Skip to main content

Human 15‑PGDH/HPGD Antibody

Catalog # AF5660 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
AF5660
AF5660-SP

Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human 15‑PGDH/HPGD Antibody

Immunogen

E. coli-derived recombinant human 15-PGDH/HPGD
Met1-Gln266
Accession # P15428

Specificity

Detects human 15-PGDH/HPGD in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human 15‑PGDH/HPGD Antibody

Detection of Human 15-PGDH/HPGD antibody by Western Blot.

Detection of Human 15-PGDH/HPGD by Western Blot.

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human 15-PGDH/HPGD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for 15-PGDH/HPGD at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human 15-PGDH/HPGD antibody by Simple WesternTM.

Detection of Human 15‑PGDH/HPGD by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for 15-PGDH/HPGD at approximately 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Human 15-PGDH/HPGD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5660) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human 15‑PGDH/HPGD Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human liver tissue

Western Blot

1 µg/mL
Sample: Human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 15-PGDH/HPGD

HPGD, or 15-hydroxyprostaglandin dehydrogenase, is a NAD+-linked dehydrogenase that oxidizes the hydroxyl group at position 15 of prostaglandins to a ketone, resulting in a loss of biological activity (1). HPGD is a major enzyme for the catabolism of prostaglandins. The enzyme is a member of the short-chain alcohol dehydrogenase family of enzymes (2). HPGD is a cytosolic enzyme expressed in most tissues, with highest expression levels in placenta, lung, and kidney (3). It is inhibited by aspirin and nonsteroidal anti-inflammatory drugs (4). Defects in HPGD are a cause of hypertrophic osteoarthropathy (5).

References

  1. Anggard, E. and B. Samuelsson (1964) J. Biol. Chem. 239:4097.
  2. Krook, M. et al. (1990) Biochemistry 29:738.
  3. Tai, H.H. (1976) Biochemistry 15:4586.
  4. Mak, O.T. et al. (1982) Biosci. Rep. 2:503.
  5. Uppal, S. et al. (2008) Nat. Genet. 40:789.

Long Name

15-Hydroxyprostaglandin Dehydrogenase

Alternate Names

15PGDH, HPGD, PGDH1, SDR36C1

Entrez Gene IDs

3248 (Human); 15446 (Mouse); 79242 (Rat)

Gene Symbol

HPGD

UniProt

Product Documents for Human 15‑PGDH/HPGD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human 15‑PGDH/HPGD Antibody

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents