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Goat IgG Horseradish Peroxidase-conjugated Antibody

Catalog # HAF019 | R&D Systems, Inc. a Bio-Techne Brand
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HAF019

Key Product Details

Species Reactivity

Goat

Applications

Immunohistochemistry, Western Blot

Label

HRP

Antibody Source

Polyclonal Chicken IgY

Product Summary for Goat IgG Horseradish Peroxidase-conjugated Antibody

Immunogen

Goat IgG

Specificity

Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rabbit IgG and human IgG is observed.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Scientific Data Images for Goat IgG Horseradish Peroxidase-conjugated Antibody

Detection of Goat IgG Primary Antibody by Western Blot.

Detection of Goat IgG Primary Antibody by Western Blot.

Western blot shows detection of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Polyclonal Antibody (AF4920) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Vimentin in Human Kidney

Detection of Vimentin in human kidney using 5 ug/mL of Goat anti-human Vimentin primary antibody (AF2105) followed by 30 min incubation with Donkey Anti-Goat HRP conjugated secondary antibodies (Catalog# HAF019) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.

Applications for Goat IgG Horseradish Peroxidase-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Use at a 1:20 dilution 

Western Blot

Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using HAF019 in the following applications:

Published Applications

Read 21 publications using HAF019 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from egg yolks

Formulation

Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Product Documents for Goat IgG Horseradish Peroxidase-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Goat IgG Horseradish Peroxidase-conjugated Antibody

For research use only

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