Human p63/TP73L Antibody

Name: Human p63/TP73L Antibody
Brand: R&D Systems
SKU: AF1916
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1916

Citations (9)

(3)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human p63/TP73L Met40-Cys339 Accession # Q9H3D4
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human p63/TP73L in direct ELISAs and Western blots.

View Full Technical Details for Human p63/TP73L Antibody

Applications for Human p63/TP73L Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human p63/TP73L
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human p63/TP73L Antibody

	Immunohistochemistry p63/TP73L in Human Breast. p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunocytochemistry p63/TP73L in SCC-25 Human Cell Line. p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: p63/TP73L

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

Item	Value
References	Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.
Long Name	Tumor Protein 63
Entrez Gene IDs	8626 (Human)
Alternate Names	CUSP, Chronic ulcerative stomatitis protein, EEC3, EEC3B(p51B), KET, KETRHS, Keratinocyte transcription factor KET, LMS, OFC8, OFC8SHFM4TP73LNBP, SHFM4, TP53CP, TP53L, TP63, TP73L, Transformation-related protein 63, Tumor protein p73-like, amplified in squamous cell carcinoma, p40, p51, p51tumor protein p53-like, p53CP, p63, p63AIS, p73H, p73L, p73LB(p51A), tumor protein 63, tumor protein p53-competing protein, tumor protein p63, tumor protein p63 deltaN isoform delta

Citations for Human p63/TP73L Antibody (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human p63/TP73L Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

Z Zhang et al. (2022), Differential susceptibility to SARS-CoV-2 in the normal nasal mucosa and in chronic sinusitis European Journal of Immunology, 0(0).
PMID: 35524548
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

MG Dalghi et al. (2021), Functional roles for Piezo1 and Piezo2 in urothelial mechanotransduction and lower urinary tract interoception JCI Insight, 0(0).
PMID: 34464353
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Y Liu et al. (2020), Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma Cell Rep, 30(3):771-782.e6.
PMID: 31968252
Species: Human
Sample Types: Whole Tissue
Applications: IHC

J Ancel et al. (2020), Sonic hedgehog signalling as a potential endobronchial biomarker in COPD Respir. Res., 21(1):207.
PMID: 32767976
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Z Diabasana et al. (2020), Nicotinic Receptor Subunits Atlas in the Adult Human Lung Int J Mol Sci, 21(20).
PMID: 33050277
Species: Human
Sample Types: Whole Tissue
Applications: IHC

J Ancel et al. (2020), Sonic hedgehog signalling as a potential endobronchial biomarker in COPD Respir. Res., 21(1):207.
PMID: 32767976
Species: Human
Sample Types: Whole Tissue
Applications: IHC

R Belgacemi et al. (2019), Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine, 51(0):102572.
PMID: 31877414
Species: Human
Sample Types: Whole Tissue
Applications: IHC

B You et al. (2019), The oncogenic and druggable hPG80 (Progastrin) is overexpressed in multiple cancers and detected in the blood of patients EBioMedicine, 51(0):102574.
PMID: 31877416
Species: Human
Sample Types: Cell Lysates
Applications: ChIP

JS Beeler et al. (2019), p73 regulates epidermal wound healing and induced keratinocyte programming PLoS ONE, 14(6):e0218458.
PMID: 31216312

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Customer Reviews for Human p63/TP73L Antibody (3)

4 out of 5

3 customer ratings

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Human p63/TP73L Antibody

Name: Michele Torino

Application: WB

Sample Tested: 293T human embryonic kidney cell line

Species: Human

Verified Customer | Posted 12/07/2017
Human p63/TP73L Antibody

Name: Anonymous

Application: WB

Sample Tested: Mammary gland tissue

Species: Human

Verified Customer | Posted 05/16/2017
Human p63/TP73L Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Prostate cancer

Species: Human

Verified Customer | Posted 12/15/2016

p63 is prostate stem cell marker. i have isolated stem cells from BPH patients and stained with p63 marker. I found excellent results with DAB kit and also p63 antibody performed superb job.

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Product Documents for Human p63/TP73L Antibody

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Human p63/TP73L Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human p63/TP73L Antibody

Scientific Data Examples for Human p63/TP73L Antibody

Immunohistochemistry

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: p63/TP73L

Citations for Human p63/TP73L Antibody (9)

Customer Reviews for Human p63/TP73L Antibody (3)

Human p63/TP73L Antibody

Human p63/TP73L Antibody

Human p63/TP73L Antibody

Product Documents for Human p63/TP73L Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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