Recombinant Human R-Spondin 1 Protein Best Seller
Catalog # 4645-RS | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Ser21-Ala263
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Scientific Data Images for Recombinant Human R-Spondin 1 Protein
Recombinant Human R-Spondin 1 Protein Bioactivity
Recombinant Human R-Spondin 1 (Catalog # 4645-RS), in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN); 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay (blue). The activity is approximately 7-fold greater than the competitor's R-Spondin 1 (red).Recombinant Human R-Spondin 1 Protein Lot-to-Lot Consistency
The lot-to-lot consistency of Recombinant Human R-Spondin 1 (Catalog # 4645-RS) was assessed by testing the ability of three independent lots of the protein to stimulate activation of beta-Catenin using a TOPflash beta-Catenin/TCF reporter assay in the HEK293T human kidney cell line, in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (1324-WN). Each trace on the graph represents data obtained from Recombinant Human R-Spondin 1 from a different manufacturing run. The ED50 for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.Recombinant Human R-Spondin 1 Protein SDS-PAGE
1 µg/lane of Recombinant Human R-Spondin 1 was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 39 kDa.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 4645-RS
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 4645-RS/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: R-Spondin 1
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).
Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively (14). Over aa 21-263, human R-Spondin 1 shares 89%, 87%, 92%, 91%, 91% and 89% aa identity with mouse, rat, horse, dog, goat, and cow RSPO-1, respectively.
References
- Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
- Kim, K.-A. et al. (2006) Cell Cycle 5:23.
- Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
- Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
- Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
- Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
- Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
- Parma, P. et al. (2006) Nat. Genet. 38:1304.
- Kim, K.-A. et al. (2005) Science 309:1256.
- Zhao, J. et al. (2007) Gastroenterology 132:1331.
- Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
- Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
- Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
- UniProt # Q2MKA7.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human R-Spondin 1 Protein
Product Specific Notices for Recombinant Human R-Spondin 1 Protein
For research use only
Citations for Recombinant Human R-Spondin 1 Protein (97)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human R-Spondin 1 Protein include:
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Species: Mouse
Sample Types: Organoid
Applications: Cell CultureBuñay et al. (2023-08-17), Persistent organic pollutants promote aggressiveness in prostate cancer Oncogene
PMID: 37587334 -
Species: Human
Sample Types: Organoids
Applications: BioassaySuzuki et al. (2023-08-01), Genetic characterization of a novel organoid from human malignant giant-cell tumor Journal of bone oncology
PMID: 37260767 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayMorral et al. (2023-04-28), p53 promotes revival stem cells in the regenerating intestine after severe radiation injury bioRxiv : the preprint server for biology
PMID: 37162959 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Nomura et al. (2023-02-16), Simple Detection and Culture of Circulating Tumor Cells from Colorectal Cancer Patients Using Poly(2-Methoxyethyl Acrylate)-Coated Plates International Journal of Molecular Sciences, 2023-024(4).
PMID: 36835361 -
Species: Human
Sample Types: Organoids
Applications: BioassayJ Tian et al. (2023-01-26), Combined PD-1, BRAF and MEK inhibition in BRAFV600E colorectal cancer: a phase 2 trial Nature Medicine, 2023-00(0).
PMID: 36702949 -
Species: Human
Sample Types: Organoids
Applications: BioassayY Sun et al. (2023-01-12), Targeting TBK1 to overcome resistance to cancer immunotherapy Nature, 2023-00(0).
PMID: 36634707 -
Species: Human
Sample Types: Organoids
Applications: BioassayY Niu et al. (2022-12-05), Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select Nature Genetics, 2022-154(12):1983-1993.
PMID: 36471068 -
Species: Human
Sample Types: Organoids
Applications: BioassayM Matsumoto et al. (2022-11-26), Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids Micromachines, 2022-113(12).
PMID: 36557386 -
Species: Feline
Sample Types: Organoids
Applications: BioassayRE Thompson et al. (2022-11-17), Generation and cryopreservation of feline oviductal organoids Theriogenology, 2022-1196(0):167-173.
PMID: 36423511 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayY Li et al. (2022-09-07), Identification of trypsin-degrading commensals in the large intestine Nature, 2022-0609(7927):582-589.
PMID: 36071157 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayRE Niec et al. (2022-06-20), Lymphatics act as a signaling hub to regulate intestinal stem cell activity Cell Stem Cell, 2022-029(7):1067-1082.e18.
