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Human/Mouse/Rat Vimentin Antibody
Catalog # MAB2105 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 280618
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Product Summary for Human/Mouse/Rat Vimentin Antibody
Immunogen
E. coli-derived recombinant human Vimentin
Ser2-Glu466
Accession # P08670
Ser2-Glu466
Accession # P08670
Specificity
Vimentin antibodies are ideal for immunocytochemistry colocalization studies in intermediate filaments. Detects human, mouse and rat Vimentin in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat Vimentin Antibody
Detection of Human Vimentin by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat Vimentin by Western Blot.
Western blot shows lysates of MEF mouse embryonic feeder cells, NIH-3T3 mouse embryonic fibroblast cell line, Rat-2 rat embryonic fibroblast cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Vimentin in NTera‑2 Human Cell Line.
Vimentin was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Vimentin Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human neural progenitor cells, NTera-2 human testicular embryonic carcinoma cell line, A549 human lung carcinoma cell line, mouse cortical stem cells, and rat cortical stem cells
Sample: Immersion fixed human neural progenitor cells, NTera-2 human testicular embryonic carcinoma cell line, A549 human lung carcinoma cell line, mouse cortical stem cells, and rat cortical stem cells
Immunohistochemistry
0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: A172 human glioblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Knockout Validated
Vimentin
is specifically detected in K562 human chronic myelogenous leukemia parental cell line but is not detectable in
Vimentin knockout K562 cell line.
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Western Blot
1-2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MEF mouse embryonic feeder cells, NIH‑3T3 mouse embryonic fibroblast cell line, Rat‑2 rat embryonic fibroblast cell line, and NR8383 rat alveolar macrophage cell line
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MEF mouse embryonic feeder cells, NIH‑3T3 mouse embryonic fibroblast cell line, Rat‑2 rat embryonic fibroblast cell line, and NR8383 rat alveolar macrophage cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 6 reviews rated 4.5 using MAB2105 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vimentin
Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).
References
- Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
- Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
- Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
- Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
- Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
- Li, Q-F. et al. (2006) J. Biol. Chem. 281:34716.
- Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
- Styers, M.L. et al. (2005) Traffic 6:359.
- McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
- Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
- Ivaska, J. et al. (2005) EMBO J. 24:3834.
- Byun, Y. et al. (2001) Cell Death Differ. 8:443.
- Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
- Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
- Garg, A. et al. (2006) J. Immunol. 177:6192.
Alternate Names
VIM
Gene Symbol
VIM
UniProt
Product Documents for Human/Mouse/Rat Vimentin Antibody
Product Specific Notices for Human/Mouse/Rat Vimentin Antibody
For research use only
Citations for Human/Mouse/Rat Vimentin Antibody (21)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Vimentin Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: ICCPolisetti et al. (2023-04-25), Efficient Isolation and Expansion of Limbal Melanocytes for Tissue Engineering International journal of molecular sciences
PMID: 37175529 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCMD Lauver et al. (2022-11-07), T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus Elife, 2022-111(0).
PMID: 36341713 -
Species: Human
Sample Types: Whole Cells
Applications: ICCR Chimienti et al. (2022-09-27), Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-040(13):111423.
PMID: 36170817 -
Species: Mouse
Sample Types: Whole Cells
Applications: IFC Rodriguez- et al. (2022-06-15), NR2F1 is a barrier to dissemination of early stage breast cancer cells Cancer Research, 2022-00(0).
PMID: 35471456 -
Species: Human
Sample Types: Organoids
Applications: IHCMM Capeling et al. (2022-02-15), Suspension culture promotes serosal mesothelial development in human intestinal organoids Cell Reports, 2022-038(7):110379.
PMID: 35172130 -
Species: Human
Sample Types: Whole Cells
Applications: ICCde Rus Jacquet A et al. (2021-09-30), The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson's disease. Elife, 2021-010(0).
PMID: 34590578 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCM Cui et al. (2021-09-06), Nrf1 promotes heart regeneration and repair by regulating proteostasis and redox balance Nature Communications, 2021-012(1):5270.
