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Human 14‑3‑3 zeta Antibody

Bio-Techne includes R&D Systems | Catalog # MAB2669

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MAB2669
MAB2669-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 818515

Product Summary for Human 14‑3‑3 zeta Antibody

Immunogen

E. coli-derived recombinant human 14-3-3 zeta
Asp2-Asn245
Accession # P63104

Specificity

Detects human 14-3-3 zeta in direct ELISAs and Western blots. Detects Mouse and Rat 14-3-3 zeta in Western Blots. In direct ELISAs, nocross-reactivity with recombinant human 14-3-3 beta, theta, eta, gamma, sigma, or epsilon is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human 14‑3‑3 zeta Antibody

Detection of Human, Mouse, and Rat 14-3-3? antibody by Western Blot.

Detection of Human, Mouse, and Rat 14‑3‑3 zeta by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, SK-Mel-28 human malignant melanoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human 14-3-3 zeta Monoclonal Antibody (Catalog # MAB2669) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 14-3-3 zeta at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
14-3-3? antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P).

14‑3‑3 zeta in Human Squamous Cell Carcinoma.

14-3-3 zeta was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human 14-3-3 zeta Monoclonal Antibody (Catalog # MAB2669) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei and plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human 14-3-3? antibody by Simple WesternTM.

Detection of Human 14‑3‑3 zeta by Simple WesternTM.

Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line, loaded at 0.5 mg/mL. A specific band was detected for 14‑3‑3 zeta at approximately 34 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human 14‑3‑3 zeta Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human squamous cell carcinoma

Simple Western

2 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line

Western Blot

0.2 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, SK‑Mel‑28 human malignant melanoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB2669 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 zeta

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins were the first polypeptides shown to have pSer/Thr binding properties, generally recognizing the consensus sequences RSXpSXP and RXY/FXpSXP (where X is any amino acid). 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. For example, the binding of 14-3-3 to phosphorylated BAD blocks its proapoptotic association with Bcl-XL. There are at least seven distinct 14-3-3 genes in vertebrates, alpha/beta, epsilon, eta, gamma, theta, sigma and zeta ( alpha/ beta, epsilon, eta, gamma, tau/ theta, sigma, and zeta/ delta). 14-3-3 zeta, also known as Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, zeta isoform (gene name YWHAZ) is a 245 amino acid, 27 kDa protein.

Alternate Names

1433 zeta, KCIP-1, YWHAZ

Entrez Gene IDs

7534 (Human)

Gene Symbol

YWHAZ

UniProt

Additional 14-3-3 zeta Products

Product Documents for Human 14‑3‑3 zeta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human 14‑3‑3 zeta Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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