p63/TP73L was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

p63/TP73L in SCC-25 Human Cell Line.

p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human p63/TP73L Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SCC-25 human tongue carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate array

Western Blot

0.1 µg/mL
Sample: Recombinant Human p63/TP73L

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p63/TP73L

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

References

Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
Benard, J. et al. (2003) Hum. Mutat. 21(3):182.

Long Name

Tumor Protein 63

Alternate Names

EEC3, KET, OFC8, p40, p51, p73L, SHFM4, TP63, TP73L

Entrez Gene IDs

8626 (Human)

Gene Symbol

TP63

UniProt

Q9H3D4

Additional p63/TP73L Products

Product Documents for Human p63/TP73L Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human p63/TP73L Biotinylated Antibody

For research use only

Citations
Reviews

Human p63/TP73L Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1916

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human p63/TP73L Biotinylated Antibody

p63/TP73L in Human Prostate.