Human Chromogranin A Antibody

Name: Human Chromogranin A Antibody
Brand: R&D Systems
SKU: MAB90981
Rating: 5 (1 reviews)

Catalog # MAB90981

Citations (1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Human embryonic kidney cell line HEK293-derived human Chromogranin A Met1-Gly457 Accession # P10645
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 948128
Specificity:	Detects human Chromogranin A in direct ELISAs.

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Applications

	Recommended Concentration	Sample
Immunohistochemistry	5-25 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human Chromogranin A Antibody Scientific Data Examples

	Immunocytochemistry Chromogranin A in H69AR Human Cell Line. Chromogranin A was detected in immersion fixed H69AR human small cell lung cancer cell line using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry Chromogranin A in Human Parathyroid. Chromogranin A was detected in immersion fixed paraffin-embedded sections of human parathyroid using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to principal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chromogranin A

Human Chromogranin A (CHGA) an acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A expression has been oberserved in neuroendocrine tumors, medullary thyroid carcinoma, as well as carcinoid tumors.

Item	Value
Entrez Gene IDs	1113 (Human); 12652 (Mouse); 24258 (Rat)
Alternate Names	Betagranin, CGA, CHGA, Catestatin, Chromofungin, Chromogranin A, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, betagranin (N-terminal fragment of chromogranin A), chromogranin A (parathyroid secretory protein 1), chromogranin-A

Citations for Human Chromogranin A Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human Chromogranin A Antibody include:

Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

H Chen et al. (2020), Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production JCI Insight, 5(17).
PMID: 32879135

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Human Chromogranin A Antibody

Name: Anonymous

Application: IHC

Sample Tested: Prostatic adenocarcinoma tissue,Tumor tissue

Species: Human

Verified Customer | Posted 12/07/2021

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Human Chromogranin A Antibody

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Key Product Details

Complete Your Research

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Applications

Human Chromogranin A Antibody Scientific Data Examples

Immunocytochemistry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Chromogranin A

Citations for Human Chromogranin A Antibody (1)

Customer Reviews (1)

Customer Images

Human Chromogranin A Antibody

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

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