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Scientists' Comments About Our Organoid-Related Workflow Solutions

Photograph of Jens Pushof from the Hans Clevers lab at the Hubrecht Institute in The Netherlands

“Our research is greatly facilitated by Bio-Techne products. The various organoid systems and co-cultures are all performed in or on R&D Systems Cultrex Reduced Growth Factor BME with great results. Even for more exotic organoids, such as snake venom gland organoids, we could achieve breakthroughs with R&D Systems growth factors and BME as well as spatial visualization of toxin transcripts using ACD’s RNAScope.”

Jens Puschhof | Hans Clevers Lab | Hubrecht Institute, The Netherlands   

“We used a range of products from Bio-Techne in this study. iPSCs were directed to form human intestinal organoids using noggin, EGF and CHIR99021 from R&D Systems and Tocris and these consistently generated high quality organoids. Despite limited experience with in situ hybridization, we were able to visualize LGR5 and WDR43 with relative ease using probes and reagents from ACD.”

Dr. Robert Barrett | Cedars-Sinai Medical Center, CA, USA   

Photograph of Dr. Robert Barrett from Cedars-Sinai Medical Center in CA, USA
Photograph of Dr. Tayla Dayton from the Hans Clevers lab at the Hubrecht Institute in The Netherlands

“One of the key features of organoids in general is the defined media that we use. This allows us to tailor the in vitro niche environment to the specific cells we are growing or to the cells that we are trying to generate in culture. This has been made possible, in part, through the use of a wide range of different growth factors and small molecules from R&D Systems and Tocris. We also used Cultrex for all of the organoids in this study.”

Dr. Tayla Dayton | Hans Clevers Lab | Hubrecht Institute, The Netherlands 

“We utilize organoids as powerful research tools to study the underlying regulation of stem cells and cancer and to develop innovative strategies for regenerative medicine. We cultured these 3D organoids in the lab using the R&D Systems CultrexTM Reduced Growth Factor BME, which gave very consistent and reliable results. Other R&D Systems reagents such as growth factors and ACD’s RNAScopeTM technology have all contributed significantly to our findings.”

Dr. Vivian Li | Crick Institute, London, UK

Dr Vivian Li 525
Photograph of Sunita D’Souza, Director of the Center for Modern Pediatric Disease at St. Jude’s Medical Center

“I have been using R&D/ Bio-Techne products for the last, maybe, 20 years. So ever since I was a graduate student, my lab would exclusively use R&D products. I've found R&D/ Bio-Techne to be extremely reliable.”

Sunita D'Souza | Director of the Center for Modern Pediatric Diseases | St. Jude's Medical Center

Scientists' Comments about Our Cytokines and Growth Factors

Photograph of Deborah French, Director of the Human Pluripotent Stem Cell Core at the Children’s Hospital of Pennsylvania

“The Bio-Techne products that we're using, we love, and what we're using are cytokines, growth factors and we have been using them since the beginning. I mean, our core started in 2008 and we really have not changed. The product, the reproducibility has been consistent, and we've seen that everything works, everything is good and now so a lot of the products we buy and we don't lot test and we haven't had issues. Everything has been excellent and has worked beautifully.”

Deborah French| Director of the Human Pluripotent Stem Cell Core | Children's Hospital of Pennsylvania

“We had very good experience with R&D cytokines as well as antibodies. We didn't find any lot-to-lot variation with the cytokines and antibodies so, in general, we're very pleased.”

Wenli Yang | Director of the iPSC Core Facility | University of Pennsylvania

Photograph of Wenli Yang, Director of the iPSC Core Facility at the University of Pennsylvania
Photograph of Barbara Corneo, Director of the Stem Cell Core Facility at Columbia University Medical School

So in our stem cell core facility, we do a lot of differentiation into different progenies. For all this differentiation we use a number of different cytokines, growth factors and we buy them all from R&D Systems or Tocris.”

Barbara Corneo | Director of the Stem Cell Core Facility | Columbia University Medical School

Scientists' Comments About Our Cultrex BME Products

Photograph of Ken Diffenderfer, Director of the Stem Cell Core Facility at the Salk Institute in La Jolla, CA, USA

“In 2015, we started to evaluate Cultrex BME as an alternative to Matrigel®. At that time, we pursued some rigorous quality control testing comparing Cultrex to Matrigel. And in that, we tested 10 cell lines for over 20 passages through various functional assays, and found it to be an incredibly stable and consistent product. Since that time, we have been lot testing Cultrex BME for individual lot purchases, as we consume that reagent. And we have tested approximately 20 lots of Cultrex BME in that time. Through those 20 lots, not a single one has failed our internal QC paradigm.”

Ken Diffenderfer | Director of the Stem Cell Core Facility | Salk Institute for Biological Studies, CA, USA

Matrigel is a registered trademark of Corning Life Sciences.

“We used Cultrex BME as the matrix for our experiments to support organoid culture with great consistent results. The ACE-2 R&D Systems antibody really helped in our work to assess the expression profile of this receptor in intestinal organoids, and to follow changes upon SARS-CoV2 infection.”

