Human/Mouse E-Cadherin Antibody

Name: Human/Mouse E-Cadherin Antibody
Brand: rnd
SKU: AF748
Rating: 4 (7 reviews)

Catalog # AF748

Citations (37)

(7)

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Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin Asp157-Val709 Accession # P09803
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse E-Cadherin in direct ELISAs and Western blots.

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Applications

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Flow Cytometry	0.25 µg/10⁶ cells	D3 mouse embryonic stem cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human/Mouse E-Cadherin Antibody Scientific Data Examples

	Western Blot Detection of Human and Mouse E‑Cadherin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry E‑Cadherin in Mouse Skin. E-Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
	Immunocytochemistry E‑Cadherin in D3 Mouse Embryonic Stem Cell Line. E-Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
	Immunocytochemistry E‑Cadherin in Mouse Intestinal Organoids. E-Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and counterstained with DAPI (blue). Magnification shown at 100X (upper panel) and 40X (lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunocytochemistry E‑Cadherin in Mouse Intestinal Organoids. E-Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry E‑Cadherin in Mouse Spinal Cord. E-Cadherin was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Simple Western Detection of Human and Mouse E‑Cadherin by Simple Western^TM. Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E-Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: E-Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

Item	Value
References	Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123. Overduin, M. et al. (1995) Science 267:386. Takeichi, M. (1991) 251:1451.
Entrez Gene IDs	999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names	Arc-1, CAD1, CAM 120/80, CD324, CD324 antigen, CDH1, CDHE, Cadherin-1, Cell-CAM120/80, E-Cadherin, ECAD, ECadherin, Epithelial cadherin, L-CAM, LCAM, UVOE-Cadherin, Uvomorulin, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), calcium-dependent adhesion protein, epithelial, cell-CAM 120/80

Citations (37)

Showing 1 - 10 of 37 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse E-Cadherin Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: Neutralization

Dassah M et al. (2104), Annexin A2 mediates secretion of collagen VI, pulmonary elasticity and apoptosis of bronchial epithelial cells. J Cell Sci, 127(0):828-44.
PMID: 24357721
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

T Ringel et al. (2020), Genome-Scale CRISPR Screening in Human Intestinal Organoids Identifies Drivers of TGF-&beta Resistance Cell Stem Cell, 26(3):431-440.e8.
PMID: 32142663
Species: Mouse
Sample Types: Colon Tissue
Applications: IHC

J Jin et al. (2020), CD137 Signaling Regulates Acute Colitis via RALDH2-Expressing CD11b-CD103+ DCs Cell Rep, 30(12):4124-4136.e5.
PMID: 32209473
Species: Mouse
Sample Types: Cells
Applications: IF

M Reed et al. (2019), Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo Nat Commun, 10(1):5004.
PMID: 31676794
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

AD Werts et al. (2019), A novel role for necroptosis in the pathogenesis of necrotizing enterocolitis Cell Mol Gastroenterol Hepatol, 0(0):.
PMID: 31756560
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

C Liu et al. (2019), Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells Nat Commun, 10(1):4589.
PMID: 31597917
Species: Mouse
Sample Types: Whole Tissue
Applications: Confocal Microscopy

MK Yanda et al. (2019), Pharmacological reversal of renal cysts from secretion to absorption suggests a potential therapeutic strategy for managing polycystic kidney disease J. Biol. Chem., 0(0):.
PMID: 31570523
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

K Nakajima et al. (2019), Lack of whey acidic protein four disulphide core (WFDC) 2 protease inhibitor causes neonatal death from respiratory failure in mice Dis Model Mech, 0(0):.
PMID: 31562139
Species: Mouse
Sample Types: Whole Tissue
Applications: Proximity Ligation Assay (PLA)

DM Gay et al. (2019), Loss of BCL9/9l suppresses Wnt driven tumourigenesis in models that recapitulate human cancer Nat Commun, 10(1):723.
PMID: 30760720
Species: Human
Sample Types: Whole Cells
Applications: ICC

O Nekrasova et al. (2018), Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination Nat Commun, 9(1):1053.
PMID: 29535305
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

DL Coutu et al. (2018), Multicolor quantitative confocal imaging cytometry Nat. Methods, 15(1):39-46.
PMID: 29320487
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

HP Pui et al. (2018), NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes Mech. Dev., 0(0):.
PMID: 29339164
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

J Wang et al. (2018), Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication Cell Rep, 25(2):464-477.e4.
PMID: 30304685
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

S Flemming et al. (2018), Analysis of leukocyte transepithelial migration using an in vivo murine colonic loop model JCI Insight, 3(20):.
PMID: 30333307
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

VP O'Brien et al. (2018), Host restriction of Escherichia coli recurrent urinary tract infection occurs in a bacterial strain-specific manner PLoS Pathog., 14(12):e1007457.
PMID: 30543708
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

MA Scavuzzo et al. (2018), Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA Cell Rep, 25(13):3811-3827.e7.
PMID: 30590051
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

P Treerat et al. (2017), Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection Mucosal Immunol, 0(0):.
PMID: 28247861
J Kim et al. (2017), Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic ? Cell Hyperplasia in Mice Cell Metab., 25(6):1348-1361.e8.
PMID: 28591637
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

A Molotkov et al. (2017), Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency Dev. Cell, 0(0):.
PMID: 28552557
E Sakai et al. (2017), Combined mutation of Apc, Kras and Tgfbr2 effectively drives metastasis of intestinal cancer Cancer Res., 0(0):.
PMID: 29282223
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