PMID: 35728595 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Kim et al. (2022-03-30), Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids Nature Communications, 2022-013(1):1692.
PMID: 35354790 -
Species: Human
Sample Types: Organoid
Applications: Cell CultureM Wang et al. (2022-03-23), Renalase and its receptor, PMCA4b, are expressed in the placenta throughout the human gestation Scientific Reports, 2022-012(1):4953.
PMID: 35322081 -
Species: Human
Sample Types: Transduced Whole Cells
Applications: BioassayJ Jeong et al. (2022-03-23), Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids Biology, 2022-011(4).
PMID: 35453693 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassayT Higa et al. (2022-03-21), Spatiotemporal reprogramming of differentiated cells underlies regeneration and neoplasia in the intestinal epithelium Nature Communications, 2022-013(1):1500.
PMID: 35314700 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureKM Sutton et al. (2022-03-02), Establishment of bovine 3D enteroid-derived 2D monolayers Veterinary research, 2022-053(1):15.
PMID: 35236416 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayJA Rappaport et al. (2021-12-22), A beta-Catenin-TCF-Sensitive Locus Control Region Mediates GUCY2C Ligand Loss in Colorectal Cancer Cellular and Molecular Gastroenterology and Hepatology, 2021-10(0).
PMID: 34954189 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayA Gross et al. (2021-12-17), Desmoplakin maintains transcellular keratin scaffolding and protects from intestinal injury Cellular and Molecular Gastroenterology and Hepatology, 2021-10(0).
PMID: 34929421 -
Species: Chicken
Sample Types: Whole Cells
Applications: BioassayB Orr et al. (2021-11-24), Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer Veterinary research, 2021-152(1):142.
PMID: 34819162 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayS Heino et al. (2021-11-17), Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas Science Advances, 2021-17(47):eabj0512.
PMID: 34788095 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayS Lee et al. (2021-09-14), Intestinal antiviral signaling is controlled by autophagy gene Epg5 independent of the microbiota Autophagy, 2021-00(0):1-16.
PMID: 34520306 -
Species: Human
Sample Types: Organoid
Applications: BioassayBM Larsen et al. (2021-07-27), A pan-cancer organoid platform for precision medicine Cell Reports, 2021-036(4):109429.
PMID: 34320344 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayMDA Norman et al. (2021-04-14), Measuring the elastic modulus of soft culture surfaces and three-dimensional hydrogels using atomic force microscopy Nature Protocols, 2021-00(0).
PMID: 33854255 -
Species: Human
Sample Types: Organoids
Applications: BioassayS Io et al. (2021-04-07), Capturing human trophoblast development with naive pluripotent stem cells in�vitro Cell Stem Cell, 2021-028(6):1023-1039.e13.
PMID: 33831365 -
Species: Human
Sample Types: Organoid
Applications: BioassayT Okamoto et al. (2021-03-11), Comparative Analysis of Patient-Matched PDOs Revealed a Reduction in OLFM4-Associated Clusters in Metastatic Lesions in Colorectal Cancer Stem Cell Reports, 2021-00(0).
PMID: 33711267 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Akbari et al. (2021-03-06), LGR5/R-Spo1/Wnt3a axis promotes stemness and aggressive phenotype in hepatoblast-like hepatocellular carcinoma cell lines Cellular Signalling, 2021-00(0):109972.
PMID: 33684507 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayH Ingle et al. (2021-03-05), Murine astrovirus tropism for goblet cells and enterocytes facilitates an IFN-&lambda response in vivo and in enteroid cultures Mucosal Immunology, 2021-00(0).
PMID: 33674763 -
Species: Human
Sample Types: Organoid
Applications: BioassayK Husain et al. (2021-01-26), Farnesyl dimethyl chromanol targets colon cancer stem cells and prevents colorectal cancer metastasis Scientific Reports, 2021-011(1):2185.
PMID: 33500430 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJCM Chan et al. (2021-01-21), Targeted Profiling of Immunological Genes during Norovirus Replication in Human Intestinal Enteroids Viruses, 2021-013(2).
PMID: 33494515 -
Species: Human
Sample Types: Organoid
Applications: BioassaySK Sarvestani et al. (2021-01-11), Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 2021-012(1):262.
PMID: 33431859 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayK Lloyd et al. (2020-11-27), Using systems medicine to identify a therapeutic agent with potential for repurposing in inflammatory bowel disease Dis Model Mech, 2020-10(0).
