mouse Monoclonal Caco2 cell lysates Size 1:50
alpha-Actinin 1 Primary R&D Systems
MAB8279
alpha-Actinin 1 Datasheet Mouse Monoclonal C2C12 mouse myoblast cell line Size 2 µg/mL 98
Alpha-Fetoprotein/AFP Primary R&D Systems
MAB1368
Alpha-Fetoprotein Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line Size 10 µg/mL 70
alpha-Fetoprotein/AFP Primary R&D Systems
AF1369
alpha-Fetoprotein/AFP Datasheet Chicken Polyclonal HepG2 human hepatocellular carcinoma cell line Size 2 µg/mL 70
alpha-Fetoprotein/AFP Primary Novus Biologicals
NB110-7961
alpha-Fetoprotein/AFP Mouse Monoclonal Size
alpha-Fetoprotein/AFP Primary R&D Systems
MAB13692
alpha-Fetoprotein/AFP Antibody (189508) Mouse Monoclonal HepG2 and HepB3 lysate 0.2 mg/ml Size 5 µg/mL 67 kDa (HepB3), 68 kDa (HepG2)
alpha-Galactosidase A/GLA Primary R&D Systems
AF6146
alpha-Galactosidase A/GLA Antibody [Unconjugated] Sheep Polyclonal MCF-7 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line 0.2 mg/mL Size 50 µg/mL 55 kDa
alpha-Internexin Primary Novus Biologicals
NB300-140
alpha-Internexin Mouse Monoclonal SH-SY5Y lysate Size 1:200 140
alpha-Smooth Muscle Actin Primary Novus Biologicals
NBP2-33006
alpha-Smooth Muscle Actin Datasheet Mouse Monoclonal h. Aorta lysate Size 10 µg/mL 48-50

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