Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) at a different concentration than recommended?

We cannot guarantee the performance if the protein is reconstituted at a different concentration than recommended in the product datasheet. Please contact Technical Service if you have any questions or concerns about reconstitution.

Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) in a different buffer than recommended?

For best recovery, stability, and activity, the protein should be reconstituted in the buffer recommended on the product datasheet. Stability testing has been evaluated under these conditions; we can support the performance under these reconstitution conditions.

Recombinant Human Wnt-3a Protein

Name: Recombinant Human Wnt-3a Protein
Brand: R&D Systems
SKU: 5036-WN
Rating: 5 (25 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 5036-WN

Citations (319)

(25)

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Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	NP_149122
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Wnt-3a Protein

Product Specifications for Recombinant Human Wnt-3a Protein

Source	Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein Ser19-Lys352
Purity	>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser19
Predicted Molecular Mass	37.4 kDa
SDS-PAGE	40 kDa, reducing conditions
Activity	Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 5.00-25.0 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.

Scientific Data Examples for Recombinant Human Wnt-3a Protein

	Recombinant Human Wnt-3a Protein Bioactivity Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. The ED₅₀ is 1.7-fold better with more than twice the maximum response compared to the top competitor's Wnt-3a.
	Recombinant Human Wnt-3a Protein Lot to Lot Consistency The lot-to-lot consistency of Recombinant Human Wnt-3a Protein (Catalog # 5036-WN) was assessed by testing the ability of three independent lots of the protein to induce alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. Each trace on the graph represents data obtained from Recombinant Human Wnt-3a from a different manufacturing run. The ED₅₀ for this effect is 5.00-25.0 ng/mL.
	iPSC-derived Human Intestinal Organoids Cultured using Recombinant Human Wnt-3a Protein. iPSC-derived human intestinal organoids were cultured using Cultrex™ UltiMatrix RGF Basement Membrane Extract (BME001-05) and intestinal organoid culture medium, which includes Recombinant Human Wnt-3a (Catalog # 5036-WN), Recombinant Human EGF (236-EG), Recombinant Human Noggin (6057-NG), and Recombinant Human R-Spondin 1 (4645-RS), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; green) and a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (5748; blue).
	Adult Stem Cell-derived Human Descending Colon Organoids Cultured using Recombinant Human Wnt-3a Protein. Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and cultured for 30 days in intestinal organoid culture medium, which includes Recombinant Human Wnt-3a (Catalog # 5036-WN), Recombinant Human EGF (236-EG), Recombinant Human Noggin (6057-NG), and Recombinant Human R-Spondin 1 (4645-RS), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; red) and DAPI (5748; blue). The image shown was taken at 10x magnification. (B) Organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; red) and DAPI (5748; blue). The image shown was taken at 20x magnification.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

5036-WN

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5036-WN/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-3a

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).

Item	Value
References	Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880. Korinek, V. et al. (1997) Science 275:1784. Chen, B. et al. (2009) Nat. Chem. Biol. 5:100. Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267. Takada, R. et al. (2006) Dev. Cell 11:791. Komekado, H. (2007) Genes Cells 12:521. Dunty Jr. W. C. et al. (2008) Development 135:85. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27. Lee, S. M. et al. (2000) Development 127:457. Takada, S. et al. (1994) Genes Dev. 8:174. Willert, K. et al. (2003) Nature 423:6938. Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970. Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.
Long Name	Wingless-type MMTV Integration Site Family, Member 3a
Entrez Gene IDs	89780 (Human); 22416 (Mouse)
Alternate Names	MGC119418, MGC119419, MGC119420, Wnt-3a, Wnt3a, protein Wnt-3a, wingless-type MMTV integration site family, member 3A

Citations for Recombinant Human Wnt-3a Protein (319)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Wnt-3a Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Stoletov et al. (2023-01-01), Intravital imaging of Wnt/beta-catenin and ATF2-dependent signalling pathways during tumour cell invasion and metastasis Journal of Cell Science, 0(0).
PMID: 36621522
Species: Frog
Sample Types: Embryo
Applications: Bioassay

C Seidl et al. (2023), Mucociliary Wnt signaling promotes cilia biogenesis and beating Nature Communications, 14(1):1259.
PMID: 36878953
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Geleta et al. (2022-01-01), Targeting Wnt/Tenascin C-Mediated Cross Talk between Pancreatic Cancer Cells and Stellate Cells via activation of the Metastasis Suppressor NDRG1 The Journal of Biological Chemistry, 0(0):101608.
PMID: 35065073
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Han et al. (2022-01-01), Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1 Cell Reports, 38(2):110240.
PMID: 35021086
Species: Human
Sample Types: Organoids
Applications: Bioassay

MM Capeling et al. (2022-01-01), Suspension culture promotes serosal mesothelial development in human intestinal organoids Cell Reports, 38(7):110379.
PMID: 35172130
Species: Human
Sample Types: Transduced Whole Cells
Applications: Bioassay

J Jeong et al. (2022-01-01), Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids Biology, 11(4).
PMID: 35453693
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Li et al. (2022-01-01), Hotspot ESR1 mutations are multimodal and contextual modulators of breast cancer metastasis Cancer Research, 0(0).
PMID: 35078818
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CJ Brereton et al. (2022-01-01), Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis Elife, 11(0).
PMID: 35188460
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CW Wasson et al. (2022-01-01), Induction of Pro-Fibrotic CLIC4 in Dermal Fibroblasts by TGF-beta/Wnt3a Is Mediated by GLI2 Upregulation Cells, 11(3).
PMID: 35159339
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Fu et al. (2022-01-01), Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9 Stem Cell Research, 62(0):102800.
PMID: 35561459
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

M Naumann et al. (2022-01-01), Combined Systemic Drug Treatment with Proton Therapy: Investigations on Patient-Derived Organoids Cancers, 14(15).
PMID: 35954444
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Condello et al. (2022-01-01), Tissue transglutaminase activates integrin-linked kinase and beta-catenin in ovarian cancer The Journal of Biological Chemistry, 0(0):102242.
PMID: 35810788
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Shen et al. (2022-01-01), The Casein kinase 1alpha agonist pyrvinium attenuates Wnt-mediated CK1alpha degradation via interaction with the E3 ubiquitin ligase component Cereblon The Journal of Biological Chemistry, 0(0):102227.
PMID: 35780831
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Tian et al. (2022-01-01), WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome Nature Communications, 13(1):5456.
PMID: 36114168
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

R Moser et al. (2022-01-01), Pharmacological targeting of TFIIH suppresses KRAS mutant pancreatic ductal adenocarcinoma and synergizes with TRAIL Cancer Research, 0(0).
PMID: 35819261
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Gupta et al. (2022-01-01), Inï¿½vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion Oncogene, 40(3):111119.
PMID: 35858555
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Shi et al. (2022-01-01), Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem Cells Stem Cells International, 2022(0):3222427.
PMID: 36467281
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Lungova et al. (2022-01-01), Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling Oncogene, 0(0).
PMID: 35770504
Species: Human
Sample Types: Whole Cell
Applications: Cell Culture

MG Lee et al. (2022-01-01), Nuclear S6K1 Enhances Oncogenic Wnt Signaling by Inducing Wnt/beta-Catenin Transcriptional Complex Formation International Journal of Molecular Sciences, 23(24).
PMID: 36555784
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

WC Hwang et al. (2022-01-01), Inhibition of phospholipase D1 induces immunogenic cell death and potentiates cancer immunotherapy in colorectal cancer Experimental Molecular Medicine, 2022;0(0).
PMID: 36131027
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

D Song et al. (2022-01-01), Insulin-like growth factor 2 mRNA-binding protein 3 promotes kidney injury by regulating beta-catenin signaling JCI Insight, 0(0).
PMID: 36520532
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Fregnani et al. (2022-01-01), CK1alpha/RUNX2 Axis in the Bone Marrow Microenvironment: A Novel Therapeutic Target in Multiple Myeloma Cancers, 14(17).
PMID: 36077711
Species: Human
Sample Types: Whole Cells
Applications: Stimulation

H Ngai et al. (2022-01-01), LEF1 drives a central memory program and supports antitumor activity of natural killer T cells Cancer Immunology Research, 0(0).
PMID: 36484736
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Nagano et al. (2022-01-01), R-spondin 3 deletion induces Erk phosphorylation to enhance Wnt signaling and promote bone formation in the appendicular skeleton Elife, 11(0).
PMID: 36321691
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Jiang et al. (2022-01-01), Ivermectin inhibits tumor metastasis by regulating the Wnt/beta-catenin/integrin beta1/FAK signaling pathway American journal of cancer research, 12(10):4502-4519.
PMID: 36381328
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