PMID: 32958515 -
Species: Human
Sample Types: Organoid
Applications: BioassayA Di Grazia et al. (2020-10-19), The Fragile X Mental Retardation Protein regulates RIP1K and colorectal cancer resistance to necroptosis Cell Mol Gastroenterol Hepatol, 2020-10(0).
PMID: 33091622 -
Species: Human
Sample Types: Organoid
Applications: Cell CultureK Dunbar et al. (2020-09-22), Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1 Cell Mol Gastroenterol Hepatol, 2020-00(0).
PMID: 32971322 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayD Chakravart et al. (2020-09-21), Telomere dysfunction activates YAP1 to drive tissue inflammation Nat Commun, 2020-011(1):4766.
PMID: 32958778 -
Species: Human
Sample Types: Organoid
Applications: BioassayRT Zwiggelaar et al. (2020-09-11), LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium Science Advances, 2020-06(37).
PMID: 32917713 -
Species: Human
Sample Types: Organoid
Applications: BioassayMA Sheridan et al. (2020-09-09), Establishment and differentiation of long-term trophoblast organoid cultures from the human placenta Nat Protoc, 2020-00(0).
PMID: 32908314 -
Species: Human
Sample Types: Cell Lysates,Organoid
Applications: Cell Culture,BioassayZ Dantes et al. (2020-08-06), Implementing cell-free DNA of pancreatic cancer patient-derived organoids for personalized oncology JCI Insight, 2020-05(15).
PMID: 32614802 -
Species: Human
Sample Types: Organoids
Applications: BioassaySI Mae et al. (2020-07-28), Expansion of Human iPSC-Derived Ureteric Bud Organoids with Repeated Branching Potential Cell Rep, 2020-032(4):107963.
PMID: 32726627 -
Species: Human
Sample Types: Organoid
Applications: BioassayY Nanki et al. (2020-07-28), Patient-derived ovarian cancer organoids capture the genomic profiles of primary tumours applicable for drug sensitivity and resistance testing Sci Rep, 2020-010(1):12581.
PMID: 32724113 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Xu et al. (2020-06-17), Understanding the regulatory mechanisms of endometrial cells on activities of endometrial mesenchymal stem-like cells during menstruation Stem Cell Res Ther, 2020-011(1):239.
PMID: 32552749 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Yoshida et al. (2020-04-06), Generation of intestinal organoids derived from human pluripotent stem cells for drug testing Sci Rep, 2020-010(1):5989.
PMID: 32249832 -
Species: Human
Sample Types: Whole Cells
Applications: IHC ControlM Zhang et al. (2020-03-11), Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs PLoS ONE, 2020-015(3):e0229445.
PMID: 32160239 -
Species: Human
Sample Types: Whole Tissue
Applications: Tissue CultureM Dossena et al. (2020-03-04), Standardized GMP-compliant scalable production of human pancreas organoids Stem Cell Res Ther, 2020-011(1):94.
PMID: 32127043 -
Species: Human
Sample Types: cell culture
Applications: Cell CultureNJ Hogrebe et al. (2020-02-24), Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020-00(0).
PMID: 32094658 -
Species: Human
Sample Types: Cells
Applications: Cell CultureY Wang et al. (2020-02-17), HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020-021(3):274-286.
PMID: 32066947 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayG Levin et al. (2020-01-20), Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells BMC Biotechnol., 2020-020(1):5.
PMID: 31959207 -
Species: Human
Sample Types: Whole Tissue
Applications: BioassayA Scott et al. (2020-01-01), Comparison of Surgical and Cadaveric Intestine as a Source of Crypt Culture in Humans Cell Transplant, 2020-029(0):9636897209037.
PMID: 32907378 -
Species: Mouse
Sample Types: Organoid
Applications: BioassaySM Syed et al. (2019-12-26), Endometrial Axin2+ Cells Drive Epithelial Homeostasis, Regeneration, and Cancer following Oncogenic Transformation Cell Stem Cell, 2019-126(1):64-80.e13.
PMID: 31883834 -
Species: Mouse
Sample Types: Organoids
Applications: Cell CulturePG Boone et al. (2019-12-02), A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion Nat Commun, 2019-110(1):5490.
PMID: 31792216 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureS Akbari et al. (2019-09-12), Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling Stem Cell Reports, 2019-013(4):627-641.
PMID: 31522975 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayC Lee et al. (2019-09-02), NOD2 Supports Crypt Survival and Epithelial Regeneration after Radiation-Induced Injury Int J Mol Sci, 2019-020(17).
