Human/Mouse Wnt-5a Antibody

Name: Human/Mouse Wnt-5a Antibody
Brand: R&D Systems
SKU: MAB645
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB645

Citations (17)

(1)

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Species Reactivity:	Human, Mouse
Immunogen:	CHO-derived recombinant mouse Wnt-5a Gln38-Lys380 Accession # P22725
Applications:	Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 442625
Specificity:	Detects mouse Wnt-5a in direct ELISAs and human and mouse Wnt‑5a in Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, 2b, 3a, 4, 5b, 8a, 8b, 9b, 10a, 10b, 11, or 16 is observed.

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Applications for Human/Mouse Wnt-5a Antibody

	Recommended Concentration	Sample
Western Blot	HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c.) tissue
Immunohistochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse Wnt-5a Antibody

	Western Blot Detection of Human and Mouse Wnt‑5a by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-5a at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry Wnt‑5a in Mouse Embryo. Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Wnt-5a

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23‑24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca²⁺ pathway, and the planar cell polarity (1‑2).

Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mature Wnt-5a is a 49 kDa protein that shares 99% amino acid identity in mouse, rat and human.

Item	Value
References	Miller, J.R. (2002) Genome Biol. 3:3001. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381. Veeman, M.T. et al. (2003) Developmental Cell 5:367. Topol, L. et al. (2003) J. Cell Biol 162:899. Torres, M. et al. (1996) J. Cell Biol. 133:1123. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
Long Name	Wingless-type MMTV Integration Site Family, Member 5a
Entrez Gene IDs	7474 (Human); 22418 (Mouse)
Alternate Names	WNT-5A protein, Wnt-5a, Wnt5a, hWNT5A, protein Wnt-5a, wingless-type MMTV integration site family, member 5A

Citations for Human/Mouse Wnt-5a Antibody (17)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Wnt-5a Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

GJ Lee et al. (2022), YAP-dependent Wnt5a induction in hypertrophic adipocytes restrains adiposity Cell Death Disease, 2022;13(4):407.
PMID: 35478181
Species: Human
Sample Types: Protein Extracts
Applications: Western Blot

V Yadav et al. (2022), Increased MARCKS Activity in BRAF Inhibitor-Resistant Melanoma Cells Is Essential for Their Enhanced Metastatic Behavior Independent of Elevated WNT5A and IL-6 Signaling Cancers, 14(24).
PMID: 36551563
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Y Shao et al. (2021), HDAC6 suppresses microRNA-199a transcription and augments HPV-positive cervical cancer progression through Wnt5a upregulation The international journal of biochemistry cell biology, 2021;0(0):106000.
PMID: 33933678
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

T Radaszkiew et al. (2021), RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy Elife, 10(0).
PMID: 34702444
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Asem et al. (2020), Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis Cancer Res., 0(0).
PMID: 31932454
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

TC Huang et al. (2017), Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells PLoS ONE, 12(8):e0181034.
PMID: 28859077
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

M Vesel et al. (2017), ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling Respir. Res, 18(1):52.
PMID: 28340578
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Hoeke A Baarsma (2016), Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD J. Exp. Med, 0(0).
PMID: 27979969
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

(2016), WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition J Exp Clin Cancer Res, 35(1):144.
PMID: 27623766
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Fuster J et al. (2015), Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion. Diabetes, 64(4):1235-48.
PMID: 25352637
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

MacMillan et al. (2014), Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway. Sci Rep, 4(0):6315.
PMID: 25204426
Species: Human
Sample Types: Protein
Applications: Western Blot

Yap L et al. (2014), Oncogenic effects of WNT5A in Epstein-Barr virusassociated nasopharyngeal carcinoma. Int J Oncol, 44(5):1774-80.
PMID: 24626628
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Linnskog R et al. (2014), Interleukin-6 drives melanoma cell motility through p38alpha-MAPK-dependent up-regulation of WNT5A expression. Mol Oncol, 8(8):1365-78.
PMID: 24954857
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Zhao Y et al. (2014), Wnt5a promotes inflammatory responses via nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells. J Biol Chem, 289(30):21028-39.
PMID: 24891513
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Popp T et al. (2014), Wnt5a/beta-catenin signaling drives calcium-induced differentiation of human primary keratinocytes. J Invest Dermatol, 134(8):2183-91.
PMID: 24658506
Species: Human
Sample Types: Ascites Fluid
Applications: Western Blot

Barbolina MV et al. (2011), Diverse mechanisms for activation of Wnt signalling in the ovarian tumour microenvironment. Biochem. J., 437(1):1-12.
PMID: 21668411
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Hasan M et al. (0), Wnt5a causes ROR1 to complex and activate cortactin to enhance migration of chronic lymphocytic leukemia cells. Leukemi33(3):653-661.
PMID: 30568170

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Human/Mouse Wnt-5a Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: E11.5 mouse embryo fixed in 4% PFA

Species: Mouse

Verified Customer | Posted 12/09/2020

Staining was done on E11.5 mouse embryo heart sections (Right Ventricle) (4% PFA fixed).Concentration used - 8ug/mL.

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Human/Mouse Wnt-5a Antibody

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Applications for Human/Mouse Wnt-5a Antibody

Scientific Data Examples for Human/Mouse Wnt-5a Antibody

Western Blot

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Wnt-5a

Citations for Human/Mouse Wnt-5a Antibody (17)

Customer Reviews for Human/Mouse Wnt-5a Antibody (1)

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Human/Mouse Wnt-5a Antibody

Product Documents for Human/Mouse Wnt-5a Antibody

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