P Paclíková et al. (2021-01-01), Roles of individual human Dishevelled paralogs in the Wnt signalling pathways Cellular Signalling, 85(0):110058.
PMID: 34082011
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Baek et al. (2021-01-01), Ubiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells Cell Death Disease, 2021;12(3):238.
PMID: 33664230
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Akbari et al. (2021-01-01), LGR5/R-Spo1/Wnt3a axis promotes stemness and aggressive phenotype in hepatoblast-like hepatocellular carcinoma cell lines Cellular Signalling, 0(0):109972.
PMID: 33684507
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Quandt et al. (2021-01-01), Wnt-&beta-catenin activation epigenetically reprograms Treg cells in inflammatory bowel disease and dysplastic progression Nature Immunology, 0(0).
PMID: 33664518
Species: Human
Sample Types: Plasmid
Applications: Bioassay

J Du et al. (2021-01-01), ATF4 promotes lung cancer cell proliferation and invasion partially through regulating Wnt/&beta-catenin signaling International Journal of Medical Sciences, 18(6):1442-1448.
PMID: 33628101
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

I Fischer et al. (2021-01-01), Generation of human induced pluripotent stem cell lines from 2 patients with MIRAGE syndrome Stem Cell Research, 54(0):102417.
PMID: 34119956
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

PL Ramanujam et al. (2021-01-01), Global chromatin organizer SATB1 acts as a context-dependent regulator of the Wnt/Wg target genes Scientific Reports, 11(1):3385.
PMID: 33564000
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

YH Cui et al. (2021-01-01), Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis Nature Communications, 12(1):2183.
PMID: 33846348
Species: Human
Sample Types: Organoid
Applications: Bioassay

N Raj et al. (2021-01-01), Cell-type-specific profiling of human cellular models of fragile X syndrome reveal PI3K-dependent defects in translation and neurogenesis Cell Reports, 35(2):108991.
PMID: 33852833
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JN Kuehner et al. (2021-01-01), 5-hydroxymethylcytosine is dynamically regulated during forebrain organoid development and aberrantly altered in Alzheimer's disease Cell Reports, 35(4):109042.
PMID: 33910000
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

QD Deng et al. (2021-01-01), Triptolide suppresses the growth and metastasis of non-small cell lung cancer by inhibiting &beta-catenin-mediated epithelial-mesenchymal transition Acta pharmacologica Sinica, 0(0).
PMID: 33893396
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Sinha et al. (2021-01-01), Repression of Wnt/&beta-catenin signaling by SOX9 and Mastermind-like transcriptional coactivator 2 Science Advances, 7(8).
PMID: 33597243
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

O Kwon et al. (2021-01-01), The development of a functional human small intestinal epithelium model for drug absorption Science Advances, 7(23).
PMID: 34078609
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

TW Fowler et al. (2021-01-01), Development of selective bispecific Wnt mimetics for bone loss and repair Nature Communications, 12(1):3247.
PMID: 34059688
Species: Equine
Sample Types: Organoid
Applications: Bioassay

S Hellman (2021-01-01), Generation of equine enteroids and enteroid-derived 2D monolayers that are responsive to microbial mimics Veterinary research, 52(1):108.
PMID: 34391473
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AV Parent et al. (2021-01-01), Selective deletion of human leukocyte antigens protects stem cell-derived islets from immune rejection Cell Reports, 36(7):109538.
PMID: 34407395
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Yang et al. (2021-01-01), WNT/&beta-catenin-suppressed FTO expression increases m6A of c-Myc mRNA to promote tumor cell glycolysis and tumorigenesis Cell Death Disease, 2021;12(5):462.
PMID: 33966037
W Liu et al. (2021-01-01), KAT6A, a novel regulator of &beta-catenin, promotes tumorigenicity and chemoresistance in ovarian cancer by acetylating COP1 Theranostics, 11(13):6278-6292.
PMID: 33995658
Species: Human
Sample Types: Organoid
Applications: Bioassay

Y Kang et al. (2021-01-01), A human forebrain organoid model of fragile X syndrome exhibits altered neurogenesis and highlights new treatment strategies Nature Neuroscience, 0(0).
PMID: 34413513
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Radaszkiew et al. (2021-01-01), RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy Elife, 10(0).
PMID: 34702444
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KM Mackinlay et al. (2021-01-01), An in vitro stem cell model of human epiblast and yolk sac interaction Elife, 10(0).
PMID: 34403333
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Johnson Ch et al. (2021-01-01), Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs International Journal of Molecular Sciences, 22(19).
PMID: 34639189
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay

M Yang et al. (2021-01-01), KCTD11 inhibits progression of lung cancer by binding to beta-catenin to regulate the activity of the Wnt and Hippo pathways Journal of Cellular and Molecular Medicine, 0(0).
PMID: 34453479
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Shen et al. (2021-01-01), The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling Nature Communications, 12(1):5263.
PMID: 34489457
Species: Human
Sample Types: Organoids
Applications: Bioassay

SA Saenz et al. (2021-01-01), Small molecule allosteric inhibitors of RORgammat block Th17-dependent inflammation and associated gene expression in vivo PLoS ONE, 16(11):e0248034.
PMID: 34752458
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AK Eicher et al. (2021-01-01), Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells Cell Stem Cell, 0(0).
PMID: 34856121
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Hu et al. (2021-01-01), Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-beta-catenin-STAT3 signaling Cell Death and Differentiation, 0(0).
PMID: 34743203
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Kowalski-J et al. (2021-01-01), Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain Science Advances, 7(46):eabj7917.
PMID: 34757789
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Chesnokova et al. (2021-01-01), Local non-pituitary growth hormone is induced with aging and facilitates epithelial damage Cell Reports, 37(11):110068.
PMID: 34910915
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Q Yan et al. (2021-01-01), PGC7 promotes tumor oncogenic dedifferentiation through remodeling DNA methylation pattern for key developmental transcription factors Cell Death and Differentiation, 0(0).
PMID: 33500560
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Völkner et al. (2021-01-01), Patient-Specific iPSC-Derived Neural Differentiated and Hepatocyte-like Cells, Carrying the Compound Heterozygous Mutation p.V1023Sfs*15/p.G992R, Present the "Variant" Biochemical Phenotype of Niemann-Pick Type C1 Disease International Journal of Molecular Sciences, 22(22).
PMID: 34830064
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

R Santos et al. (2021-01-01), Deficient LEF1 expression is associated with lithium resistance and hyperexcitability in neurons derived from bipolar disorder patients Molecular Psychiatry, 0(0).
PMID: 33398088
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CL Wu et al. (2021-01-01), Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis Nature Communications, 12(1):362.
PMID: 33441552
Species: Human
Sample Types: Organoid
Applications: Bioassay

M Tajan et al. (2021-01-01), Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy Nature Communications, 12(1):366.
PMID: 33446657
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Gil-Recio et al. (2021-01-01), Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells Stem Cells International, 2021(0):5212852.
PMID: 34795766
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Romano et al. (2021-01-01), Pluripotent Stem Cell-Derived Hepatocytes Inhibit T Cell Proliferation In Vitro through Tryptophan Starvation Cells, 11(1).
PMID: 35011586
Species: Human
Sample Types: Organoid, Organoids
Applications: Bioassay

A Sagara et al. (2021-01-01), Repositioning of duloxetine to target pancreatic stellate cells Oncology Letters, 22(4):744.
PMID: 34466156
Species: Human
Sample Types: Whole Cell
Applications: Bioassay

L Chen et al. (2021-01-01), USP28 facilitates pancreatic cancer progression through activation of Wnt/beta-catenin pathway via stabilising FOXM1 Cell Death Disease, 2021;12(10):887.
PMID: 34584067
Species: Human
Sample Types: Organoid
Applications: Bioassay

K Husain et al. (2021-01-01), Farnesyl dimethyl chromanol targets colon cancer stem cells and prevents colorectal cancer metastasis Scientific Reports, 11(1):2185.
PMID: 33500430
Species: Human
Sample Types: Organoid
Applications: Bioassay

