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Simple Western Antibody Database

Simple Western Certified Antibodies from R&D Systems, Novus Biologicals and More!

The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. The performance of any listed antibody will need to be optimized for the biological system being examined.

You can sort by column headings or just type into the Search box.

If you don't see your antibody listed here, shoot us an email at support@proteinsimple.com.

Help scientists and fund you research. Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about this offer at Simple Western Antibody Submission Promotion.

Simple Western Validated Antibodies


Filter Antibodies: (3016)
Protein Target Antibody Type Vendor / Cat #▲ Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
β-Actin Primary Abcam
ab8224
(β) beta-Actin Citations (1)
Mouse Monoclonal Primate Corpus Luteum Size 1:100 49 kDa
β-Tubulin Primary Abcam
ab15568
Human (β) beta Tubulin aa 400 to the C-terminus Rabbit Polyclonal Mouse embryonic fibroblasts Size 1:50
A RAF Primary Abcam
ab200653
A RAF Rabbit Monoclonal A375 cell line Size 1:50
ABCA1 Primary Abcam
ab18180
ABCA1 Mouse Monoclonal Mouse liver lysate Size 1:50 270 kDa
ABCG1 Primary Abcam
ab52617
ABCG1 Rabbit monoclonal Mouse liver lysate Size 1:50 110 (mouse) -116kDa (human)
ABCG2 Primary Abcam
ab24115
ABCG2 Rat monoclonal 3T3, MCF7, H460 Size 1:20 100
Actin β Primary Abcam
ab8226
Cytoskeleton Mouse Monoclonal HeLa, Jurkat, HEK 293 Size 1:50 48
Adenosine Receptor A2a Primary Abcam
ab3461
Adenosine Receptor A2a Rabbit Polyclonal Size 1:50 39
ADH5 Primary Abcam
ab59134
ADH5 Goat Polyclonal Human uterine tissue Size 1:100
AKT1/2/3 Primary Abcam
ab32505
AKT1/2/3 Rabbit Monoclonal Mouse aortas and HUVEC lysate Size 1:100
Alkaline Phosphatase Primary Abcam
ab108337
Alkaline Phosphatase Rabbit Monoclonal ADSCs, FBs, BMSCs Size 1:50 57
Androgen Receptor Primary Abcam
ab52615
Androgen Receptor Rabbit Monoclonal Size 120 kDa
Androgen Receptor Primary Abcam
ab52615
Androgen Receptor Rabbit Monoclonal Size 120 kDa
Androgen Receptor Primary Abcam
ab198394
AR-V7 Rabbit monoclonal Human Plasma Size 1:50 97
Angiopoietin 2/ANG2 Primary Abcam
ab155106
Angiopoietin 2/ANG2 Rabbit Monoclonal Untreated A549 Lysate 0.2 mg/mL, A549 Lysate Treated with 1% O2 0.2 mg/mL, A549 Lysate Treated with CoCL2 (4h) 0.2 mg/mL Size 1:50 39/56/95/145
Angiopoietin 2/ANG2 Primary Abcam
ab155106
Angiopoietin 2/ANG2 Rabbit Monoclonal Untreated A549 Lysate 0.2 mg/mL, A549 Lysate Treated with 1% O2 0.2 mg/mL, A549 Lysate Treated with CoCL2 (4h) 0.2 mg/mL Size 1:50 39/56/95/145
APC Primary Abcam
ab154906
APC Rabbit polyclonal Hela,K562 Size 1:50
APP Primary Abcam
ab32136
Amyloid Precursor Protein Rabbit Monoclonal Size 1:200 129, 170
ATM, Phospho Primary Abcam
ab81292
ATM, Ser1981 Citations (1)
Rabbit Monoclonal Transfected HeLa, Human Primary Fibroblast Lysates Size 1:100
ATP Synthase Primary Abcam
ab96655
ATP Synthase Rabbit Polyclonal Human bladder tumor cells (T24 cells) and human skin fibroblasts (CAFs) Size
ATPB Primary Abcam
ab14730