PMID: 31480799 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureCP Nielsen et al. (2019-07-23), USP9X Deubiquitylates DVL2 to Regulate WNT Pathway Specification Cell Rep, 2019-028(4):1074-1089.e5.
PMID: 31340145 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassayCM Dekaney et al. (2019-07-19), Mist1 Expression is Required for Paneth Cell Maturation Cell Mol Gastroenterol Hepatol, 2019-00(0).
PMID: 31330316 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureK Shik Mun et al. (2019-07-16), Patient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders Nat Commun, 2019-010(1):3124.
PMID: 31311920 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayOC Tysoe et al. (2019-05-20), Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue Nat Protoc, 2019-014(6):1884-1925.
PMID: 31110298 -
Species: Mouse,Human
Sample Types: Whole Cells
Applications: BioassayM Urbischek et al. (2019-04-17), Organoid culture media formulated with growth factors of defined cellular activity Sci Rep, 2019-09(1):6193.
PMID: 30996238 -
Species: Human,Mouse
Sample Types: Organoids
Applications: BioassayHLX Wong et al. (2019-04-15), Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling Nat Commun, 2019-010(1):1745.
PMID: 30988299 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Konagaya et al. (2019-01-24), Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells Sci Rep, 2019-09(1):640.
PMID: 30679498 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayMA Scavuzzo et al. (2018-12-26), Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA Cell Rep, 2018-125(13):3811-3827.e7.
PMID: 30590051 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayJD Barros-Sil et al. (2018-12-18), Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer Cell Rep, 2018-125(12):3504-3518.e6.
PMID: 30566873 -
Species: Human
Sample Types: Organoids
Applications: BioassayR Cruz-Acuña et al. (2018-09-01), PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery Nat Protoc, 2018-00(0).
PMID: 30190557 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayB Zwarycz et al. (2018-07-04), IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model Cell Mol Gastroenterol Hepatol, 2018-07(1):1-17.
PMID: 30364840 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Park et al. (2018-05-11), Differential activities and mechanisms of the four R-Spondins in potentiating Wnt/?-catenin signaling J. Biol. Chem., 2018-00(0).
PMID: 29752411 -
Species: Avian - Chicken
Sample Types: Whole Cells
Applications: BioassayJ Li et al. (2018-05-01), Culture and characterization of chicken small intestinal crypts Poult. Sci., 2018-00(0).
PMID: 29509914 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayYC Chen et al. (2018-04-01), ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA Cell Death Dis, 2018-09(4):437.
PMID: 29740017 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayAM Lebensohn et al. (2018-02-06), R-spondins can potentiate WNT signaling without LGRs Elife, 2018-07(0).
PMID: 29405118 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayNK Lee et al. (2018-01-15), Cell-type specific potent Wnt signaling blockade by bispecific antibody Sci Rep, 2018-08(1):766.
PMID: 29335534 -
Species: Human,Mouse
Sample Types: Whole Cells
Applications: BioassayA Fumagalli et al. (2018-01-04), A surgical orthotopic organoid transplantation approach in mice to visualize and study colorectal cancer progression Nat Protoc, 2018-013(2):235-247.
PMID: 29300390 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayA Taguchi et al. (2017-11-09), Higher-Order Kidney Organogenesis from Pluripotent Stem Cells Cell Stem Cell, 2017-10(0).
PMID: 29129523 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayX Zhou et al. (2017-09-22), R-Spondin1/LGR5 Activates TGF? Signaling and Suppresses Colon Cancer Metastasis Cancer Res., 2017-00(0).
PMID: 28939678 -
Species: Rat
Sample Types: Whole Cells
Applications: BioassayH Isshiki et al. (2017-05-27), Establishment of a refined culture method for rat colon organoids Biochem. Biophys. Res. Commun., 2017-00(0).
PMID: 28559141 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayY Gao et al. (2017-04-26), Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway Stem Cell Res Ther, 2017-08(1):98.
PMID: 28446252 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassayW Chen et al. (2017-02-20), Directional delivery of RSPO1 by mesenchymal stem cells ameliorates radiation-induced intestinal injury Cytokine, 2017-095(0):27-34.
PMID: 28214675 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassaySH Han et al. (2017-02-14), Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor World J. Gastroenterol, 2017-023(6):964-975.
PMID: 28246470 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayL Nigmatulli et al. (2017-01-11), Id2 controls specification of Lgr5+ intestinal stem cell progenitors during gut development EMBO J., 2017-036(7):869-885.
