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Human/Mouse/Rat 11 beta-HSD1 Antibody

Bio-Techne includes R&D Systems | Catalog # AF3397

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AF3397
AF3397-SP

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rabbit

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human/Mouse/Rat 11 beta-HSD1 Antibody

Immunogen

E. coli-derived recombinant human 11 beta-HSD1
Asn24-Lys292
Accession # P28845

Specificity

Detects endogenous human, mouse and rat 11 beta-HSD1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat 11 beta-HSD1 Antibody

Detection of Human/Mouse/Rat 11 beta-HSD1 antibody by Western Blot.

Detection of Human/Mouse/Rat 11 beta-HSD1 by Western Blot.

Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat 11 beta-HSD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3397) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for 11 beta-HSD1 at approximately 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat 11 beta-HSD1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human, mouse and rat liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 6 publications using AF3397 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 11 beta-HSD1

Hydoxysteroid (11-beta) Dehydrogenase, type I 11-beta-hydroxysteroid dehydrogenase 1(11 beta-HSD1, HSD11B1 or HSD1) is a microsomal glycoprotein enzyme, with an apparent mass of 36 kDa, that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. 11 beta-HSD1 can also catalyze the reverse reaction, the conversion of cortisone to cortisol. 11 beta-HSD1 has been detected in a wide range of rat and human tissues, including lung and testis, but highest amounts are expressed in the liver. Human 11 beta-HSD1 shares 79 and 77% sequence identity with mouse and rat 11 beta-HSD1, respectively. There are at least 2 isoforms of 11 beta-HSD. The other isoform, type II, is expressed predominantly in the kidney and placenta and catalyzes only the 11-beta-dehydrogenation reaction. High levels of cortisol can lead to visceral obesity and particular defects in the expression or function of 11 beta-HSD1 have also been associated with insulin resistance and cognitive function.

Long Name

11 beta Hydroxysteroid Dehydrogenase 1

Alternate Names

11 betaHSD1, HSD11B1

Entrez Gene IDs

3290 (Human); 628438 (Mouse); 24470 (Rat)

Gene Symbol

HSD11B1

UniProt

Additional 11 beta-HSD1 Products

Product Documents for Human/Mouse/Rat 11 beta-HSD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat 11 beta-HSD1 Antibody

For research use only

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