Biological Activity for TSA Vivid™ Fluorophore Kit 650
TSA Vivid™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. TSA Vivid reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.
The basis for signal amplification comes from the specific and high-density deposition of TSA Vivid dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).
Excitation maximum = 654 nm; emission maximum = 668 nm
Please see the product datasheet or protocol for information on product use.
TSA Vivid is a trademark of Bio-Techne Corp.
Scientific Data Examples for TSA Vivid™ Fluorophore Kit 650
Application of TSA Vivid dye 650 in RNAscope™ Multiplex Fluorescent v2 Assay.
RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and TSA Vivid dye 650 (right). Dye used at 1:1500 dilution. Marker shown is UBC in white. Nuclei are counterstained with DAPI (blue, Catalog # 5748, Tocris).
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Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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