(2016), Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling PLoS Genet, 12(11):e1006442.
PMID: 27893742
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

(2016), A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease Nat Microbiol, 2(0):16196.
PMID: 27798558
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Y Kato et al. (2016), Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells Nat Commun, 7(0):11272.
PMID: 27072294
Species: Mouse
Sample Types: Whole Cells
Applications: IHC

Kida Y et al. (2015), EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury. J Am Soc Nephrol, 24(4):559-72.
PMID: 23492730
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Huels D et al. (2015), E-cadherin can limit the transforming properties of activating beta-catenin mutations. EMBO J, 34(18):2321-33.
PMID: 26240067
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Ihermann-Hella A et al. (2014), Mitogen-activated protein kinase (MAPK) pathway regulates branching by remodeling epithelial cell adhesion. PLoS Genet, 10(3):e1004193.
PMID: 24603431
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Grabinski N et al. (2014), AKT3 regulates ErbB2, ErbB3 and estrogen receptor alpha expression and contributes to endocrine therapy resistance of ErbB2(+) breast tumor cells from Balb-neuT mice. Cell Signal, 26(5):1021-9.
PMID: 24463007
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Guan X et al. (2013), MMP20 modulates cadherin expression in ameloblasts as enamel develops. , 92(0):.
PMID: 24067343
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Miyauchi et al. (2013), Bifidobacterium longum alleviates dextran sulfate sodium-induced colitis by suppressing IL-17A response: involvement of intestinal epithelial costimulatory molecules. PLoS ONE, 8(11):e79735.
PMID: 24255712
Species: Human
Sample Types: Whole Cells
Applications: IHC

Neal M et al. (2013), A critical role for TLR4 induction of autophagy in the regulation of enterocyte migration and the pathogenesis of necrotizing enterocolitis. J Immunol, 190(7):3541-51.
PMID: 23455503
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Obata Y et al. (2012), Epithelial cell-intrinsic notch signaling plays an essential role in the maintenance of gut immune homeostasis. J. Immunol., 188(5):2427-36.
PMID: 22279105
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012), N-cadherin+ HSCs in fetal liver exhibit higher long-term bone marrow reconstitution activity than N-cadherin- HSCs. Biochem Biophys Res Commun, 428(3):354-9.
PMID: 23092738
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Betterman K et al. (2012), Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli. Am J Pathol, 181(6):2225-38.
PMID: 23063660
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Wang H et al. (2011), Noninvasive assessment of antenatal hydronephrosis in mice reveals a critical role for Robo2 in maintaining anti-reflux mechanism. PLoS ONE, 6(9):e24763.
PMID: 21949750
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Nakahara T et al. (2006), Effect of inhibition of vascular endothelial growth factor signaling on distribution of extravasated antibodies in tumors. Cancer Res., 66(3):1434-45.
PMID: 16452199
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Matsuo S et al. (2005), Multifunctionality of PAI-1 in fibrogenesis: evidence from obstructive nephropathy in PAI-1-overexpressing mice. Kidney Int., 67(6):2221-38.
PMID: 15882265
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Grieshammer U et al. (2005), FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons. Development, 132(17):3847-57.
PMID: 16049112

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Customer Reviews (7)

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7 customer ratings

5 stars

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Showing 1 - 5 of 7 reviews Showing All

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Human/Mouse E-Cadherin Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Embryonic lung

Species: Mouse

Verified Customer | Posted 03/21/2019
Human/Mouse E-Cadherin Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: RT4-D6P2T rat schwannoma cell line

Species: Human

Verified Customer | Posted 01/14/2019
Human/Mouse E-Cadherin Antibody

Name: Anonymous

Application: IHC

Sample Tested: Tumor cell lyastes

Species: Mouse

Verified Customer | Posted 04/13/2018
Human/Mouse E-Cadherin Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Bladder cancer tissue

Species: Human

Verified Customer | Posted 12/19/2017
Human/Mouse E-Cadherin Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: iPSC derived epithelial cells

Species: Human

Verified Customer | Posted 09/27/2017
Human/Mouse E-Cadherin Antibody

Name: Anonymous

Application: WB

Sample Tested: 293T human embryonic kidney cell line

Species: Human

Verified Customer | Posted 07/31/2017
Human/Mouse E‑Cadherin Antibody

Name: Anonymous

Application: WB

Sample Tested: mouse primary brain cell cultures

Species: Mouse

Verified Customer | Posted 04/21/2016

Ab concentration: 0.1 ug/ml

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Showing 1 - 5 of 7 reviews Showing All

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Human/Mouse E-Cadherin Antibody

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Key Product Details

Complete Your Research

Complete Your Experiment

Applications

Human/Mouse E-Cadherin Antibody Scientific Data Examples

Western Blot

Immunohistochemistry

Immunocytochemistry

Immunocytochemistry

Immunocytochemistry

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: E-Cadherin

Citations (37)

Customer Reviews (7)

Customer Images

Human/Mouse E-Cadherin Antibody

Human/Mouse E-Cadherin Antibody

Human/Mouse E-Cadherin Antibody

Human/Mouse E-Cadherin Antibody

Human/Mouse E-Cadherin Antibody

Human/Mouse E-Cadherin Antibody

Human/Mouse E‑Cadherin Antibody

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Product Datasheets

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