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Rat IgG Horseradish Peroxidase-conjugated Antibody Best Seller

Catalog # HAF005 | R&D Systems, Inc. a Bio-Techne Brand
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HAF005

Key Product Details

Species Reactivity

Rat

Applications

ELISA, Immunohistochemistry, Simple Western, Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG

Product Summary for Rat IgG Horseradish Peroxidase-conjugated Antibody

Immunogen

Rat IgG

Specificity

Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat IgG Horseradish Peroxidase-conjugated Antibody

Detection of Rat IgG Primary Antibody by Western Blot

Detection of Rat IgG Primary Antibody by Western Blot.

Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a Monoclonal Antibody (MAB645) using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat IgG Primary Antibody by Simple WesternTM.

Detection of Rat IgG Primary Antibody by Simple WesternTM.

Simple Western lane view shows detection of Rat Anti-Mouse S100A9 Monoclonal Antibody ( MAB2065) using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Rat IgG Primary Antibody by Immunohistochemistry.

Detection of Vimentin in human kidney using 5 ug/mL of Rat monoclonal anti-human Vimentin primary antibody (MAB2105) followed by 30 min incubation with Goat anti Rat HRP conjugated secondary antibodies (Catalog # HAF005) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.

Applications for Rat IgG Horseradish Peroxidase-conjugated Antibody

Application
Recommended Usage

ELISA

Use at a 1:1000 dilution.

Immunohistochemistry

Use at a 1:20 dilution 

Simple Western

Use at a 1:50 dilution.

Western Blot

Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 4.4 using HAF005 in the following applications:

Published Applications

Read 27 publications using HAF005 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG, IGHG1, Immunoglobulin G, ImmunoglobulinG

Product Documents for Rat IgG Horseradish Peroxidase-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat IgG Horseradish Peroxidase-conjugated Antibody

For research use only

Citations for Rat IgG Horseradish Peroxidase-conjugated Antibody (27)

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Customer Reviews for Rat IgG Horseradish Peroxidase-conjugated Antibody (5)

4.4 out of 5
5 customer ratings
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  • Rat IgG HRP-conjugated Antibody
    Name: Amruta Narayanappa
    Application: Western Blot
    Sample Tested: bEnd.3 mouse endothelial cell
    Species: Mouse
    Verified Customer | Posted 11/27/2020
    It works great for western blot
  • Rat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mv1Lu mink lung epithelial cell line
    Species: Mink
    Verified Customer | Posted 11/20/2019
    Rat IgG Horseradish Peroxidase-conjugated Antibody HAF005
  • Rat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lung single-cell suspension
    Species: Mouse
    Verified Customer | Posted 06/06/2019
    Rat IgG Horseradish Peroxidase-conjugated Antibody HAF005
  • Rat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Mouse
    Verified Customer | Posted 05/20/2019
    Primary antibody: rat anti-mouse CD45. Sample: 40 ug and 100 ug cell lysis.
    Rat IgG Horseradish Peroxidase-conjugated Antibody HAF005
  • Rat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
    Species: Mouse
    Verified Customer | Posted 09/30/2018
    Rat IgG Horseradish Peroxidase-conjugated Antibody HAF005

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