T Ebisudani et al. (2021-01-01), Direct derivation of human alveolospheres for SARS-CoV-2 infection modeling and drug screening Cell Reports, 0(0):109218.
PMID: 34038715
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

P Kozielewic et al. (2020-01-01), Structural insight into small molecule action on Frizzleds Nat Commun, 11(1):414.
PMID: 31964872
Species: Human

Applications: Cell Culture

FH Brasil da et al. (2020-01-01), SULF1 suppresses Wnt3A-driven growth of bone metastatic prostate cancer in perlecan-modified 3D cancer-stroma-macrophage triculture models PLoS ONE, 15(5):e0230354.
PMID: 32413029
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

A Fleischer et al. (2020-01-01), iPSC-Derived Intestinal Organoids from Cystic Fibrosis Patients Acquire CFTR Activity upon TALEN-Mediated Repair of the p.F508del Mutation Mol Ther Methods Clin Dev, 17(0):858-870.
PMID: 32373648
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Bowers et al. (2020-01-01), FASN-Dependent Lipid Metabolism Links Neurogenic Stem/Progenitor Cell Activity to Learning and Memory Deficits Cell Stem Cell, 0(0).
PMID: 32386572
Species: Human
Sample Types: Whole Cell
Applications: Bioassay

E Flores-Her et al. (2020-01-01), Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells Cell. Signal., 72(0):109636.
PMID: 32283254
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Yoshida et al. (2020-01-01), Generation of intestinal organoids derived from human pluripotent stem cells for drug testing Sci Rep, 10(1):5989.
PMID: 32249832
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F He et al. (2020-01-01), LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells Stem Cell Res Ther, 11(1):163.
PMID: 32345350
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Q Li et al. (2020-01-01), Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription Cell Stem Cell, 26(5):675-692.e8.
PMID: 32259481
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Shi et al. (2020-01-01), Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein Biosci. Rep., 40(7).
PMID: 32608477
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Y Wu et al. (2020-01-01), Expression of MALAT1 Promotes Trastuzumab Resistance in HER2 Overexpressing Breast Cancers Cancers (Basel), 12(7).
PMID: 32708561
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

W Kawarazaki et al. (2020-01-01), Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency J. Clin. Invest., 0(0).
PMID: 32597829
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

R Castro-Viñ et al. (2020-01-01), Generation and characterization of human induced pluripotent stem cells (iPSCs) from hand osteoarthritis patient-derived fibroblasts Sci Rep, 10(1):4272.
PMID: 32144293
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

R Griffiths et al. (2020-01-01), The Transcription Factor-microRNA Regulatory Network during hESC-chondrogenesis Sci Rep, 10(1):4744.
PMID: 32179818
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Komura et al. (2020-01-01), Induced pluripotent stem cell-derived tenocyte-like cells promote the regeneration of injured tendons in mice Sci Rep, 10(1):3992.
PMID: 32132649
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

WY Liao et al. (2020-01-01), Dishevelled 1-Regulated Superpotent Cancer Stem Cells Mediate Wnt Heterogeneity and Tumor Progression in Hepatocellular Carcinoma Stem Cell Reports, 14(3):462-477.
PMID: 32160521
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

V Uribe-Etxe et al. (2020-01-01), Wnt-3a Induces Epigenetic Remodeling in Human Dental Pulp Stem Cells Cells, 9(3).
PMID: 32156036
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

M Liu et al. (2020-01-01), A hepatocyte differentiation model reveals two subtypes of liver cancer with different oncofetal properties and therapeutic targets Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 32123069
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

A El-Sehemy et al. (2020-01-01), Norrin mediates tumor-promoting and -suppressive effects in glioblastoma via Notch and WNT J. Clin. Invest., 0(0).
PMID: 32182224
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

L Omer et al. (2020-01-01), Familial hypercholesterolemia class II low density lipoprotein-receptor response to statin treatment Dis Model Mech, 0(0).
PMID: 32005714
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

M Díaz-Corán et al. (2020-01-01), Norrin restores blood-retinal barrier properties after vascular endothelial growth factor-induced permeability J. Biol. Chem., 0(0).
PMID: 32086377
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

JC Lim et al. (2020-01-01), Activation of &beta-catenin by TGF-&beta1 promotes ligament-fibroblastic differentiation and inhibits cementoblastic differentiation of human periodontal ligament cells Stem Cells, 0(0).
PMID: 32930424
Species: Human
Sample Types: Organoid
Applications: Cell Culture

K Dunbar et al. (2020-01-01), Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1 Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 32971322
Species: Chlorocebus aethiops (green monkey)
Sample Types: Whole Cells
Applications: Cell Culture

J Wang et al. (2020-01-01), Wnt/&beta-catenin signaling pathway inhibits porcine reproductive and respiratory syndrome virus replication by enhancing the nuclear factor-&kappaB-dependent innate immune response Veterinary Microbiology, 251(0):108904.
PMID: 33181435
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

M Zhu et al. (2020-01-01), Fallopian Tube Basal Stem Cells Reproducing the Epithelial Sheets In Vitro-Stem Cell of Fallopian Epithelium Biomolecules, 10(9).
PMID: 32899226
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

S Wu et al. (2020-01-01), Generation of a human iPSC line CIBi008-A from amniotic fluid-derived cells of a fetus with &beta-thalassemia carrying variants of -28Aï¿½>ï¿½G and IVS-II-654Cï¿½>ï¿½T in HBB Stem Cell Res, 49(0):102074.
PMID: 33157391
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MP Srikanth et al. (2020-01-01), Elevated Dkk1 Mediates Downregulation of the Canonical Wnt Pathway and Lysosomal Loss in an iPSC Model of Neuronopathic Gaucher Disease Biomolecules, 10(12).
PMID: 33287247
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Wei et al. (2020-01-01), PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin Onco Targets Ther, 13(0):10025-10037.
PMID: 33116587
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

S Nagel et al. (2020-01-01), Aberrant expression of NKL homeobox genes HMX2 and HMX3 interferes with cell differentiation in acute myeloid leukemia PLoS ONE, 15(10):e0240120.
PMID: 33048949
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

W Chong et al. (2020-01-01), Aquaporin 1 promotes sensitivity of anthracycline chemotherapy in breast cancer by inhibiting &beta-catenin degradation to enhance TopoII&alpha activity Cell Death Differ., 0(0).
PMID: 32814878
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

AJ Bilotta et al. (2020-01-01), Propionate Enhances Cell Speed and Persistence to Promote Intestinal Epithelial Turnover and Repair Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 33238220
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Pagliari et al. (2020-01-01), YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification Cell Death Differ, 0(0).
PMID: 33116297
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Michielin et al. (2020-01-01), The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs Cell Rep, 33(9):108453.
PMID: 33264615
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

PN Kluz et al. (2020-01-01), Cancer cell-intrinsic function of CD177 in attenuating beta-catenin signaling Oncogene, 39(14):2877-2889.
PMID: 32042113
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Moiseenko et al. (2020-01-01), Identification of a Repair-Supportive Mesenchymal Cell Population during Airway Epithelial Regeneration Cell Reports, 33(12):108549.
PMID: 33357434
Species: Human
Sample Types: Whole Cell
Applications: Cell Culture

JP Gleeson et al. (2020-01-01), Development of Physiologically Responsive Human iPSC-Derived Intestinal Epithelium to Study Barrier Dysfunction in IBD Int J Mol Sci, 21(4).
PMID: 32093254
Species: Human
Sample Types: Organoid, Whole Cells
Applications: Bioassay

L Zhang et al. (2020-01-01), Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 0(0).
PMID: 32783886
Species: Human
Sample Types: Cell Lysates, Organoid
Applications: Bioassay, Cell Culture

Z Dantes et al. (2020-01-01), Implementing cell-free DNA of pancreatic cancer patient-derived organoids for personalized oncology JCI Insight, 5(15).
PMID: 32614802
Species: Human
Sample Types: Whole Cells
Applications: Bioassay, Cell Culture

Y Gong et al. (2020-01-01), R-Spondin 2 Induces Odontogenic Differentiation of Dental Pulp Stem/Progenitor Cells via Regulation of Wnt/beta-Catenin Signaling Front Physiol, 11(0):918.
PMID: 32848860
Species: Human
Sample Types: Organoid
Applications: Bioassay

