Human/Mouse 4EBP1 Antibody

Name: Human/Mouse 4EBP1 Antibody
Brand: rnd
SKU: AF3227

Catalog # AF3227

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Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	E. coli-derived recombinant human 4EBP1 Ser2-Ile118 Accession # Q13541
Applications:	Knockout Validated, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human and mouse 4EBP1.

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Applications

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below

Item	Value
Knockout Validated	4EBP1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in 4EBP1 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human/Mouse 4EBP1 Antibody Scientific Data Examples

	Western Blot Detection of Human/Mouse 4EBP1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse 4EBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3227) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for 4EBP1 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human 4EBP1 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for 4EBP1 at approximately 26 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse 4EBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3227) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Knockout Validated Western Blot Shows Human 4EBP1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse 4EBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3227) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. New adjunct appears with knockout cell line.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 4EBP1

The eukaryotic initiation factor 4E (eIF-4E) binding protein 1 (4EBP1) binds to eIF-4E to prevent translation initiation. Upon phosphorylation by the target of rapamycin (TOR) kinase, 4EBP1 releases eIF-4E and translation initiation ensues. 4EBP1 is also known as PHAS-1 (phosphorylated heat and acid stable protein regulated by insulin 1).

Item	Value
Long Name	Eukaryotic Translation Initiation Factor 4E Binding Protein 1
Entrez Gene IDs	1978 (Human); 13685 (Mouse); 116636 (Rat)
Alternate Names	4E-BP1, 4EBP1, EIF4EBP1, PHAS-I, PHAS-IMGC4316, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1, eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1, eukaryotic translation initiation factor 4E-binding protein 1

Human/Mouse 4EBP1 Antibody

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Key Product Details

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Applications

Human/Mouse 4EBP1 Antibody Scientific Data Examples

Western Blot

Simple Western

Knockout Validated

Formulation, Preparation and Storage

Reconstitution Calculator

Background: 4EBP1

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COA

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