ATPB Mouse monoclonal Mouse brain endothelioma cell line (bEnd5) Size 1:500
Aurora B Primary Abcam
ab45145
Aurora B Rabbit Monoclonal TK6 cells Size 1:50
B Raf Primary Abcam
ab33899
B Raf Rabbit Monoclonal A375 cell line Size 1:50
BCKDHA Primary Abcam
ab138460
BCKDA Rabbit polyclonal Bone Tissue Extracts Size 1:50 50
Bcl-XL Primary Abcam
ab32370
Bcl-XL Rabbit monoclonal Size 1:50 34 kDa
BDNF Primary Abcam
ab10505
BDNF Mouse monoclonal Human Saliva Charge 1:50
BDNF Primary Abcam
ab10505
BDNF Mouse monoclonal Human Plasma Size 1:50
beta Actin Primary Abcam
ab8227
beta Actin Citations (1)
Rabbit, polyclonal mouse oocytes Size 1:50 47
Beta actin Primary Abcam
Ab6276
Beta actin Citations (1)
Mouse monoclonal Size 1:25-1:100
Beta-2 Microglobulin Primary Abcam
ab75853
Beta-2 Microglobulin Rabbit monoclonal Many Charge 1:50
BLK, Phospho Primary Abcam
ab192670
Phospho Tyr501 Rabbit Polyclonal Mouse splenocytes +/- LCWE treatment Size
BRCA1, Phospho Primary Abcam
ab90528
BRCA1, Ser1423 Citations (1)
Rabbit Polyclonal Transfected HeLa, Human Primary Fibroblast Lysates Size 1:100
Cadherin Primary Abcam
Ab6528
Pan cadherin Mouse monoclonal Rat cortex Size 1:1000
Caspase 3 Primary Abcam
ab2302
Cleaved Caspase 3 Rabbit polyclonal K562 cells -/+ Imatinib Charge 1:25-1:100
Caspase 3 Primary Abcam
ab32042
Cleaved Caspase 3 Rabbit monoclonal K562 cells -/+ Imatinib Charge 1:50
CD4 Primary Abcam
ab133622
CD4 Rabbit Monoclonal Treg cell lysate 0.04 mg/mL, PBMC lysate 0.04 mg/mL Size 1:250 64
CD4 Primary Abcam
ab133622
CD4 Rabbit Monoclonal Treg cell lysate 0.04 mg/mL, PBMC lysate 0.04 mg/mL Size 1:250 64
CD63 Primary Abcam
ab68418
CD63 Rabbit Polyclonal Human A549 Cells (Novus Biologicals PN NBP2-49862) PNGase F Treated 0.2 mg/mL Size 1:10 28
CD83 Primary Abcam
ab170855
CD83 Rabbit Monoclonal Human monocyte-derived dentritic cells 0.5 mg/mL Size 1:10 44/52/56/77/137
CD83 Primary Abcam
ab170855
CD83 Rabbit Monoclonal Human monocyte-derived dentritic cells 0.5 mg/mL Size 1:10 44/52/56/77/137
CGRP Primary Abcam
ab189786
CGRP Rabbit polyclonal Human Saliva Charge 1:25
CGRP Primary Abcam
ab189786
CGRP Rabbit polyclonal Human Plasma Size 1:25
cMet Primary Abcam
ab51067
cMet Rabbit monoclonal Human brain microvascular endothelial cells Size
Collagen III Primary Abcam
ab7778
Collagen III Rabbit polyclonal Skin Size 1:50 139
Collagen Type I Primary Abcam
ab34710
Collagen I Rabbit polyclonal Cultured adult normal human dermal fibrblast cells (NHDF) Size 1:1000
Complex II (succinate dehydrogenase) Primary Abcam
ab14715
SDHA Mouse monoclonal Rat heart/testes lysates and mitochondrial fractions Size 1:50-1:250 65
Complex IV (Cytochrome C oxidase) Primary Abcam
ab14705
MTCO1 Mouse monoclonal Rat heart/testes lysates and mitochondrial fractions Size 1:50 45
CRKL, phospho Primary Abcam
ab52908
pCRKL (Tyr207) Citations (1)
Rabbit monoclonal K562 cells -/+ Imatinib Charge 1:50
CXCL16 Primary Abcam
ab101404
CXCL16 Rabbit polyclonal Macrophages Size
Cyclin D1 Primary Abcam
ab134175
Cyclin D1 Rabbit Monoclonal Human Multiple Myeloma Size 1:50 40

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