Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

Name: Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody
Brand: R&D Systems
SKU: AF4920
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF4920

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Species Reactivity:	Human, Mouse, Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Carboxylesterase 1/CES1 His19-Glu563 Accession # NP_001020365
Applications:	Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Carboxylesterase 1/CES1 in direct ELISAs and Western blots. Detects mouse Carboxylesterase 1/CES1 and rat Carboxylesterase 1/CES1 in Western blots.

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Applications for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	2 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

	Western Blot Detection of Human, Mouse, and Rat Carboxylesterase 1/CES1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4920) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carboxylesterase 1/CES1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human and Mouse Carboxylesterase 1/CES1 by Simple Western^TM. Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Carboxylesterase 1/CES1 at approximately 62 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4920) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Carboxylesterase 1/CES1

Carboxylesterase 1 (CES1) is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). They have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They play a major role as determinants of pharmacokinetic behavior for most therapeutic agents containing an ester. By de-esterification, they can activate or inactivate the agents. They also participate in detoxification of drugs such as cocaine and heroin in serum and liver. The resulting de-esterified metabolites are secreted out in urine. They can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification, a reaction important for cholesterol homeostasis. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia. CES1 shares the serine hydrolase fold observed in other esterases (3). CES1 possesses an endoplasmic reticulum retention signal (HIEL) at its C-terminus.

Item	Value
References	Redinbo, M.R. and P.M. Potter (2005) Drug Discovery Today 10:313. Satoh, T. and M. Hosokawa (2006) Chem. Biol. Interactions 162:195. Fleming, C.D. et al. (2007) Biochemistry 46:5603.
Entrez Gene IDs	1066 (Human); 12623 (Mouse); 29225 (Rat)
Alternate Names	ACAT, Acyl-coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, CEH, CES1, CES1A2, CES2EC 3.1.1.1, Carboxylesterase 1, Ces-1, Cocaine carboxylesterase, EC 3.1.1, Egasyn, HMSE1, HMSECES1A1, MGC117365, Monocyte/macrophage serine esterase, PCE-1, REH, Retinyl ester hydrolase, SES1, SES1TGH, Serine esterase 1, Ses-1, TGH, Triacylglycerol hydrolase, acyl coenzyme A:cholesterol acyltransferase, carboxylesterase 1 (monocyte/macrophage serine esterase 1), carboxylesterase 2 (liver), cholesteryl ester hydrolase, human monocyte/macrophage serine esterase 1, liver carboxylesterase 1

Citations for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Murakami E et al. (2010), Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977. J. Biol. Chem., 285(45):34337-47.
PMID: 20801890

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Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

Name: Anonymous

Application: WB

Sample Tested: Caco-2 human colorectal adenocarcinoma cell line

Species: cell lysate

Verified Customer | Posted 02/17/2020

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Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

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Applications for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

Scientific Data Examples for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

Western Blot

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Carboxylesterase 1/CES1

Citations for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody (1)

Customer Reviews for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody (1)

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Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

Product Documents for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

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