Mouse MMR/CD206 Antibody

Name: Mouse MMR/CD206 Antibody
Brand: R&D Systems
SKU: MAB25351

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB25351

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Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse MMR Leu19-Ala1388 Accession # Q61830
Applications:	CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Rat IgG₁ Clone # 857615
Specificity:	Detects mouse MMR in direct ELISAs.

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Applications for Mouse MMR/CD206 Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse MMR/CD206 Antibody

	Flow Cytometry Detection of MMR/CD206 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse MMR/CD206 Monoclonal Antibody (Catalog # MAB25351, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: MMR/CD206

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) mouse CD206 precursor contains a signal sequence (19 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The mouse CD206 ECD shares 96% aa sequence identity with rat MR, and 83‑84% with human, equine, porcine and canine CD206. The cysteine-rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewis^a and ‑Lewis^x (1, 7, 9). The FNII domain mediates Ca2⁺‑independent binding of collagens (2, 10). The CTLDs participate in Ca2⁺‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin-coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

Item	Value
References	Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177. Harris, N. et al. (1992) Blood 80:2363. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156. Chieppa, M. et al. (2003) J. Immunol. 171:4552. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. Taylor, P.R. et al. (2005) Trends Immunol. 26:104. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179. Leteux, C. et al. (2000) J. Exp. Med. 191:1117. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.
Long Name	Macrophage Mannose Receptor
Entrez Gene IDs	4360 (Human); 17533 (Mouse); 291327 (Rat)
Alternate Names	C-type lectin domain family 13 member D, CD206, CLEC13D, CLEC13Dmacrophage mannose receptor 1, MMR, MMRCD206 antigen, MRC1, mannose receptor, C type 1

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Mouse MMR/CD206 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Mouse MMR/CD206 Antibody

Scientific Data Examples for Mouse MMR/CD206 Antibody

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: MMR/CD206

Citations for Mouse MMR/CD206 Antibody

Product Documents for Mouse MMR/CD206 Antibody

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