T Sawada et al. (2020-01-01), Developmental excitation-inhibition imbalance underlying psychoses revealed by single-cell analyses of discordant twins-derived cerebral organoids Mol. Psychiatry, 0(0).
PMID: 32764691
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Xu et al. (2020-01-01), Understanding the regulatory mechanisms of endometrial cells on activities of endometrial mesenchymal stem-like cells during menstruation Stem Cell Res Ther, 11(1):239.
PMID: 32552749
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Wei et al. (2020-01-01), Notch signalling drives synovial fibroblast identity and arthritis pathology Nature, 582(7811):259-264.
PMID: 32499639
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Grosswendt et al. (2020-01-01), Epigenetic regulator function through mouse gastrulation Nature, 584(7819):102-108.
PMID: 32728215
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

O McGinn et al. (2020-01-01), Cytokeratin 5 alters beta-catenin dynamics in breast cancer cells Oncogene, 39(12):2478-2492.
PMID: 31988452
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

NY Loh et al. (2020-01-01), RSPO3 impacts body fat distribution and regulates adipose cell biology in vitro Nat Commun, 11(1):2797.
PMID: 32493999
Species: Human
Sample Types: Organoids
Applications: Bioassay

T Kijima et al. (2019-01-01), Three-Dimensional Organoids Reveal Therapy Resistance of Esophageal and Oropharyngeal Squamous Cell Carcinoma Cells Cell Mol Gastroenterol Hepatol, 7(1):73-91.
PMID: 30510992
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Yu et al. (2019-01-01), ZNF326 promotes malignant phenotype of glioma by up-regulating HDAC7 expression and activating Wnt pathway J. Exp. Clin. Cancer Res., 38(1):40.
PMID: 30691485
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Chesnokova et al. (2019-01-01), Excess growth hormone suppresses DNA damage repair in epithelial cells JCI Insight, 4(3).
PMID: 30728323
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Noguchi et al. (2019-01-01), Induction of Expandable Tissue-Specific Progenitor Cells from Human Pancreatic Tissue through Transient Expression of Defined Factors Mol Ther Methods Clin Dev, 13(0):243-252.
PMID: 30828587
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Götzel et al. (2019-01-01), In-depth characterization of the Wnt-signaling/?-catenin pathway in an in vitro model of Barrett's sequence BMC Gastroenterol, 19(1):38.
PMID: 30841855
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

O Lyros et al. (2019-01-01), Dickkopf-1 (DKK1) promotes tumor growth via Akt-phosphorylation and independently of Wnt-axis in Barrett's associated esophageal adenocarcinoma Am J Cancer Res, 9(2):330-346.
PMID: 30906632
Species: Human
Sample Types: Whole Cells

X Wang et al. (2019-01-01), Point mutations in the PDX1 transactivation domain impair human ?-cell development and function Mol Metab, 0(0).
PMID: 30930126
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

P Shooshtari et al. (2019-01-01), Gfi1b regulates the level of Wnt/?-catenin signaling in hematopoietic stem cells and megakaryocytes Nat Commun, 10(1):1270.
PMID: 30894540
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Colunga et al. (2019-01-01), Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair Cell Rep, 26(10):2566-2579.e10.
PMID: 30840882
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Liu et al. (2019-01-01), An integrated chromatin accessibility and transcriptome landscape of human pre-implantation embryos Nat Commun, 10(1):364.
PMID: 30664750
Species: Fish - Oryzias latipes (Medaka/Rice Fish)
Sample Types: In Vivo
Applications: In Vivo

K Abe et al. (2019-01-01), Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite Cell Rep, 27(3):928-939.e4.
PMID: 30995487
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Kaiser et al. (2019-01-01), WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis Nat Commun, 10(1):1498.
PMID: 30940800
Species: Human
Sample Types: Organoids
Applications: Bioassay

Z Yan et al. (2019-01-01), Inhibition of ERK1/2 in cancer-associated pancreatic stellate cells suppresses cancer-stromal interaction and metastasis J. Exp. Clin. Cancer Res., 38(1):221.
PMID: 31133044
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Wang et al. (2019-01-01), Enhanced chondrogenesis from human embryonic stem cells Stem Cell Res, 39(0):101497.
PMID: 31326745
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

CP Nielsen et al. (2019-01-01), USP9X Deubiquitylates DVL2 to Regulate WNT Pathway Specification Cell Rep, 28(4):1074-1089.e5.
PMID: 31340145
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Y Wen et al. (2019-01-01), COL4A2 in the tissue-specific extracellular matrix plays important role on osteogenic differentiation of periodontal ligament stem cells Theranostics, 9(15):4265-4286.
PMID: 31285761
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CL Hammond et al. (2019-01-01), Proton pump inhibitors attenuate myofibroblast formation associated with thyroid eye disease through the aryl hydrocarbon receptor PLoS ONE, 14(9):e0222779.
PMID: 31536596
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AG Hawkins et al. (2019-01-01), Microenvironmental Factors Drive Tenascin C and Src Cooperation to Promote Invadopodia Formation in Ewing Sarcoma Neoplasia, 21(10):1063-1072.
PMID: 31521948
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Tonon et al. (2019-01-01), In vitro metabolic zonation through oxygen gradient on a chip Sci Rep, 9(1):13557.
PMID: 31537830
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

S Akbari et al. (2019-01-01), Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling Stem Cell Reports, 13(4):627-641.
PMID: 31522975
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Praça et al. (2019-01-01), Derivation of Brain Capillary-like Endothelial Cells from Human Pluripotent Stem Cell-Derived Endothelial Progenitor Cells Stem Cell Reports, 13(4):599-611.
PMID: 31495714
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Lungova et al. (2019-01-01), Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure Nat Commun, 10(1):4161.
PMID: 31551422
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KB Jung et al. (2019-01-01), Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells J Clin Med, 8(7).
PMID: 31277507
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Zheng et al. (2019-01-01), Controlled modelling of human epiblast and amnion development using stem cells Nature, 573(7774):421-425.
PMID: 31511693
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

RR Stine et al. (2019-01-01), PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism Cell Stem Cell, 0(0).
PMID: 31564549
Species: Porcine
Sample Types: Whole Tissue
Applications: Tissue Culture

AP Singh et al. (2019-01-01), Enteroendocrine progenitor cell enriched miR-7 regulates intestinal epithelial proliferation in an Xiap-dependent manner Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 31756561
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KH Grinnemo et al. (2019-01-01), Immunomodulatory effects of interferon-gamma on human fetal cardiac mesenchymal stromal cells Stem Cell Res Ther, 10(1):371.
PMID: 31801632
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

R Eyre et al. (2019-01-01), Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling Nat Commun, 10(1):5016.
PMID: 31676788
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Z Ye et al. (2019-01-01), Wnt Glycation Inhibits Canonical Signaling Cells, 8(11).
PMID: 31731544
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Ferrer-May et al. (2019-01-01), Vitamin D and Wnt3A have additive and partially overlapping modulatory effects on gene expression and phenotype in human colon fibroblasts Sci Rep, 9(1):8085.
PMID: 31147591
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Huang et al. (2019-01-01), Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis Cell Stem Cell, 0(0).
PMID: 31204176
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Aripaka et al. (2019-01-01), TRAF6 function as a novel co-regulator of Wnt3a target genes in prostate cancer EBioMedicine, 0(0).
PMID: 31262711
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Zhao et al. (2019-01-01), E2A attenuates tumor-initiating capacity of colorectal cancer cells via the Wnt/beta-catenin pathway J. Exp. Clin. Cancer Res., 38(1):276.
PMID: 31234887
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Sarkar et al. (2018-01-01), Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity Inï¿½Vitro Cell Stem Cell, 22(5):684-697.e9.
PMID: 29727680
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Q Li et al. (2018-01-01), A simple and scalable hydrogel-based system for culturing protein-producing cells PLoS ONE, 13(1):e0190364.
PMID: 29293594
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

XG Li et al. (2018-01-01), LGR5 and BMI1 Increase Pig Intestinal Epithelial Cell Proliferation by Stimulating WNT/?-Catenin Signaling Int J Mol Sci, 19(4).
PMID: 29601474
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

HK Lee et al. (2018-01-01), Ubiquitylation and degradation of adenomatous polyposis coli by MKRN1 enhances Wnt/?-catenin signaling Oncogene, 0(0).
PMID: 29713058
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Szemes et al. (2018-01-01), Wnt Signalling Drives Context-Dependent Differentiation or Proliferation in Neuroblastoma Neoplasia, 20(4):335-350.
PMID: 29505958
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

