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Cultrex Organoid Harvesting Solution

Ready-to-use, non-enzymatic organoid harvesting and dissociation solution

Key Product Details

Features: Ready-to-use, non-enzymatic solution to depolymerize basement membrane matrix for harvesting organoids from culture.
Cultrex Organoid Harvesting Solution

Assay Procedure

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FAQs

  • Can the Organoid Harvesting Solution, Catalog # 3700-100-01,  be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?

    The Organoid Harvesting Solution works well with BME (Basement Membrane Extract).  For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.

  • Are Cultrex Organoid Harvesting Solution (Catalog # 3700-100-01) and dispase interchangeable?

    Cultrex Organoid Harvesting Solution (Catalog # 3700-100-01) is a non-enzymatic solution that depolymerizes the matrix to enable harvest of organoids. In contrast, dispase is a protease enzyme that degrades the matrix. End users will need to empirically determine whether enzymatic or non-enzymatic solutions are more appropriate for their experimental setup.

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