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Human/Mouse Desmin Antibody
Catalog # AF3844 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Summary for Human/Mouse Desmin Antibody
Immunogen
E. coli-derived recombinant human Desmin
Val261-Leu470
Accession # P17661
Val261-Leu470
Accession # P17661
Specificity
Detects human and mouse Desmin.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Desmin Antibody
Desmin in Human Heart.
Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Desmin in Mouse Skeletal Muscle.
Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse Desmin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart and immersion fixed frozen sections of mouse skeletal muscle
Sample: Immersion fixed paraffin-embedded sections of human heart and immersion fixed frozen sections of mouse skeletal muscle
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using AF3844 in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmin
Product Documents for Human/Mouse Desmin Antibody
Product Specific Notices for Human/Mouse Desmin Antibody
For research use only
Citations for Human/Mouse Desmin Antibody (16)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Desmin Antibody include:
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Species: Human
Sample Types: Whole Tissue
Applications: IHCA Brodehl et al. (2021-10-05), The Desmin Mutation DES-c.735G>C Causes Severe Restrictive Cardiomyopathy by Inducing In-Frame Skipping of Exon-3 Biomedicines, 2021-19(10).
PMID: 34680517 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCLE Bruijn et al. (2020-11-16), Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape J Am Heart Assoc, 2020-19(23):e017094.
PMID: 33190596 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrM Hara-Chiku et al. (2020-11-09), Inhibition of aquaporin-3 in macrophages by a monoclonal antibody as potential therapy for liver injury Nat Commun, 2020-111(1):5666.
PMID: 33168815 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCX Han et al. (2019-02-14), Lineage Tracing Reveals the Bipotency of SOX9+ Hepatocytes during Liver Regeneration Stem Cell Reports, 2019-012(3):624-638.
PMID: 30773487 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCA Dubrac et al. (2018-08-27), NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy Nat Commun, 2018-09(1):3463.
PMID: 30150707 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PC Szot et al. (2018-06-04), Tumor stroma-targeted antibody-drug conjugate triggers localized anticancer drug release J. Clin. Invest., 2018-00(0).
PMID: 29863500 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCHS Lee et al. (2018-04-27), Altered AKAP12 expression in portal fibroblasts and liver sinusoids mediates transition from hepatic fibrogenesis to fibrosis resolution Exp. Mol. Med., 2018-050(4):48.
PMID: 29700280 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCCA Lyon et al. (2017-12-11), Aneurysm Severity is Increased by Combined Mmp-7 Deletion and N-cadherin Mimetic (EC4-Fc) Over-Expression Sci Rep, 2017-17(1):17342.
PMID: 29229950 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PJ Kloepper et al. (2016-04-04), Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival Proc Natl Acad Sci USA, 2016-0113(16):4476-81.
PMID: 27044098 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PTE Peterson et al. (2016-04-04), Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages Proc Natl Acad Sci USA, 2016-0113(16):4470-5.
PMID: 27044097 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrLiu Q et al. (2015-12-04), Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes. Cell Res, 2015-126(1):119-30.
PMID: 26634606 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PRiordan D et al. (2015-07-15), Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 2015-010(7):e0128975.
PMID: 26176839 -
Species: Human
Sample Types: Whole Cells
Applications: IHCMaury Y et al. (2014-11-10), Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol, 2014-133(1):89-96.
PMID: 25383599 -
Species: Human
Sample Types: Whole Cells
Applications: ICCDurruthy-Durruthy et al. (2014-04-09), Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. PLoS ONE, 2014-09(4):e94231.
PMID: 24718618 -
Species: Human
Sample Types: Whole Cells
Applications: IHC-FrBrodehl A et al. (2013-11-07), The novel desmin mutant p.A120D impairs filament formation, prevents intercalated disk localization, and causes sudden cardiac death. Circ Cardiovasc Genet, 2013-16(6):615-23.
PMID: 24200904 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ICC,Western BlotBrodehl A et al. (2012-03-08), Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants. J. Biol. Chem., 2012-0287(19):16047-57.
PMID: 22403400
There are no citations that match your criteria.
Customer Reviews for Human/Mouse Desmin Antibody (2)
4.5 out of 5
2 customer
ratings
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Application: Western BlotSample Tested: Heart tissueSpecies: CatVerified Customer | Posted 10/27/20181:1000 in 1%milk/TBST overnight at 4C; washed 10 min for 3 times and incubate with mouse-anti-goat-HRP monoclonal antibody for 1 hour; washed for another 5 min 3 times. Developed with ECL.
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Application: Immunohistochemistry-ParaffinSample Tested: See PMID 24200904Species: HumanVerified Customer | Posted 01/08/2015
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