J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse MMR/CD206 APC-conjugated Monoclonal Antibody (Catalog # FAB25351A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse MMR/CD206 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMR/CD206

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) mouse CD206 precursor contains a signal sequence (19 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The mouse CD206 ECD shares 96% aa sequence identity with rat MR, and 83‑84% with human, equine, porcine and canine CD206. The cysteine-rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewis^a and ‑Lewis^x (1, 7, 9). The FNII domain mediates Ca2⁺‑independent binding of collagens (2, 10). The CTLDs participate in Ca2⁺‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin-coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

References

Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
Harris, N. et al. (1992) Blood 80:2363.
Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
Chieppa, M. et al. (2003) J. Immunol. 171:4552.
Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Entrez Gene IDs

4360 (Human); 17533 (Mouse); 291327 (Rat)

Gene Symbol

MRC1

UniProt

Q61830

Additional MMR/CD206 Products

Product Documents for Mouse MMR/CD206 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse MMR/CD206 APC-conjugated Antibody

For research use only

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Mouse MMR/CD206 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB25351A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all MMR/CD206 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse MMR/CD206 APC-conjugated Antibody

Detection of MMR/CD206 in J774A.1 Mouse Cell Line by Flow Cytometry.

Applications for Mouse MMR/CD206 APC-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: MMR/CD206

References

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional MMR/CD206 Products

Product Documents for Mouse MMR/CD206 APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Mouse MMR/CD206 APC-conjugated Antibody