HJ Lee et al. (2018-01-01), Cholesterol Esterification Inhibition Suppresses Prostate Cancer Metastasis by Impairing the Wnt/?-catenin Pathway Mol. Cancer Res., 0(0).
PMID: 29545473
Species: Human
Sample Types: Organoids
Applications: Bioassay

D Garnier et al. (2018-01-01), Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system Sci Rep, 8(1):8222.
PMID: 29844473
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BN Nakamura et al. (2018-01-01), A20 regulates canonical wnt-signaling through an interaction with RIPK4 PLoS ONE, 13(5):e0195893.
PMID: 29718933
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Amin et al. (2018-01-01), Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like Phenotype Cell Transplant, 27(2):285-298.
PMID: 29637816
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Russo et al. (2018-01-01), Reliance upon ancestral mutations is maintained in colorectal cancers that heterogeneously evolve during targeted therapies Nat Commun, 9(1):2287.
PMID: 29895949
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Li et al. (2018-01-01), Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 0(0):9636897187803.
PMID: 29895168
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Condello et al. (2018-01-01), Tissue transglutaminase regulates interactions between ovarian cancer stem cells and the tumor niche Cancer Res., 0(0).
PMID: 29510995
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Wang et al. (2018-01-01), Genome-wide analysis of PDX1 target genes in human pancreatic progenitors Mol Metab, 0(0).
PMID: 29396371
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Kokabu et al. (2018-01-01), BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling FEBS Open Bio, 8(2):168-176.
PMID: 29435407
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Sui et al. (2018-01-01), IKK? is a ?-catenin kinase that regulates mesenchymal stem cell differentiation JCI Insight, 3(2).
PMID: 29367460
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Y Zhao et al. (2018-01-01), An essential role for Wnt/?-catenin signaling in mediating hypertensive heart disease Sci Rep, 8(1):8996.
PMID: 29895976
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H He et al. (2018-01-01), The Wnt-?-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells Oncotarget, 9(20):15239-15251.
PMID: 29632640
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

E Magro-Lope et al. (2018-01-01), Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids Stem Cell Res Ther, 9(1):186.
PMID: 29996941
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Yamada et al. (2018-01-01), Tributyltin Inhibits Neural Induction of Human Induced Pluripotent Stem Cells Sci Rep, 8(1):12155.
PMID: 30108368
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Ida et al. (2018-01-01), Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors Stem Cell Res Ther, 9(1):277.
PMID: 30359326
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Li et al. (2018-01-01), A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family PLoS ONE, 13(12):e0208110.
PMID: 30540809
Species: Human
Sample Types: Organoids
Applications: Bioassay

C Bonamy et al. (2018-01-01), Expression of the human antimicrobial peptide ?-defensin-1 is repressed by the EGFR-ERK-MYC axis in colonic epithelial cells Sci Rep, 8(1):18043.
PMID: 30575780
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

BA Brown et al. (2018-01-01), Aging differentially modulates the Wnt pro-survival signalling pathways in vascular smooth muscle cells Aging Cell, 0(0):e12844.
PMID: 30548452
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Q Peng et al. (2018-01-01), EPH receptor A2 governs a feedback loop that activates Wnt/?-catenin signaling in gastric cancer Cell Death Dis, 9(12):1146.
PMID: 30451837
Species: Human
Sample Types: Cell Lysates
Applications: Immunoassay Development

K Jeon et al. (2018-01-01), GLIS3 Transcriptionally Activates WNT Genes to Promote Differentiation of Human Embryonic Stem Cells into Posterior Neural Progenitors Stem Cells, 0(0).
PMID: 30376208
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

R Diacou et al. (2018-01-01), Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation Cell Rep, 25(9):2510-2523.e4.
PMID: 30485816
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KE Worley et al. (2018-01-01), Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation Stem Cells Int, 2018(0):1848605.
PMID: 30627170
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Cha et al. (2018-01-01), Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/?-Catenin Signaling Cell Rep, 25(3):571-584.e5.
PMID: 30332639
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Ogorodniko et al. (2018-01-01), Transcriptome 3'end organization by PCF11 links alternative polyadenylation to formation and neuronal differentiation of neuroblastoma Nat Commun, 9(1):5331.
PMID: 30552333
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Michael J Workman et al. (2018-01-01), Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips. Cellular and Molecular Gastroenterology and Hepatology, 0(0):2352-345X.
PMID: 29930984
Species: Human
Sample Types: Organoids
Applications: Bioassay

E Sechet et al. (2018-01-01), Natural molecules induce and synergize to boost expression of the human antimicrobial peptide ?-defensin-3 Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 30275324
Species: Human
Sample Types: Whole Cells

AG Hawkins et al. (2018-01-01), The Ewing sarcoma secretome and its response to activation of Wnt/beta-catenin signaling Mol. Cell Proteomics, 0(0).
PMID: 29386236
Species: Human
Sample Types: Whole Cells
Applications: Stimulation

S Lamprecht et al. (2018-01-01), PBX3 is part of an EMT regulatory network and indicates poor outcome in colorectal cancer Clin. Cancer Res., 0(0).
PMID: 29391352
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

J Gillespie et al. (2018-01-01), TGF? activation primes canonical Wnt signaling through the downregulation of AXIN2 Arthritis rheumatology (Hoboken, N.J.), 2018;0(0).
PMID: 29409120
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

LA Ahrens et al. (2017-01-01), Gelatin device for the delivery of growth factors involved in endochondral ossification PLoS ONE, 12(4):e0175095.
PMID: 28380024
Species: Mouse
Sample Types: Whole Embryo
Applications: Bioassay

K Minegishi et al. (2017-01-01), A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking Dev. Cell, 40(5):439-452.e4.
PMID: 28292423
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Wen et al. (2017-01-01), Up-regulated NRIP2 in colorectal cancer initiating cells modulates the Wnt pathway by targeting ROR? Mol. Cancer, 16(1):20.
PMID: 28137278
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Asai et al. (2017-01-01), Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells Development, 144(6):1056-1064.
PMID: 28275009
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Yang et al. (2017-01-01), Generation of Human Liver Chimeric Mice with Hepatocytes from Familial Hypercholesterolemia Induced Pluripotent Stem Cells Stem Cell Reports, 0(0).
PMID: 28262545
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

N Yucer et al. (2017-01-01), Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium Sci Rep, 7(1):10741.
PMID: 28878359
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Jang et al. (2017-01-01), WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells Biochem. Biophys. Res. Commun, 0(0).
PMID: 28137581
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

PC Sanchez-Di et al. (2017-01-01), Ubiquitin carboxyl-terminal esterase L1 (UCHL1) is associated with stem-like cancer cell functions in pediatric high-grade glioma PLoS ONE, 12(5):e0176879.
PMID: 28472177
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SJ Zhao et al. (2017-01-01), SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/?-catenin signaling through stabilization of the WNT-receptor complex Oncogene, 0(0).
PMID: 29084211
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

H Xiao et al. (2017-01-01), A Novel Positive Feedback Loop Between NTSR1 and Wnt/?-Catenin Contributes to Tumor Growth of Glioblastoma Cell. Physiol. Biochem., 43(5):2133-2142.
PMID: 29065410
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

I Savvidou et al. (2017-01-01), Beta-catenin inhibitor BC2059 is efficacious as monotherapy or in combination with proteasome inhibitor bortezomib in multiple myeloma Mol. Cancer Ther., 0(0).
PMID: 28500235
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Uribe-Etxe et al. (2017-01-01), Notch/Wnt cross-signalling regulates stemness of dental pulp stem cells through expression of neural crest and core pluripotency factors Eur Cell Mater, 34(0):249-270.
PMID: 29092089
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Montacir et al. (2017-01-01), The N-glycome of embryonic stem cells and thereof differentiated hepatic cells Chembiochem, 0(0).
PMID: 28370937
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Low et al. (2017-01-01), Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System Stem Cell Reports, 0(0).
PMID: 28457888
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JG Camp et al. (2017-01-01), Multilineage communication regulates human liver bud development from pluripotency Nature, 0(0).
PMID: 28614297
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Li et al. (2017-01-01), AKAP2 is upregulated in ovarian cancer, and promotes growth and migration of cancer cells Mol Med Rep, 0(0).
PMID: 28849175
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Jang et al. (2017-01-01), LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974 Sci Rep, 7(0):41612.
PMID: 28128299
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

