Skip to main content


Bio-Techne includes R&D Systems | Catalog # ES101

Activated Sugar

Key Product Details

Key Benefits:

Learn more about Fluorescent Glycan Labeling and Detection


Assay Procedure

Sample Protocol for Core-6 Fucose Labeling

Protocols are guidelines. Parameters need to be optimized by end users.



Assay Procedure

1. Prepare a reaction mixture by combining 5 µg of Protein Sample, 1 µg of rhFUT8 in the presence of 1 nmol GDP-Azido-Fucose in the Assay Buffer with the final volume of 25 µL.


2. Prepare negative controls according to step 1 but omit Protein Sample or rhFUT8 or GDP-Azido-Fucose.


3. Incubate all the reactions and the controls at 37°C for one hour.


4. To each of the samples, add 5 µL of 1 mM CuCl2, 5 µL of 20 mM Ascorbic Acid, and 5 µL of 1 mM Biotinylated Alkyne. Mix with gentle tapping.


5. Incubate all samples at room temperature for 1 hour.


6. Separate the reactions and controls by 12% SDS-PAGE.


7. Blot the gel to a nitrocellulose membrane.


8. Block the blot with 10% fat-free milk for 5 minutes.


9. Thoroughly wash the membrane with TBST buffer by changing buffer three times for a total of 45 minutes.


10. Incubate the blot with 25 ng/mL Streptavidin-HRP in 30 mL TBST buffer for 30 minutes.


11. Thoroughly wash the membrane with TBST buffer by changing buffer three times for a total of 45 minutes.


12. Detect with commercial ECL (Enhanced Chemiluminescence) reagents.

Final Assay Conditions Per Reaction

  • GDP-Azido-Fucose: 1 nmol
  • rhFUT8: 1 µg
  • Protein Sample: 5 µg
  • Reaction volume: 25 µl

Click Chemistry Reaction Conditions Per Reaction

  • CuCl2: 5 nmol
  • Ascorbic Acid: 100 nmol
  • Biotinylated Alkyne: 5 nmol
  • Reaction volume: 40 µl

Citations for GDP-Azido-Fucose (4)

Showing  1 - 4 of 4 citations Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for GDP-Azido-Fucose include:

There are no citations that match your criteria.

Showing  1 - 4 of 4 citations Showing All

Customer Reviews for GDP-Azido-Fucose

There are currently no reviews for this product. Be the first to review GDP-Azido-Fucose and earn rewards!

Have you used GDP-Azido-Fucose?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥1250 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Product Documents for GDP-Azido-Fucose

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.