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Simple Western Antibody Database

Simple Western Certified Antibodies from R&D Systems, Novus Biologicals and More!

The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. The performance of any listed antibody will need to be optimized for the biological system being examined.

You can sort by column headings or just type into the Search box.

If you don't see your antibody listed here, shoot us an email at support@proteinsimple.com.

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Simple Western Validated Antibodies


Filter Antibodies: (3016)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation▲ Dilution kDa on Simple Western
COX4 Primary Novus Biologicals
NBP2-43540
COX4 Mouse monoclonal Titrated to saturation using various models - 6 µg/mL
FCRN primary Novus Biologicals
NBP1-89127
FCRN Rabbit Polyclonal hepatocyte - 1:50 64
BChE Primary Santa Cruz Biotechnology
sc-377403
Specific for aa 85-119 near the N-terminus of BChE Mouse Monoclonal Yeast - 1:20 106 kDa
CSF1R Primary Cell Signaling Technology
3152
M-CSF Receptor Rabbit Polyclonal Mouse Macrophages, Mouse Myeloid Leukemia Cell Line (RIPA lysis buffer: 214, 165, 59 kDa; active motif nuclear extact kit whole-cell lysis buffer: 189, 59 kDa) - 1:25 59, 165, 189, 214
Donkey Anti-Mouse AlexFluor® 647 Secondary Thermo Fisher Scientific
A-31571
Anti-Mouse IgG (H+L); Jess Only Donkey Polyclonal - 1:100
GAPDH Primary Abcam
ab8245
GAPDH Mouse monoclonal - 1:50 40
GluR1 Primary Millipore
AB1504
Glutamate receptor 1 Rabbit Polyclonal Mouse brain lysate - 1:17 127
Hemagglutinin 2 Primary Pharma Foods International
HA2#16
HA2 Chicken monoclonal Viral particles in chicken eggs - 1.8 µg/mL 103 kDa
I kappa B alpha (IKBA) Primary Cell Signaling Technology
9242
IκBα (IKBA) Rabbit Polyclonal Brain homogenate - 1:50
ABCA1 Primary Novus Biologicals
NB400-105
ABCA1 Rabbit polyclonal Charge 1:50
c-Rel Primary Novus Biologicals
NB600-474
c-Rel Antibody Rabbit Polyclonal HEK-/+UV, whole cell lysate Charge 1:50
Caspase-3 Primary Novus Biologicals
NB100-1664
Caspase-3 Antibody Rabbit Polyclonal K562 cells -/+ Imatinib Charge 1:100
cIAP Primary R&D Systems
AF8181
cIAP Citations (1)
Datasheet
Goat polyclonal SKOV3, PMBC Charge 5 µg/mL
GRASP55 Primary Novus Biologicals
NB100-74431
GRASP55 Antibody Rabbit Polyclonal HeLa lysate Charge 1:50
HGF Primary R&D Systems
AF2207
HGF Goat polyclonal AML cells Charge 1:100
HSPA8/HSC71/Hsc70 Primary Novus Biologicals
NBP2-12880
HSPA8/HSC71/Hsc70 Antibody (1F2-H5) Mouse Monoclonal Charge 1:200
IKK beta Primary Novus Biologicals
NB600-477
IKK beta Antibody Rabbit Polyclonal HeLa-/+EGF, Jurkat/TNF Charge 1:50
IKK epsilon/IKBKE Primary Novus Biologicals
NBP1-77829
IKK epsilon/IKBKE [p Thr501] Antibody Rabbit Polyclonal HeLa-/+EGF, Jurkat/TNF Charge 1:50
MK2 Primary Novus Biologicals
H00009261-M01
MK2 Mouse monoclonal Charge 1:50
MK3 Primary Novus Biologicals
H00007867-M01
MK3 Mouse monoclonal Charge 1:50
p70 S6 Kinase/S6K Primary Novus Biologicals
NB100-293
p70 S6 Kinase/S6K Antibody Rabbit Polyclonal treated and untreated MCF7 Charge 1:50
Phospho Her3/ErbB3 Primary R&D Systems
AF5817
Phospho Her3/ErbB3 Rabbit MCF7 lysates Charge
PSA Primary R&D Systems
AF1344
Human Kallikrein3/PSA Goat polyclonal Purified Native PSA from human seminal fluid (Calbiochem Cat#539832) Charge 1:50