H Isshiki et al. (2017-01-01), Establishment of a refined culture method for rat colon organoids Biochem. Biophys. Res. Commun., 0(0).
PMID: 28559141
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Premarathn et al. (2017-01-01), Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors Sci Rep, 7(1):8109.
PMID: 28808228
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Choudhury et al. (2017-01-01), Patient-specific hepatocyte-like cells derived from induced pluripotent stem cells model pazopanib-mediated hepatotoxicity Sci Rep, 7(0):41238.
PMID: 28120901
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

XH Jiang et al. (2017-01-01), Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/?-catenin pathway BMC Microbiol., 17(1):124.
PMID: 28545480
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Miura et al. (2017-01-01), Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming Cell Stem Cell, 0(0).
PMID: 28943029
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

DS Varghese et al. (2017-01-01), Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro Stem Cells Int, 2017(0):2451927.
PMID: 29147115
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Ou et al. (2017-01-01), The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells Biochem. Biophys. Res. Commun., 487(2):457-463.
PMID: 28431932
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Q Gu et al. (2017-01-01), Accreditation of Biosafe Clinical-Grade Human Embryonic Stem Cells According to Chinese Regulations Stem Cell Reports, 0(0).
PMID: 28506532
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

IT Cho et al. (2017-01-01), Aristaless Related Homeobox (ARX) Interacts with ?-Catenin, BCL9, and P300 to Regulate Canonical Wnt Signaling PLoS ONE, 12(1):e0170282.
PMID: 28103279
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JJ Kathiriya et al. (2017-01-01), Galectin-1 inhibition attenuates profibrotic signaling in hypoxia-induced pulmonary fibrosis Cell Death Discov, 3(0):17010.
PMID: 28417017
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Kishimoto et al. (2017-01-01), Wnt/?-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells PLoS ONE, 12(7):e0182051.
PMID: 28750046
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Messéant et al. (2017-01-01), Wnts contribute to neuromuscular junction formation through distinct signaling pathways Development, 0(0).
PMID: 28348167
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

ML Xu et al. (2017-01-01), Wnt3a Induces the Expression of Acetylcholinesterase during Osteoblast Differentiation via the Runx2 Transcription Factor J. Biol. Chem., 0(0).
PMID: 28607150
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CY Janda et al. (2017-01-01), Surrogate Wnt agonists that phenocopy canonical Wnt and ?-catenin signalling Nature, 0(0).
PMID: 28467818
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Nakamura et al. (2017-01-01), Age-related increase in Wnt inhibitor causes a senescence-like phenotype in human cardiac stem cells Biochem. Biophys. Res. Commun., 0(0).
PMID: 28435069
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JA Heslop et al. (2017-01-01), Donor-Dependent and Other Nondefined Factors Have Greater Influence on the Hepatic Phenotype Than the Starting Cell Type in Induced Pluripotent Stem Cell Derived Hepatocyte-Like Cells Stem Cells Transl Med, 6(5):1321-1331.
PMID: 28456008
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Monteagudo et al. (2017-01-01), DOT1L safeguards cartilage homeostasis and protects against osteoarthritis Nat Commun, 8(0):15889.
PMID: 28627522
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KB McCauley et al. (2017-01-01), Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling Cell Stem Cell, 0(0).
PMID: 28366587
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

DM Velázquez et al. (2017-01-01), Dishevelled stability is positively regulated by PKC?-mediated phosphorylation induced by Wnt agonists Cell. Signal., 35(0):107-117.
PMID: 28366812
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Lucendo-Vi et al. (2017-01-01), Modelling foetal exposure to maternal smoking using hepatoblasts from pluripotent stem cells Arch. Toxicol., 0(0).
PMID: 28510779
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Zeng et al. (2017-01-01), Wnt/?-catenin signaling contributes to tumor malignancy and is targetable in gastrointestinal stromal tumor Mol. Cancer Ther., 0(0).
PMID: 28611108
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Yukihiro Saito et al. (2017-01-01), Suppression of Wnt Signaling and Osteogenic Changes in Vascular Smooth Muscle Cells by Eicosapentaenoic Acid. Nutrients, 0(0):2072-6643.
PMID: 28796175
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

N Arora et al. (2017-01-01), A process engineering approach to increase organoid yield Development, 144(6):1128-1136.
PMID: 28174251
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28028233
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Activation of endothelial ?-catenin signaling induces heart failure Sci Rep, 6(0):25009.
PMID: 27146149
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/?-catenin defects in neuronal cholesterol synthesis phenotypes Nat. Med., 22(4):388-96.
PMID: 26998835
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), TNIK inhibition abrogates colorectal cancer stemness Nat Commun, 7(0):12586.
PMID: 27562646
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), The AKAP GSKIP regulates beta-catenin through both its interactions with PKA and GSK3beta J Biol Chem, 0(0).
PMID: 27484798
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells Sci Rep, 6(0):36795.
PMID: 27827428
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Elena Neumann (2016-01-01), Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts Matrix Biol., 0(0).
PMID: 27884778
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Karthikeyan Mythreye (2016-01-01), Altering the Proteoglycan State of Transforming Growth Factor ? Type III Receptor (T?RIII)/Betaglycan Modulates Canonical Wnt/?-Catenin Signaling J. Biol. Chem., 291(49):25716-25728.
PMID: 27784788
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ronald A DePinho (2016-01-01), Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth Cell, 167(5):1281-1295.e18.
PMID: 27863244
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Quantifying ?-catenin subcellular dynamics and cyclin D1 mRNA transcription during Wnt signaling in single living cells Elife, 5(0).
PMID: 27879202
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Richard P Phipps (2016-01-01), The Aryl Hydrocarbon Receptor and Its Ligands Inhibit Myofibroblast Formation and Activation: Implications for Thyroid Eye Disease Am. J. Pathol., 0(0).
PMID: 27842700
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Hoeke A Baarsma (2016-01-01), Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD J. Exp. Med, 0(0).
PMID: 27979969
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation Stem Cell Res, 17(2):222-227.
PMID: 27591478
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

L J Spicer (2016-01-01), G protein-coupled receptor 34 in ovarian granulosa cells of cattle: changes during follicular development and potential functional implications Domest. Anim. Endocrinol, 59(0):90-99.
PMID: 28040605
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Persad et al. (2016-01-01), Active ?-catenin is regulated by the PTEN/PI3 kinase pathway: a role for protein phosphatase PP2A Genes Cancer, 7(12):368-382.
PMID: 28191283
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Growth hormone is permissive for neoplastic colon growth Proc Natl Acad Sci USA, 0(0).
PMID: 27226307
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of the YAP/TAZ transcriptional coactivator WBP2 Cancer Res, 0(0).
PMID: 27578003
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Anthelmintic niclosamide disrupts the interplay of p65 and FOXM1/?-catenin and eradicates leukemia stem cells in chronic myelogenous leukemia Clin Cancer Res, 0(0).
PMID: 27492973
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gui-Li Huang (2016-01-01), Oncogenic retinoic acid receptor ? promotes human colorectal cancer growth through simultaneously regulating p21 transcription and GSK3?/?-catenin signaling Cancer Lett, 388(0):118-129.
PMID: 27932034
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration Stem Cells Transl Med, 5(5):694-702.
PMID: 26987352
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Junhua Wang et al. (2016-01-01), Icariin attenuates titanium-particle inhibition of bone formation by activating the Wnt/?-catenin signaling pathway in vivo and in vitro. Scientific Reports, 0(0):2045-2322.
PMID: 27029606
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

