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Simple Western Antibody Database

Simple Western Certified Antibodies from R&D Systems, Novus Biologicals and More!

The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. The performance of any listed antibody will need to be optimized for the biological system being examined.

You can sort by column headings or just type into the Search box.

If you don't see your antibody listed here, shoot us an email at support@proteinsimple.com.

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Simple Western Validated Antibodies


Filter Antibodies: (3864)
Protein Target Antibody Type Vendor / Cat # Protein Isoform▲ Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
ADAMTS13 Primary R&D Systems
AF4245
Datasheet Sheep Polyclonal Size 1:50-1:300 129, 217
Anti Sheep-HRP Secondary R&D Systems
HAF016
Datasheet Donkey Polyclonal Size 1:50
Anti-Mouse-IgG-HRP Secondary R&D Systems
HAF018
Datasheet Donkey Polyclonal Size 1:100
MAP3K12 Primary Novus Biologicals
NBP2-17218
Datasheet Rabbit Polyclonal Mouse Retinal Ganglion Cells Size
β-catenin Primary Cell Signaling Technology
8480
Datasheet Rabbit Monoclonal HL60 Cell Lysates Size 1:25, 1:100, 1:400 85
β-catenin Primary Cell Signaling Technology
8480
Datasheet Rabbit Monoclonal HL60 Cell Lysates Size 1:25, 1:100, 1:400 85
AHCY Primary ABclonal
A5300
Citations (1)
Datasheet
Rabbit Polyclonal Size 1:130 Not_Stated
AMP-activated protein kinase (AMPK) Primary MilliporeSigma
07-350
Datasheet Rabbit Polyclonal L6 cells Size 1:10, 1:50, 1:250 64
AMP-activated protein kinase (AMPK) Primary MilliporeSigma
07-350
Datasheet Rabbit Polyclonal L6 cells Size 1:10, 1:50, 1:250 64
AMP-activated protein kinase (AMPK) Primary Cell Signaling Technology
2603S
Datasheet Mouse Monoclonal mouse brain tissue Size 1:50
Anti-goat-IgG-HRP Secondary Invitrogen
87-9363
Size 1:100-1:300
Anti-goat-IgG-HRP Secondary Thermo Fisher Scientific
31400
Charge 1:100
Anti-goat-IgG-HRP Secondary Zymax
81-1620
Charge 1:100-1:300
ATF-3 (D2Y5W) Primary Cell Signaling Technology
33593
Citations (1)
Datasheet
Rabbit Monoclonal Size
BCRP/ABCG2 Primary Abcam
ab207732
Rabbit Monoclonal Human K-562,K562/A02 Size Not_Stated 72kDa
BDNF Primary Abcam
ab108319
Rabbit Monoclonal Rat Retina Size Not_Stated 15kDa
beta Catenin Primary Abcam
ab16051
Rabbit Polyclonal Mouse HEI-OC1 Size 1:4000 92kDa
Beta-Actin Primary Li-Cor Biosciences
926-42210
Rabbit monoclonal Human neuroblastoma cells Size 1:200
Beta3-tubulin Primary Santa Cruz Biotechnology
sc-80005
Datasheet Mouse Monoclonal Neuron (RA induced in vitro neural differentiation of NE-4C) Size 1:50
Bim (C34C5) Primary Cell Signaling Technology
2933
Citations (1)
Datasheet
Mouse Monoclonal Size
Calponin 1 Primary Proteintech
13938-1-AP
Rabbit polyclonal human menstrual blood collection Size 1:50
Catenin-β (Y654), phospho Primary St John's Laboratory
STJ90209
Rabbit monoclonal Size 1:10 79
CD31 Primary Abcam
ab119339