PMID: 28077488 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayLebensohn A et al. (2016-12-20), Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling. Elife, 2016-15(0).
PMID: 27996937 -
Species: Human
Sample Types: Whole Cells
Applications: Bioassay(2016-11-09), PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells Sci Rep, 2016-16(0):36795.
PMID: 27827428 -
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay(2016-09-19), Intestinal enteroids model GUCY2C-dependent secretion induced by heat-stable enterotoxins Infect Immun, 2016-00(0).
PMID: 27481254 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassaySW Huang et al. (2016-08-25), P2X7 receptor-dependent tuning of gut epithelial responses to infection Immunol. Cell Biol, 2016-095(2):178-188.
PMID: 27559003 -
Species: Human
Sample Types: Whole Cells
Applications: Bioassay(2016-07-11), Novel Bi-specific Domain Antibody to LRP6 Inhibits Wnt and R-spondin Ligand-induced Wnt Signaling and Tumor Growth Mol Cancer Res, 2016-00(0).
PMID: 27401612 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayRouch J et al. (2016-01-28), Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids. PLoS ONE, 2016-011(1):e0148216.
PMID: 26820624 -
Species: Mouse
Sample Types: In Vivo
Applications: BioassayChartier C et al. (2015-12-30), Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates beta-Catenin Signaling and Tumorigenesis in Multiple Cancer Types. Cancer Res, 2015-176(3):713-23.
PMID: 26719531 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJames M et al. (2015-02-06), Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note. PLoS ONE, 2015-010(2):e0117776.
PMID: 25658706 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJabaji Z et al. (2014-09-15), Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium. PLoS ONE, 2014-09(9):e107814.
PMID: 25222024 -
Species: Human
Sample Types: Whole Tissue
Applications: BioassaySkoczek D et al. (2014-06-06), Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium. J Immunol, 2014-0193(1):439-51.
PMID: 24907348 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassayYimlamai D et al. (2014-06-05), Hippo pathway activity influences liver cell fate. Cell, 2014-0157(6):1324-38.
PMID: 24906150 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayCarmon K et al. (2014-03-17), RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. Proc Natl Acad Sci U S A, 2014-0111(13):E1221-9.
PMID: 24639526 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayRonaghi et al. (2014-03-10), Inner ear hair cell-like cells from human embryonic stem cells. Stem Cells Dev, 2014-023(11):1275-84.
PMID: 24512547 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGuezguez A et al. (2014-02-15), Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Exp Cell Res, 2014-0322(2):355-64.
PMID: 24534551 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassayLei N et al. (2014-01-06), Intestinal subepithelial myofibroblasts support the growth of intestinal epithelial stem cells. PLoS ONE, 2014-09(1):e84651.
PMID: 24400106 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayWu C et al. (2014-01-01), RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun, 2014-05(0):3149.
PMID: 24476626 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayLee J et al. (2013-11-19), Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. Elife, 2013-12(0):e00940.
PMID: 24252877 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayLee et al. (2013-10-31), Neuregulin autocrine signaling promotes self-renewal of breast tumor-initiating cells by triggering HER2/HER3 activation. Cancer Res, 2013-174(1):341-52.
PMID: 24177178 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayWilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448 -
Species: Human
Sample Types: Whole Cells
Applications: Bioassay(2012-05-04), Role of Gadd45a in Wip1-dependent regulation of intestinal tumorigenesis. Cell Death Differ., 2012-019(11):1761-8.
PMID: 22555459 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayHao HX et al. (2012-04-29), ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature, 2012-0485(7397):195-200.
PMID: 22575959 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySpence JR et al. (2010-12-12), Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 2010-1470(7332):105-9.
PMID: 21151107
There are no citations that match your criteria.
Customer Reviews for Recombinant Human R-Spondin 1 Protein (6)
Customer Images
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Application: 3D cell culture/organoids.Verified Customer | Posted 10/11/2023
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Application: 3D culture of cellsVerified Customer | Posted 03/04/2023R-Spondin was used for colonocyte isolation and culturing from Sprague-Dawley rats in 3D culture. Results showed a purified culture as spheroids-like, following a standard isolation protocol for these cells
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 09/26/2021Used for the differentiation of iPSC into intestinal organoids, at a concentration of 500ng/mL
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Application: Cell CultureVerified Customer | Posted 01/02/2021
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 07/24/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 03/06/2020
There are no reviews that match your criteria.
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