James M et al. (2015-01-01), Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note. PLoS ONE, 10(2):e0117776.
PMID: 25658706
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Viale-Bouroncle S et al. (2015-01-01), A protein kinase A (PKA)/beta-catenin pathway sustains the BMP2/DLX3-induced osteogenic differentiation in dental follicle cells (DFCs). Cell Signal, 27(3):598-605.
PMID: 25530217
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Grigson E et al. (2015-01-01), Canonical Wnt pathway inhibitor ICG-001 induces cytotoxicity of multiple myeloma cells in Wnt-independent manner. PLoS ONE, 10(1):e0117693.
PMID: 25635944
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tesfay L et al. (2015-01-01), Hepcidin regulation in prostate and its disruption in prostate cancer. Cancer Res, 75(11):2254-63.
PMID: 25858146
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Williams K et al. (2015-01-01), Focal adhesion kinase and Wnt signaling regulate human ductal carcinoma in situ stem cell activity and response to radiotherapy. Stem Cells, 33(2):327-41.
PMID: 25187396
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Huang T et al. (2015-01-01), A Regulatory Network Involving beta-Catenin, e-Cadherin, PI3k/Akt, and Slug Balances Self-Renewal and Differentiation of Human Pluripotent Stem Cells In Response to Wnt Signaling. Stem Cells, 33(5):1419-33.
PMID: 25538040
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Xue J et al. (2015-01-01), Tumour suppressor TRIM33 targets nuclear beta-catenin degradation. Nat Commun, 6(0):6156.
PMID: 25639486
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Handorf A et al. (2015-01-01), Endogenously produced Indian Hedgehog regulates TGFbeta-driven chondrogenesis of human bone marrow stromal/stem cells. Stem Cells Dev, 24(8):995-1007.
PMID: 25519748
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tamminen K et al. (2015-01-01), Intestinal Commitment and Maturation of Human Pluripotent Stem Cells Is Independent of Exogenous FGF4 and R-spondin1. PLoS ONE, 10(7):e0134551.
PMID: 26230325
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Maubant S et al. (2015-01-01), Transcriptome analysis of wnt3a-treated triple-negative breast cancer cells. PLoS ONE, 10(4):e0122333.
PMID: 25848952
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wong et al. (2015-01-01), Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. Nat Protoc, 10(3):363-81.
PMID: 25654755
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kuhnl A et al. (2015-01-01), Downregulation of the Wnt inhibitor CXXC5 predicts a better prognosis in acute myeloid leukemia. Blood, 125(19):2985-94.
PMID: 25805812
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zanni R et al. (2015-01-01), Novel cancer chemotherapy hits by molecular topology: dual Akt and Beta-catenin inhibitors. PLoS ONE, 10(4):e0124244.
PMID: 25910265
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tsankov et al. (2015-01-01), Transcription factor binding dynamics during human ES cell differentiation. Nature, 518(7539):344-9.
PMID: 25693565
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Iyer D et al. (2015-01-01), Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells. Development, 142(8):1528-41.
PMID: 25813541
Peng H et al. (2015-01-01), microRNA-103/107 Family Regulates Multiple Epithelial Stem Cell Characteristics. Stem Cells, 33(5):1642-56.
PMID: 25639731
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chang B et al. (2015-01-01), Epsin is required for Dishevelled stability and Wnt signalling activation in colon cancer development. Nat Commun, 6(0):6380.
PMID: 25871009
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kim M et al. (2015-01-01), Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis. PLoS ONE, 10(2):e0118670.
PMID: 25706534
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Huang S et al. (2015-01-01), The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells. Nat Protoc, 10(3):413-25.
PMID: 25654758
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Pezzolla D et al. (2015-01-01), Resveratrol ameliorates the maturation process of beta-cell-like cells obtained from an optimized differentiation protocol of human embryonic stem cells. PLoS ONE, 10(3):e0119904.
PMID: 25774684
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Listerman I et al. (2014-01-01), An investigation of the effects of the core protein telomerase reverse transcriptase on Wnt signaling in breast cancer cells. Mol Cell Biol, 34(2):280-9.
PMID: 24216762
Cnossen W et al. (2014-01-01), Whole-exome sequencing reveals LRP5 mutations and canonical Wnt signaling associated with hepatic cystogenesis. Proc Natl Acad Sci U S A, 111(14):5343-8.
PMID: 24706814
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Guezguez A et al. (2014-01-01), Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Exp Cell Res, 322(2):355-64.
PMID: 24534551
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sundaram S et al. (2014-01-01), Tissue-engineered vascular grafts created from human induced pluripotent stem cells. Stem Cells Transl Med, 3(12):1535-43.
PMID: 25378654
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

van den Bosch M et al. (2014-01-01), Canonical Wnt signaling skews TGF-beta signaling in chondrocytes towards signaling via ALK1 and Smad 1/5/8. Cell Signal, 26(5):951-8.
PMID: 24463008
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Nakamura I et al. (2014-01-01), Brivanib attenuates hepatic fibrosis in vivo and stellate cell activation in vitro by inhibition of FGF, VEGF and PDGF signaling. PLoS ONE, 9(4):e92273.
PMID: 24710173
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Meinhardt G et al. (2014-01-01), Wnt-dependent T-cell factor-4 controls human etravillous trophoblast motility. Endocrinology, 155(5):1908-20.
PMID: 24605829
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hirata et al. (2014-01-01), Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation. Nat Commun, 5(0):4806.
PMID: 25254944
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Peng X et al. (2014-01-01), Wnt/beta-catenin signaling regulates the proliferation and differentiation of mesenchymal progenitor cells through the p53 pathway. PLoS ONE, 9(5):e97283.
PMID: 24819053
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Tsai T et al. (2014-01-01), Pigment epithelium-derived factor 34-mer peptide prevents liver fibrosis and hepatic stellate cell activation through down-regulation of the PDGF receptor. PLoS ONE, 9(4):e95443.
PMID: 24763086
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Pethe et al. (2014-01-01), Polycomb group protein expression during differentiation of human embryonic stem cells into pancreatic lineage in vitro. BMC Cell Biol, 15(0):18.
PMID: 24885493
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hwang Y et al. (2014-01-01), WNT3A promotes myogenesis of human embryonic stem cells and enhances in vivo engraftment. Sci Rep, 4(0):5916.
PMID: 25084050
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lin P et al. (2014-01-01), A synthetic peptide-acrylate surface for production of insulin-producing cells from human embryonic stem cells. Stem Cells Dev, 23(4):372-9.
PMID: 24083371
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ekstrom E et al. (2014-01-01), WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells. Mol Cancer, 13(0):88.
PMID: 24766647
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Jin R et al. (2014-01-01), The metastasis suppressor NDRG1 modulates the phosphorylation and nuclear translocation of beta-catenin through mechanisms involving FRAT1 and PAK4. J Cell Sci, 127(0):3116-30.
PMID: 24829151
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lin et al. (2014-01-01), Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells. Stem Cell Reports, 2(2):189-204.
PMID: 24527393
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hinton A et al. (2014-01-01), sRNA-seq analysis of human embryonic stem cells and definitive endoderm reveals differentially expressed microRNAs and novel IsomiRs with distinct targets. Stem Cells, 32(9):2360-72.
PMID: 24805944
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Todaro M et al. (2014-01-01), CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis. Cell Stem Cell, 14(3):342-56.
PMID: 24607406
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Emmrich S et al. (2014-01-01), miR-99a/100~125b tricistrons regulate hematopoietic stem and progenitor cell homeostasis by shifting the balance between TGFbeta and Wnt signaling. Genes Dev, 28(8):858-74.
PMID: 24736844
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Carmon K et al. (2014-01-01), RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. Proc Natl Acad Sci U S A, 111(13):E1221-9.
PMID: 24639526
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cechin S et al. (2014-01-01), Influence of in vitro and in vivo oxygen modulation on beta cell differentiation from human embryonic stem cells. Stem Cells Transl Med, 3(3):277-89.
PMID: 24375542
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Massumi M et al. (2014-01-01), Efficient programming of human eye conjunctiva-derived induced pluripotent stem (ECiPS) cells into definitive endoderm-like cells. Exp Cell Res, 322(1):51-61.
PMID: 24434355
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lam A et al. (2014-01-01), Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers. J Am Soc Nephrol, 25(6):1211-25.
PMID: 24357672
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chen E et al. (2014-01-01), Glycogen synthase kinase 3 inhibitors induce the canonical WNT/beta-catenin pathway to suppress growth and self-renewal in embryonal rhabdomyosarcoma. Proc Natl Acad Sci U S A, 111(14):5349-54.
PMID: 24706870
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Aloia L et al. (2014-01-01), Zrf1 is required to establish and maintain neural progenitor identity. Genes Dev, 28(2):182-97.
PMID: 24449271
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kang et al. (2014-01-01), Generation of bladder urothelium from human pluripotent stem cells under chemically defined serum- and feeder-free system. Int J Mol Sci, 15(5):7139-57.
PMID: 24776760
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kang et al. (2014-01-01), Differentiation of human pluripotent stem cells into nephron progenitor cells in a serum and feeder free system. PLoS ONE, 9(4):e94888.
PMID: 24728509
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Dhamdhere et al. (2014-01-01), Drugging a stem cell compartment using Wnt3a protein as a therapeutic. PLoS ONE, 9(1):e83650.
PMID: 24400074
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Jabaji Z et al. (2014-01-01), Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium. PLoS ONE, 9(9):e107814.
PMID: 25222024
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fuerer C et al. (2014-01-01), Nodal.Gdf1 heterodimers with bound prodomains enable serum-independent nodal signaling and endoderm differentiation. J Biol Chem, 289(25):17854-71.
PMID: 24798330
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hillesheim A et al. (2014-01-01), beta-catenin promotes the type I IFN synthesis and the IFN-dependent signaling response but is suppressed by influenza A virus-induced RIG-I/NF-kappaB signaling. Cell Commun Signal, 12(0):29.
PMID: 24767605
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kilander M et al. (2014-01-01), Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 26(9):1943-9.
PMID: 24873871
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Ao et al. (2014-01-01), High efficient differentiation of functional hepatocytes from porcine induced pluripotent stem cells. PLoS ONE, 9(6):e100417.
PMID: 24949734
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lee J et al. (2014-01-01), Interleukin-1beta-induced Wnt5a enhances human corneal endothelial cell migration through regulation of Cdc42 and RhoA. Mol Cell Biol, 34(18):3535-45.
PMID: 25022753
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cecchelli R et al. (2014-01-01), A stable and reproducible human blood-brain barrier model derived from hematopoietic stem cells. PLoS ONE, 9(6):e99733.
PMID: 24936790
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tsai H et al. (2014-01-01), Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE, 9(8):e104937.
PMID: 25170755
Wu C et al. (2014-01-01), RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun, 5(0):3149.
PMID: 24476626
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