Mouse Monoclonal Human Brain Size Not_Stated 231kDa
CDKN2A/p16INK4a Primary Abcam
ab108349
Rabbit Monoclonal Mouse HEI-OC1 Size 1:1000 16kDa
Choline acetyltransferase (ChAT) Primary Abcam
ab50412
Datasheet Goat Monoclonal Mouse brain tissue Size 1:10, 1:50, 1:250 70
Choline acetyltransferase (ChAT) Primary Abcam
ab50412
Datasheet Goat Monoclonal Mouse brain tissue Size 1:10, 1:50, 1:250 70
CHOP (L63F7) Primary Cell Signaling Technology
2895
Citations (1)
Datasheet
Mouse Monoclonal AsPC-1,PK-1 Size Not_Stated
Claudin 5 Primary Abcam
ab131259
Rabbit Monoclonal Human Brain Size Not_Stated 28kDa
Cyclin D Primary Cell Signaling Technology
2936
Datasheet Mouse Monoclonal HL60 Cell Lysates Size 1:25, 1:100, 1:400 38
Cyclin D Primary Cell Signaling Technology
2936
Datasheet Mouse Monoclonal HL60 Cell Lysates Size 1:25, 1:100, 1:400 38
EGFR (non-phospho-Tyr1173) Primary MilliporeSigma
05-484
Citations (1)
Datasheet
Mouse Monoclonal Null Charge 0.076388888888888895 Not_Stated
EGFR (non-phospho-Tyr1173) Primary MilliporeSigma
05-484
Citations (1)
Datasheet
Mouse Monoclonal Null Charge 0.076388888888888895 Not_Stated
Fatty Acid Binding Protein 5 Primary Abcam
ab128650
Rabbit Polyclonal Mouse Liver Charge Not_Stated 5.5, 5.6
FGFR3 Cell Signaling Technology
4574
Rabbit Monoclonal Human Spinal Cord Size 1:50 151 kDa
Fibrinogen Primary Aglient (DAKO)
A0080
Rabbit Polyclonal Citrated mouse plasma stimulated with α-thrombin Size 350
gamma H2A.X (phospho S139) Primary Abcam
ab26350
Mouse Monoclonal Mouse HEI-OC1 Size 1:1000 15kDa
Gankyrin Primary Abcam
ab182576
Rabbit Monoclonal Human Bone marrow Size 1:50 29kDa
GATA2 Primary Abcam
ab109241
Rabbit monoclonal CD34+ cells from human bone marrow Size
GFP Primary Affinity Biologicals
T0005
Mouse Monoclonal Transfected HEK293T & HeLa Size 1:50 25
Glucose transporter 2 (GLUT2) Primary Abcam
ab192599
Datasheet Rabbit Monoclonal Caco-2 cell Size 1:10, 1:50, 1:250 57-60
Glucose transporter 2 (GLUT2) Primary Abcam
ab192599
Datasheet Rabbit Monoclonal Caco-2 cell Size 1:10, 1:50, 1:250 57-60
Glycine Receptor alpha 1 + alpha 2 Primary Abcam
ab23809
Rabbit Polyclonal Rat Retina Size 1:100 52kDa
GNMT Primary ABclonal
A6608
Citations (1)
Datasheet
Rabbit Polyclonal Liver Size 1:130 Not_Stated
Goat Anti-Rabbit HRP Secondary Molecular Probes
A27036
Goat oligoclonal human menstrual blood collection Size 1:50
GSK3 beta Primary Abcam
ab69739
Rabbit Polyclonal Mouse Liver Charge Not_Stated 6.2, 8.8, 8.9, 9.3
HIF1a Primary Abcam
ab179483
Rabbit Monoclonal Hepatocytes Size 1:50 110
HIF2a Primary Abcam
ab199
Rabbit Polyclonal Hepatocytes Size 1:50 110
HSP70 Primary Cell Signaling Technology
4872
Rabbit polyclonal 3T3-L1 Charge 1:100
human RPS19 Primary Bethyl Laboratories
A304-002A
Datasheet Rabbit Polyclonal Hudep-2 cell line Size 1:100 24 kDa
ICAM1 Primary Proteintech
10831-1-AP
Datasheet Rabbit Polyclonal cell Size 1:20, 1:100, 1:500 95 KDa

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