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Simple Western Antibody Database

Simple Western Certified Antibodies from R&D Systems, Novus Biologicals and More!

The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. The performance of any listed antibody will need to be optimized for the biological system being examined.

You can sort by column headings or just type into the Search box.

If you don't see your antibody listed here, shoot us an email at support@proteinsimple.com.

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Simple Western Validated Antibodies


Filter Antibodies: (3016)
Protein Target▲ Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
α-Nucleocapsid Primary Novus Biologicals
NB100-56683
SARS Nucleocapsid Protein Datasheet Rabbit Polyclonal Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2) Size 25 mg/mL 60 kDa
α-Synuclein Primary Cell Signaling Technology
2647
(α) alpha-Synuclein (Syn204) Mouse Monoclonal Spinal cord 3 ug/ul Size 1:10 22 kDa
α-Synuclein Primary Cell Signaling Technology
2642
(α) alpha-Synuclein Rabbit Polyclonal Size 1:25 21 kDa
α-Synuclein Primary Cell Signaling Technology
2647
(α) alpha-Synuclein (Syn204) Mouse Monoclonal Spinal cord 3 ug/uL Size 1:10 22 kDa
α-Synuclein, Phospho Primary Cell Signaling Technology
23706
Phospho (α) alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Spinal cord 3 ug/ul Size 1:250 23 kDa
α-Synuclein, Phospho Primary Cell Signaling Technology
93706
Phospho (α) alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Spinal cord 3 ug/uL Size 1:250 23 kDa
α-Tubulin Primary Millipore Sigma
T8203
alpha Tubulin Citations (1)
Mouse Monoclonal Transfected HeLa, Human Primary Fibroblast Lysates Size 1:100
β-actin Primary Millipore Sigma
A5441
(β) beta-actin Citations (2)
Mouse Monoclonal Rat Brain Lysate (0.2 mg/mL) Size 1:400 48 kDa
β-Actin Primary Millipore Sigma
A5411
(β) beta-actin Mouse Monoclonal Rat Brain Lysate (0.2 mg/mL) Size 1:400 48 kDa
β-Actin Primary GeneTex
GTX101794
Actin Gamma 1 Rabbit Polyclonal Human Stomach Smooth Muscles Size 1:100 44 kDa
β-Actin Primary Abcam
ab8224
(β) beta-Actin Citations (1)
Mouse Monoclonal Primate Corpus Luteum Size 1:100 49 kDa
β-Catenin Primary Cell Signaling Technology
8480
(β) beta-Catenin Rabbit Monoclonal NIH-3T3, A172, U87MG, U251 Size 1:250 90 kDa
β-Catenin Primary Cell Signaling Technology
9562
(β) beta-Catenin Rabbit Polyclonal HeLa Size 1:50
β-Catenin, Phospho Primary Cell Signaling Technology
2009
(β) beta-Catenin, Phospho S33/37 Rabbit Polyclonal NIH-3T3, A172, U87MG, U251 Size 1:50 92 kDa
β-Catenin, Phospho Primary Cell Signaling Technology
9561
(β) beta-Catenin, S33/37/T41 Rabbit Polyclonal NIH-3T3, A172, U87MG, U251 Size 1:50 92 kDa
β-Tubulin Primary Abcam
ab15568
Human (β) beta Tubulin aa 400 to the C-terminus Rabbit Polyclonal Mouse embryonic fibroblasts Size 1:50
β-Tubulin Primary Millipore Sigma
T4026
(β) beta-Tubulin Mouse Monoclonal Huh7.5.1 Size
14-3-3 γ Primary Novus Biologicals
NBP2-27202
14-3-3 gamma (γ) Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:50 33 kDa
14-3-3 γ Primary Novus Biologicals
NBP2-27370
14-3-3 gamma (γ) Datasheet Rabbit Polyclonal Human Brain lysate 0.5 mg/ml Size 1:10 34 kDa
14-3-3 γ Primary Novus Biologicals
NB100-406
14-3-3 gamma (γ) Datasheet Mouse Monoclonal HeLa lysate 1.0 mg/ml Size 1:12.5 36 kDa
14-3-3 ζ Primary R&D Systems
MAB2669
14-3-3 zeta (ζ) Datasheet Mouse Monoclonal SH-SY5Y human neuroblastoma cell line Size 2 µg/mL 34 kDa
