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Rat F(ab)2 IgG (H+L) PE‑conjugated Antibody

Catalog # F0105B | R&D Systems, Inc. a Bio-Techne Brand
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F0105B

Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG

Product Summary for Rat F(ab)2 IgG (H+L) PE‑conjugated Antibody

Immunogen

Rat IgG heavy and light chains

Specificity

Detects rat IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate mouse cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat F(ab)2 IgG (H+L) PE‑conjugated Antibody

Detection of Rat IgG Primary Antibody by Flow Cytometry

Detection of Rat IgG Primary Antibody by Flow Cytometry

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse MMR/CD206 Monoclonal Antibody (Catalog # MAB25351, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) at 10 µL/106cells.

Applications for Rat F(ab)2 IgG (H+L) PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rat origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using F0105B in the following applications:

Published Applications

Read 4 publications using F0105B in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Product Documents for Rat F(ab)2 IgG (H+L) PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat F(ab)2 IgG (H+L) PE‑conjugated Antibody

For research use only

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