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4-Hydroxynonenal Antibody
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Bio-Techne Corporation
tel: (612) 379-2956
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$35 to United States
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Key Product Details
| Immunogen: | KLH-coupled 4-Hydroxynonenal |
| Applications: | Immunohistochemistry, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG2B Clone # 198960 |
| Specificity: |
Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed. |
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Western BlotDetection of Human 4‑Hydroxynonenal by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for 4-Hydroxynonenal at adducts of histidine residues (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Simple WesternDetection of Human 4‑Hydroxynonenal by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
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Immunohistochemistry4‑Hydroxynonenal in Human Prostate. 4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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Immunohistochemistry4‑Hydroxynonenal in Human Prostate Cancer Tissue. 4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
Citations for 4-Hydroxynonenal Antibody (28)
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Citations are publications that use Bio-Techne products. Selected citations for 4-Hydroxynonenal Antibody include:
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Species: Rat
Sample Types: Whole Tissue
Applications: IHCM Baškovi? et al. (2022), Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury-Immunohistochemical and Biochemical Analyses Journal of Clinical Medicine, 11(5).
PMID: 35268375 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCHH Dieguez et al. (2021), Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration Cell Death Disease, 2021;12(12):1128.
PMID: 34864827 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCJ Misra et al. (2021), Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress Nucleic Acids Research, 49(10):5726-5742.
PMID: 34023907 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCD Das et al. (2021), Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPAR&gamma binodal complementary modules Redox Biology, 45(0):102043.
PMID: 34144391 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotCS Bruells et al. (2021), Acute liver injury following acetaminophen administration does not activate atrophic pathways in the mouse diaphragm Scientific Reports, 11(1):6302.
PMID: 33737702 -
Species: Mouse
Sample Types: Tissue
Applications: IHC-PM Li et al. (2020), Sirt3 modulates fatty acid oxidation and attenuates cisplatin-induced AKI in mice J. Cell. Mol. Med., 0(0).
PMID: 32281286 -
Species: Mouse
Sample Types: Cell Lystates
Applications: Western BlotH Tendilla-B et al. (2019), Risperidone ameliorates prefrontal cortex neural atrophy and oxidative/nitrosative stress in brain and peripheral blood of rats with neonatal ventral hippocampus lesion J. Neurosci., 0(0).
PMID: 31519825 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotP Siriviriya et al. (2019), Effects of curcumin on oxidative stress, inflammation and apoptosis in L-arginine induced acute pancreatitis in mice Heliyon, 5(8):e02222.
PMID: 31485503 -
Species: Ovine
Sample Types: Tissue Homogenates
Applications: Western BlotF Liang et al. (2019), Mechanical ventilation causes diaphragm dysfunction in newborn lambs Crit Care, 23(1):123.
PMID: 30992039 -
Species: Rat
Sample Types: Whole Tissue
Applications: Western BlotJM Gemechu et al. (2018), Characterization of Dopaminergic System in the Striatum of Young Adult Park2-/- Knockout Rats Sci Rep, 8(1):1517.
PMID: 29367643 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotH Lee et al. (2018), Tocotrienol-rich fraction supplementation reduces hyperglycemia-induced skeletal muscle damage through regulation of insulin signaling and oxidative stress in type 2 diabetic mice J. Nutr. Biochem., 57(0):77-85.
PMID: 29679925 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotNG Barra et al. (2018), Maternal Nicotine Exposure (MNE) leads to augmented expression of the anti-oxidant adipose tissue triglyceride lipase (ATGL) long-term in the white adipose of female rat offspring Toxicol. Sci., 0(0).
PMID: 29617909 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrA Chatterjee et al. (2018), The Addition of Manganese Porphyrins during Radiation Inhibits Prostate Cancer Growth and Simultaneously Protects Normal Prostate Tissue from Radiation Damage Antioxidants (Basel), 7(1).
PMID: 29370088 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotWS Hambright et al. (2017), Ablation of ferroptosis regulator glutathione peroxidase 4 in forebrain neurons promotes cognitive impairment and neurodegeneration Redox Biol, 12(0):8-17.
PMID: 28212525 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotJ Zhao et al. (2017), Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice J. Biol. Chem., 0(0).
PMID: 29191837 -
Species: Mouse
Sample Types: Plasma
Applications: Western BlotJ Shin et al. (2017), Gamma-tocopherol supplementation ameliorated hyper-inflammatory response during the early cutaneous wound healing in alloxan-induced diabetic mice Exp. Biol. Med. (Maywood), 242(5):505-515.
PMID: 28211759 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotJ Seo et al. (2016), Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene PLoS ONE, 11(4):e0154390.
PMID: 27111068 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotGarate I et al. (2014), Toll-like 4 receptor inhibitor TAK-242 decreases neuroinflammation in rat brain frontal cortex after stress. J Neuroinflammation, 11(0):8.
PMID: 24410883 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotHolloway et al. (2014), Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies. Am J Physiol Endocrinol Metab, 306(4):E443-56.
PMID: 24368670 -
Species: Drosophila
Sample Types: Whole Tissue
Applications: IHCBufalino et al. (2013), The asymmetric segregation of damaged proteins is stem cell-type dependent. J Cell Biol, 201(4):523-30.
PMID: 23649805 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotMargaritis M et al. (2013), Interactions between vascular wall and perivascular adipose tissue reveal novel roles for adiponectin in the regulation of endothelial nitric oxide synthase function in human vessels. Circulation, 127(22):2209-21.
PMID: 23625959 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot(2012), Transgenic control of mitochondrial fission induces mitochondrial uncoupling and relieves diabetic oxidative stress. Diabetes, 61(8):2093-104.
PMID: 22698920 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot(2012), Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in pre-clinical models of Parkinson's disease. J Neuroinflammation, 9(0):241.
PMID: 23092448 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotWang Z et al. (2011), 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARgamma and accelerating ubiquitin-proteasome degradation. Mol. Cell. Endocrinol., 349(2):222-31.
PMID: 22085560 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotYano M et al. (2011), Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice. J. Biol. Chem., 286(5):3992-4002.
PMID: 21115496 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotWalson KH et al. (2011), Normoxic versus hyperoxic resuscitation in pediatric asphyxial cardiac arrest: effects on oxidative stress. Crit. Care Med., 39(2):335-43.
PMID: 21057313 -
Species: Rat
Sample Types: Whole Cells
Applications: ICCStraface E et al. (2009), Cell sex determines anoikis resistance in vascular smooth muscle cells. FEBS Lett., 583(21):3448-54.
PMID: 19804776 -
Species: Human
Sample Types: Whole Cells
Applications: ICCMargutti P et al. (2007), Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis. Blood, 111(9):4559-70.
PMID: 17993611
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Application: IHCSample Tested: Liver tissueSpecies: MouseVerified Customer | Posted 08/11/2021
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Application: ICC/IFSample Tested: Aortic valvesSpecies: MouseVerified Customer | Posted 03/29/2021-Fixed in 10% neutral buffered formalin followed by dehydration process-10 um thick cryosection- Blocking: Normal Goat Serum (NGS)- Primary Ab (4-HNE): diluted in NGS at 20 ug/mL; 4C overnight- Secondary (AF488): 1:100 dilution in NGS; room temp 1hr- Vector anti-fade mounting media with DAPI
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