PMID: 34489413 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCGT Park et al. (2021-04-23), Echinochrome A Treatment Alleviates Fibrosis and Inflammation in Bleomycin-Induced Scleroderma Marine Drugs, 2021-019(5).
PMID: 33922418 -
Species: Mouse
Sample Types: Whole Tissue,Whole Cells
Applications: IHC,ICCD Yamakawa et al. (2021-03-09), Primary cilia-dependent lipid raft/caveolin dynamics regulate adipogenesis Cell Reports, 2021-034(10):108817.
PMID: 33691104 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryLA Tashireva et al. (2021-03-02), Heterogeneous Manifestations of Epithelial-Mesenchymal Plasticity of Circulating Tumor Cells in Breast Cancer Patients International Journal of Molecular Sciences, 2021-022(5).
PMID: 33801519 -
Species: Human
Sample Types: Organoid
Applications: IHCSK Sarvestani et al. (2021-01-11), Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 2021-012(1):262.
PMID: 33431859 -
Species: Human
Sample Types: Whole Cells
Applications: ICCZ Wang et al. (2020-12-08), Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution Cell Reports, 2020-133(10):108472.
PMID: 33296652 -
Species: Rabbit
Sample Types: Whole Cells
Applications: ICCP Saikia et al. (2020-10-07), Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea Sci Rep, 2020-110(1):16717.
PMID: 33028893 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryA Lopresti et al. (2019-06-13), Sensitive and easy screening for circulating tumor cells by flow cytometry JCI Insight, 2019-05(0).
PMID: 31194699 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PS Wichaiyo et al. (2019-02-07), Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice Haematologica, 2019-00(0).
PMID: 30733265 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotCK Cajigas-Du et al. (2018-07-13), RNA sequencing reveals upregulation of a transcriptomic program associated with stemness in metastatic prostate cancer cells selected for taxane resistance Oncotarget, 2018-09(54):30363-30384.
PMID: 30100995 -
Species: Xenograft
Sample Types: Whole Cells
Applications: Flow CytometryM Verheecke et al. (2018-02-13), Acute Drug Effects on the Human Placental Tissue: The Development of a Placental Murine Xenograft Model Reprod Sci, 2018-00(0):1933719118756.
PMID: 29439620 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCProcopio M et al. (2015-08-24), Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation. Nat Cell Biol, 2015-017(9):1193-204.
PMID: 26302407 -
da Silva Antunes R et al. (2015-07-24), The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells. J Immunol, 2015-0195(5):2429-41.
PMID: 26209626 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCRiess J et al. (2015-02-01), GLI1, CTNNB1 and NOTCH1 protein expression in a thymic epithelial malignancy tissue microarray. 2015-00(0).
PMID: 25667444 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PVrana NE et al. (2008-08-15), Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures. Invest. Ophthalmol. Vis. Sci., 2008-049(12):5325-31.
PMID: 18708614
There are no citations that match your criteria.
Customer Reviews for Human/Mouse/Rat Vimentin Antibody (6)
4.5 out of 5
6 customer
ratings
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Epithelial cellsSpecies: MouseVerified Customer | Posted 06/19/2022
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Human fibroblastSpecies: HumanVerified Customer | Posted 06/15/2021Vimentin expression in human fibroblasts in culture. Vimentin (green) was used as a fibroblast marker in miofibroblast differentiation assays (a-SMA in red, as a miofibroblast marker).
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Mouse fibroblast cell lineSpecies: MouseVerified Customer | Posted 02/26/2021
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Application: ImmunohistochemistrySample Tested: Skin tissueSpecies: Rhesus MacaqueVerified Customer | Posted 06/23/2020
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Application: Western BlotSample Tested: Cancer cell lysatesSpecies: HumanVerified Customer | Posted 11/14/2019Testing several cell lines for mesenchymal characteristics, including vimentin expression, by western blot.
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Application: Immunohistochemistry-FrozenSample Tested: Cultured Human KeratinocytesSpecies: HumanVerified Customer | Posted 02/22/2019
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