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Human Chromogranin A Antibody

Catalog # MAB90981 | R&D Systems, Inc. a Bio-Techne Brand
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MAB90981
MAB90981-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 948128

Product Summary for Human Chromogranin A Antibody

Immunogen

Human embryonic kidney cell line HEK293-derived human Chromogranin A 
Met1-Gly457
Accession # P10645

Specificity

Detects human Chromogranin A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Chromogranin A Antibody

Chromogranin A antibody in H69AR Human Cell Line by Immunocytochemistry (ICC).

Chromogranin A in H69AR Human Cell Line.

Chromogranin A was detected in immersion fixed H69AR human small cell lung cancer cell line using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Chromogranin A antibody in Human Parathyroid by Immunohistochemistry (IHC-P).

Chromogranin A in Human Parathyroid.

Chromogranin A was detected in immersion fixed paraffin-embedded sections of human parathyroid using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to principal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Chromogranin A Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed H69AR human small cell lung cancer cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human parathyroid
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB90981 in the following applications:

Published Applications

Read 1 publication using MAB90981 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chromogranin A

Human Chromogranin A (CHGA) an acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A expression has been oberserved in neuroendocrine tumors, medullary thyroid carcinoma, as well as carcinoid tumors.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Entrez Gene IDs

1113 (Human); 12652 (Mouse); 24258 (Rat)

Gene Symbol

CHGA

UniProt

Product Documents for Human Chromogranin A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Chromogranin A Antibody

For research use only

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FAQs for Human Chromogranin A Antibody

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  • Q: I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?

    A: When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

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Reconstitution Calculator

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