RelA/NFkB p65 Primary Novus Biologicals
NBP1-77808
RelA/NFkB p65 [p Ser529] Antibody Rabbit Polyclonal HeLa-/+TNF, HeLa-/+TPA, nuclear extracts (Active Motif) Charge 1:50
RelA/NFkB p65 Primary Novus Biologicals
NBP1-77807
RelA/NFkB p65 [p Ser276] Antibody Rabbit Polyclonal HeLa-/+TNF, HeLa-/+TPA, nuclear extracts (Active Motif) Charge 1:50
RPA2 Primary Novus Biologicals
NBP1-23017
RPA2 [p Ser4, p Ser8] Antibody Rabbit Polyclonal Human squamous carcinoma cells Charge 1:50
RPA2 Primary Novus Biologicals
NB100-332
RPA2 Antibody Rabbit Polyclonal Human squamous carcinoma cells Charge 1:500
RPA2 Primary Novus Biologicals
NB100-544
RPA2 [p Ser33] Antibody Rabbit Polyclonal Human squamous carcinoma cells Charge 1:25
S6 Ribosomal Protein Primary R&D Systems
AF3918
pS6 Ribosomal Protein (Ser235/236) Datasheet Rabbit polyclonal MCF-7 Charge 1:50
Src Primary Novus Biologicals
NBP1-19188
Src Antibody (5A18) Mouse Monoclonal A431 -/+ EGF Charge 1:50
Transferrin Primary R&D Systems
MAB5746
Transferrin Mouse Monoclonal Human Holo-Transferrin Protein CF (R&D Systems PN 2914-HT), Normal CDT Control (Sebia PN 4795) 1/200, High CDT Control (Sebia PN 4772) 1/200 Charge 1:200
Transferrin Primary R&D Systems
MAB5746
Transferrin Mouse Monoclonal Human Holo-Transferrin Protein CF (R&D Systems PN 2914-HT), Normal CDT Control (Sebia PN 4795) 1/200, High CDT Control (Sebia PN 4772) 1/200 Charge 1:200
4E-BP1, phospho Primary Cell Signaling Technology
9455
Phospho 4E-BP1 (T70) Rabbit polyclonal Human Liver Tissue Charge
4EBP1 Primary Cell Signaling Technology
4923
Non-p4EBP1 (Thr46) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Cell Signaling Technology
9644
4EBP1 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:200-1:1000
4EBP1 Primary Epitomics
1557-1
4EBP1 Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Millipore Sigma
07-1416
4EBP1 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9459
p4EBP1 (Thr37/46) Rabbit polyclonal MCF7 lysed in Bicine/CHAPS Charge 1:50-1:100
4EBP1, phospho Primary Cell Signaling Technology
2855
p4EBP1 (Thr37/46) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9451
p4EBP1 (Ser65) Citations (1)
Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9456
p4EBP1 (Ser65) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9459
p4EBP1 (Thr37/46) Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Epitomics
2334-1
p4EBP1 (Thr45) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP2 Primary Cell Signaling Technology
2845
4EBP2 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:100
Acetylated Lysine Primary Cell Signaling Technology
9441
Acetylated-Lysine Rabbit Polyclonal Huh7.5 Charge 1:50
AKT Primary Cell Signaling Technology
2920
AKT1/2/3 Citations (1)
Mouse monoclonal MEF and NHLF Charge 1:100
AKT Primary Cell Signaling Technology
4691
AKT Citations (5)
Datasheet
Rabbit monoclonal MCF7 lysed in Bicine/CHAPS Charge 1:50-1:100
AKT Primary Cell Signaling Technology
9272
AKT1/2/3 Citations (3)
Rabbit polyclonal EGF stimulated MCF-7 Charge 1:50
AKT, phospho Primary Cell Signaling Technology
9271
pAKT (Ser473) Citations (3)
Rabbit polyclonal EGF stimulated MCF-7 Charge 1:100
AKT, phospho Primary Cell Signaling Technology
4060
pAKT (Ser473) Citations (4)
Rabbit monoclonal A549 Charge 1:50

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