15-PGDH/HPGD Primary Novus Biologicals
NB200-179
15-PGDH/HPGD Datasheet Rabbit polyclonal Lovo (left) and HeLa (right) lysate 0.5 mg/ml Size 1:500
15-PGDH/HPGD Primary R&D Systems
AF5660
15-PGDH/HPGD Datasheet Goat Polyclonal Human liver tissue Size 10 µg/mL 34 kDa
17 beta-HSD1/HSD17B1 Primary R&D Systems
MAB7178
17 beta-HSD1/HSD17B1 Datasheet Mouse Monoclonal Human placenta tissue Size 2.5 µg/mL 40 kDa
4-Hydroxynonenal Primary R&D Systems
MAB3249
4-Hydroxynonenal Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) Size 5 µg/mL
4E-BP1, phospho Primary Cell Signaling Technology
9455
Phospho 4E-BP1 (T70) Rabbit polyclonal Human Liver Tissue Charge
4EBP1 Primary R&D Systems
AF3227
4EBP1 Datasheet Goat Polyclonal HeLa human cervical epithelial carcinoma cell line Size 5 µg/mL 26 kDa
4EBP1 Primary Cell Signaling Technology
9644
4EBP1 Rabbit monoclonal MCF7 lysed in Bicine/CHAPS Size 1:50-1:100 28-32
4EBP1 Primary Cell Signaling Technology
4923
Non-p4EBP1 (Thr46) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Cell Signaling Technology
9644
4EBP1 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:200-1:1000
4EBP1 Primary Epitomics
1557-1
4EBP1 Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Millipore Sigma
07-1416
4EBP1 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Cell Signaling Technology
9452
4EBP1 Citations (1)
Rabbit polyclonal Caco2 cell lysates Size 1:50
4EBP1, phospho Primary Cell Signaling Technology
9451
p4EBP1 (Ser65) Citations (1)
Rabbit polyclonal K562 -/+ Gleevec lysed in Bicine CHAPS Size 1:50 27-31
4EBP1, phospho Primary Cell Signaling Technology
9459
p4EBP1(Thr37/46) Rabbit polyclonal K562 -/+ Gleevec lysed in Bicine CHAPS Size 1:50 27-31
4EBP1, phospho Primary Cell Signaling Technology
9459
p4EBP1 (Thr37/46) Rabbit polyclonal MCF7 lysed in Bicine/CHAPS Charge 1:50-1:100
4EBP1, phospho Primary Cell Signaling Technology
2855
p4EBP1 (Thr37/46) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9451
p4EBP1 (Ser65) Citations (1)
Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9456
p4EBP1 (Ser65) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9459
p4EBP1 (Thr37/46) Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Epitomics
2334-1
p4EBP1 (Thr45) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP2 Primary Cell Signaling Technology
2845
4EBP2 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:100
4HPPD Primary Proteintech
17004-1-AP
4-HPPD Rabbit polyclonal recombinant protein expression in E. coli Size 1:50 52
5'-Nucleotidase/CD73 Primary R&D Systems
AF5795
5'-Nucleotidase/CD73 Datasheet Sheep Polyclonal U266 human myeloma cell line Size 10 µg/mL 86
5-HT2AR Primary Lifespan Biosciences
LS-C172270
5-HT2AR Citations (1)
Rabbit polyclonal Rat cortex Size 1:500
5-HT7 Primary Novus Biologicals
NB100-56352
5-Hydroxytryptamine receptor 7 Rabbit Polyclonal Rat Brain Lysate Size 20 µg/mL 59 kDa
5-Lipoxygenase Primary Novus Biologicals
NB110-58748
5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 50 µg/mL
5-Lipoxygenase Primary Novus Biologicals
NB110-58749
5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 10 µg/mL
5HT1D Primary Novus Biologicals
NB100-56349
5HT1D Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:10
5HT2C (5-hydroxytryptamine Receptor 2C)/Serotonin Receptor 2C Primary Santa Cruz Biotechnology
sc-17797
5-HT2CR Mouse Rat brain fractions Size 1:50 55

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