GDP-Azido-Fucose
R&D Systems, part of Bio-Techne | Catalog # ES101
Activated Sugar
Key Product Details
Key Benefits
Learn more about Fluorescent Glycan Labeling and Detection
Species
Multi-Species
Conjugate
Azido
Product Summary for GDP-Azido-Fucose
|
Formula |
C16H24N8O15P2 |
|
Molecular Weight |
630.35 Da |
|
Formulation |
1 mM Provided in 20 mM Tris, 50 mM NaBorate, pH 9.0 |
Incorporate or detect fucose without expensive, specialized equipment!
Applications
- For in vitro enzymatic incorporation of azido-sugars into specific, targeted glycans.
- Detecting the presence or absence of fucosylated glycans.
- Tagging bio-molecules through fucosylation.
- Assessing the level of fucosylation on target molecules.
Key Features and Benefits
- Can assess for the presence of terminal fucosylation (e.g. core fucose) without expensive, specialized equipment.
- Can be introduced to proteins and lipids that have fucosylation sites via fucosyltransferases.
- Can be conjugated to desired reporter molecules via click chemistry.
- Can be detected via Western blot, ELISA, and flow cytometry, depending on the type of reporter molecule.
- Contains the smallest possible orthogonal functional group.
- Has minimal side effects on target molecules.
- User-friendly.
For Details:
Wu, ZL et al., (2015) Carbohydrate Res. 412:1-6.
Wu, ZL. et al., (2020) Glycobiology 30:970.
Related Reagents
Click Chemistry
Enzymes and Detection Reagents for GDP-Azido-Fucose, ES101
|
Fucosyltransferases |
Fucosylation |
Notes |
|---|---|---|
| Protein O-Fucosyltransferase 1/POFUT1 | Adds Fucose to Ser/Thr | Important for Notch signaling |
| Fucosyltransferase 2/FUT2 | Adds (terminal) Fucose to Galactose | Generates H antigen |
| Fucosyltransferase 3/FUT3 | Adds (terminal) Fucose to GlcNAc | Generates Lea, Leb antigens |
| Fucosyltransferase 5/FUT5 | Adds (terminal) Fucose to Galactose | Generates sLex antigen |
|
Fucosyltransferase 6/FUT6 |
Generates sLex antigen | |
| Fucosyltransferase 7/FUT7 | Adds (terminal) Fucose to GlcNAc | Generates sLex antigen |
| Fucosyltransferase 8/FUT8 | Adds (non-terminal) Fucose to GlcNAc | |
|
Fucosyltransferase 9/FUT9 |
Generates sLex antigen | |
| Fucosyltransferase 11/FUT11 | Adds (non-terminal) Fucose to GlcNAc |
- alpha-L-Fucosidase
- Tissue alpha-L-Fucosidase/FUCA1
- Streptavidin-HRP
Schematic
|
The terminal fucose can be removed using a Fucosidase. Azido-fucose can then be added to open sites with specific fucosyltransferases and detected using Biotinylated Alkyne in a click chemistry reaction. |
Sample Data
|
Detecting Open Fucosylation Sites on the Acceptor Substrate Recombinant Drosophila Notch EGF20. rhPOFUT1 at 0.5 µg of was incubated at 37 °C for one hour with increasing amounts (0.5, 1, 2, 4 µg) of rdNotch EGF20 repeat. The left side is a labeling reaction including GDP-Azido-Fucose. The right side serves as a negative control labeling reaction without GDP-Azido-Fucose. The results clearly show GDP-Azido-Fucose incorporation into the Notch EGF20 substrate on open sites for POFUT1-mediated fucosylation. The buffer used contained 25 mM HEPES pH 7.5, 150 mM NaCl, and 10 mM MnCl2 for a total volume of 40 µl. The reactions were then conjugated for one hour with 1 nmol of Biotinylated Alkyne in the prescence of 100 µM CuCl2 and 2 mM Ascorbic Acid for a final volume of 50 µl. All reactions were separated on 12% SDS-PAGE and then detected with Streptavidin-HRP. |
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at < -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Good for 12 months from date of receipt.
Product Documents for GDP-Azido-Fucose
Product Specific Notices for GDP-Azido-Fucose
For research use only
Loading...
Loading...
Loading...
Loading...