Joep Beumer | Hans Clevers Lab | Hubrecht Institute, The Netherlands

Photograph of Joep Beumer from the Hans Clevers lab at the Hubrecht Institute in The Netherlands

“We found that UltiMatrix could be used for various purposes easily, and being able to modify rigidity of the gel by diluting with medium was especially convenient. When culturing colon and pancreas organoids with UltiMatrix, we couldn’t quantify differences compared to Matrigel® BME. In our hands, the organoids grew equally well. However, overall, we could observe very healthy and well-structured 3D morphology of the organoids in UltiMatrix, and found slightly bigger organoids in UltiMatrix gel than the others. Our overall impression of UltiMatrix is that it can be a very convenient and efficient matrix especially if the dilution factor is optimized depending on organoid type."

Dr. Sangho Lim | Hans Clevers Lab | Hubrecht Institute, The Netherlands

Matrigel is a registered trademark of Corning Life Sciences.

“Ultimatrix is unique in its ability to bead up to form a droplet at even low concentrations. We have found that preparing protein concentrations below 8 mg/mL for other commercial ECMs causes 3-dimensional tissues, such as intestinal organoids, to sink to the plastic leading to differentiation and cell death, while UltiMatrix supports healthy normal growth.”

Michael K. Dame | Associate Director of the Translational Tissue Modeling Institute | Jason Spence Laboratory | University of Michigan, MI, USA

I have used Bio-Techne Ultimatrix for organoïds research and the results are comparable to the Corning Matrigel® in terms of growth, 3D structure and biomarkers expression. Ultimatrix is really convenient to use, has lower endotoxin, is reproducible lot to lot and is considerably better priced. I fully recommend the use of Ultimatrix for organoïds research."

Organoids Section Team Leader | Drug Discovery | Paris, France

Matrigel is a registered trademark of Corning Life Sciences.

“Cultrex ReadyBME has worked very well for our lab. H9 hESCs (WA09) were grown on Cultrex ReadyBME and was directly compared side-by-side to our normal basement membrane. So far, I’ve grown the cells for more than 5 passages and haven’t observed any changes in proliferation rate, viability, cell morphology or the increased appearance of differentiated cells.”

Dr. Amar M. Singh | University of Georgia

Further Reviews from Our Protein Product Pages

Recombinant Human/Mouse/Rat Activin A (R&D Systems, Catalog # 338-AC)

Avg 4.7/5 stars, 12 reviews

Application: Stem/Immune cell maintenance or differentiation

“This reagent worked perfectly for the maintenance of trophoblast stem cells.”

“Routinely used in our lab for growth-factor guided differentiation protocol for cardiomyocyte differentiation from human pluripotent stem cells.”

Recombinant Human R-Spondin 1 (R&D Systems, Catalog # 4645-RS)

Avg: 4.5/5 stars, 4 reviews

Application: Stem/Immune cell maintenance or differentiation

“Stable product when used as described; produces repeatable results for differentiation.”

Recombinant Human Wnt-3a (R&D Systems, Catalog # 5036-WN)

Avg: 4.6/5 stars, 17 reviews

Application: In vitro bioactivity in cell culture

“This protein (200 ng/mL) works well for activating Wnt signaling as shown by Topflash luciferase activity.”

“I have used Wnt-3a routinely to stimulate primary cells in culture. I have obtained consistent results with reproducible data.”

Application: Media additive for protein or antibody production

“This protein works very well for activating Wnt signaling as shown by beta-Catenin expression.”

Recombinant Human/Mouse Rat BMP-2 (R&D Systems, Catalog # 355-BM)

Avg: 4.8/5 stars, 9 reviews

Application: In vitro bioactivity in cell culture

“Another great product made by R&D Systems. Always produces consistent and reliable results. Great as a control.”

Recombinant Human BMP-4 (R&D Systems, Catalog # 314-BP)

Avg: 4.9/5 stars, 8 reviews

Application: Stem/Immune cell maintenance or differentiation

“Robust response in our differentiation; stable and repeatable results when used as described.”

Application: In vitro bioactivity in cell culture

“The product worked really well and it was easy to handle.”

Recombinant Human EGF (R&D Systems, Catalog # 236-EG)

Avg: 4.8/5 stars, 21 reviews

Application: Stem/Immune cell maintenance or differentiation

“Performed well on differentiating iPSCs into neurons.”

Tested with the same application on different species and the result is always excellent.”

Recombinant Human FGF basic/FGF2 (R&D Systems, Catalog # 233-FB)

Avg: 4.7/5 stars, 17 reviews

Application: In vitro bioactivity in cell culture

“This bFGF worked well in supporting the growth of LUHMES cells and maintaining the cells in an undifferentiated state.”

Application: Stem/Immune cell maintenance or differentiation

“Recombinant protein used to induce spheres from olfactory stem cells and the result was always excellent.”

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