Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all 17 beta-HSD1/HSD17B1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human 17 beta-HSD1/HSD17B1
Ala2-Gln328
Accession # P14061

Specificity

Detects human 17 beta-HSD1/HSD17B1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human 17 beta-HSD1/HSD17B1 Antibody

Detection of Human 17 beta-HSD1/HSD17B1 antibody by Western Blot.

Detection of Human 17 beta-HSD1/HSD17B1 by Western Blot.

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human 17 beta-HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for 17 beta-HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
17 beta-HSD1/HSD17B1 antibody in Human Alzheimer's Brain by Immunohistochemistry (IHC-P).

17 beta‑HSD1/HSD17B1 in Human Alzheimer's Brain.

17 beta-HSD1/HSD17B1 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human 17 beta-HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human 17 beta-HSD1/HSD17B1 by Simple WesternTM.

Simple Western lane view shows lysates of human placenta, loaded at 0.2 mg/mL. A specific band was detected for 17 beta-HSD1/HSD17B1 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human 17 beta-HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human 17 beta-HSD1/HSD17B1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain

Simple Western

2.5 µg/mL
Sample: Human placenta

Western Blot

0.2 µg/mL
Sample: Human placenta tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 17 beta-HSD1/HSD17B1

HSD17B1 (Hydroxysteroid 17-beta dehydrogenase type 1; also 17  beta‑HSD1 and E2DH) is a 33-34 kDa member of the short chain dehydrogenase/reductase (SDR) superfamily of molecules. It is a cytoplasmic breast, placental and gonadal enzyme that both converts DHEA to estrogen A-diol, and inactivates DHT through oxidation and reduction. Human HSD17B1 is 328 amino acids (aa) in length. It contains an NADP binding site (aa 10-38) and a steroid catalytic site (aa 210-221). HSD17B1 functions as a 68 kDa noncovalently-linked homodimer. Over aa 2-328, human HSD17B1 shares 65% aa identity with mouse HSD17B1.

Long Name

17 beta Hydroxysteroid Dehydrogenase 1

Alternate Names

17 betaHSD1, E2DH, EDH17B2, EDHB17, HSD17B1, SDR28C1

Entrez Gene IDs

3292 (Human); 15485 (Mouse); 25322 (Rat)

Gene Symbol

HSD17B1

UniProt

P14061 (Human)

Additional 17 beta-HSD1/HSD17B1 Products

Product Documents for Human 17 beta-HSD1/HSD17B1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 17 beta-HSD1/HSD17B1 Antibody

For research use only

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