Skoczek D et al. (2014-01-01), Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium. J Immunol, 193(1):439-51.
PMID: 24907348
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hernandez-Maqueda J et al. (2013-01-01), Protein kinase C delta negatively modulates canonical Wnt pathway and cell proliferation in colon tumor cell lines. PLoS ONE, 8(3):e58540.
PMID: 23520519
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rasmussen N et al. (2013-01-01), Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer. J Biol Chem, 288(36):26301-10.
PMID: 23893409
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Goto T et al. (2013-01-01), IQGAP1 protein regulates nuclear localization of beta-catenin via importin-beta5 protein in Wnt signaling. J Biol Chem, 288(51):36351-60.
PMID: 24196961
Jiang Y et al. (2013-01-01), Activation of the Wnt pathway through AR79, a GSK3beta inhibitor, promotes prostate cancer growth in soft tissue and bone. Mol Cancer Res, 11(12):1597-610.
PMID: 24088787
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rezania A et al. (2013-01-01), Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo. Stem Cells, 31(11):2432-42.
PMID: 23897760
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2013-01-01), Cripto-1 enhances the canonical Wnt/ÃŽÂ²-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors. Cell Signal, 25(1):178-89.
PMID: 23022962
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Loh Y et al. (2013-01-01), The Wnt signalling pathway is upregulated in an in vitro model of acquired tamoxifen resistant breast cancer. BMC Cancer, 13(0):174.
PMID: 23547709
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Greer Y et al. (2013-01-01), Atypical protein kinase Ciota is required for Wnt3a-dependent neurite outgrowth and binds to phosphorylated dishevelled 2. J Biol Chem, 288(13):9438-46.
PMID: 23396968
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hannan et al. (2013-01-01), Generation of multipotent foregut stem cells from human pluripotent stem cells. Stem Cell Reports, 1(4):293-306.
PMID: 24319665
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Thatava T et al. (2013-01-01), Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells. Mol Ther, 21(1):228-39.
PMID: 23183535
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tahir S et al. (2013-01-01), Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer. Cancer Res, 73(6):1900-11.
PMID: 23302227
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012-01-01), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Cohen E et al. (2012-01-01), Wnt5a and Wnt11 are essential for second heart field progenitor development. Development, 139(11):1931-40.
PMID: 22569553
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Erceg S et al. (2012-01-01), Derivation of cerebellar neurons from human pluripotent stem cells. Curr Protoc Stem Cell Biol, 20(0):1H.51.
PMID: 22415839
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sui L et al. (2012-01-01), FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells. Biochem Biophys Res Commun, 426(3):380-5.
PMID: 22960178
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ichii et al. (2012-01-01), The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. Blood, 119(7):1683-92.
PMID: 22117039
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

Aad et al. (2012-01-01), The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems. Biol Reprod, 87(4):79.
PMID: 22811575
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Prabakar K et al. (2012-01-01), Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant, 21(6):1321-39.
PMID: 22195604
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kohler EE et al. (2011-01-01), NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis. Blood, 117(5):1761-9.
PMID: 21119109
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liu N et al. (2011-01-01), High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway. J. Bone Miner. Res., 26(9):2082-95.
PMID: 21638320
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Spence JR et al. (2011-01-01), Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 470(7332):105-9.
PMID: 21151107
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gudjonsson JE et al. (2010-01-01), Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 130(7):1849-59.
PMID: 20376066
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liu G et al. (2009-01-01), Canonical Wnts function as potent regulators of osteogenesis by human mesenchymal stem cells. J. Cell Biol., 185(1):67-75.
PMID: 19349579
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Henry C et al. (), Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion. Oncotarge6(37):40310-26.
PMID: 26515598

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Customer Reviews for Recombinant Human Wnt-3a Protein (25)

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Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: Cell migration/motility

Verified Customer | Posted 05/20/2023

wnt3a promote wound healing.
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 01/01/2022

wnt3a was used for the differentiation of cerebral organoid
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: CellProlif

Verified Customer | Posted 11/29/2021

avoid freezing and refreezing.
Recombinant Human Wnt-3a Protein

Name: Ricardo Malvicini

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 09/26/2021
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: CellProlif

Verified Customer | Posted 08/17/2021

200ng/ml wn3a CF can further promote the colony formation efficiency.
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 08/17/2021
Recombinant Human Wnt-3a Protein

Name: Mike van Nijmegen

Application: Enzymatic activity in vitro

Reason for Rating: excellent product, very repeated product, we bought it many times with different lot numbers

Verified Customer | Posted 08/06/2021
Recombinant Human Wnt-3a Protein

Name: Mike van Nijmegen

Application: CellProlif

Reason for Rating: This product works good.

Verified Customer | Posted 07/20/2021

Use the final concentration that is recommended, don't make the final concentration very low. Hek293SFT cells were treated with different concentrations of wnt3a, and topflash assay was performed to detect the luciferase activity of wnt3a.
Recombinant Human Wnt-3a Protein

Name: Ermali Aliaj

Application: Cell Culture

Verified Customer | Posted 01/02/2021
Recombinant Human Wnt-3a Protein

Name: Techung Lee

Application: Enzymatic activity in vitro

Verified Customer | Posted 11/06/2020

HepG2 cells were treated with 100 ng/ml Wnt3a for 2 days, following which the LDH activity of cell culture medium samples were assayed. The result shows that Wnt3a increased LDH release in HepG2 cells.
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: SDS-PAGE Control

Reason for Rating: Recombinant Wnt3a product was added to activate Wnt/ beta -catenin signaling pathway.

Verified Customer | Posted 02/16/2020
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Reason for Rating: This protein (200 ng/mL) works well for activating Wnt signaling as shown by Topflash luciferase activity.

Verified Customer | Posted 12/16/2018
Name: Mark Esposito

Application: In vitro bioactivity in cell culture

Reason for Rating: The product worked to activate Wnt signaling in breast cancer cells with a Wnt reporter, however performed less well than supernatants from L cell Wnt3a lines.

Verified Customer | Posted 10/27/2018

Recombinant Wnt3a product was added at 100 ng/mL for 2 days on 7TGC reporter cell lines. Activation was measured with GFP levels by Flow.
Name: Anonymous

Application: CellProlif

Verified Customer | Posted 10/17/2018
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 12/06/2017
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 06/07/2017
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: Cell migration/motility

Verified Customer | Posted 03/14/2017

Cell motility assay performed with MA-11 cells untreated (A) and treated (B) for 72 hours with R&D Recombinant Human Wnt-3a (#5036-WN/CF) at 250 ng/mL.
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 12/15/2016

We use for stem cell maintenance and differentiation.
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 12/15/2016

We use this product for our neuron stem cells studies.
Recombinant Human Wnt-3a Protein

Name: Francesco Marino

Application: In vitro bioactivity in cell culture

Reason for Rating: It works well.

Verified Customer | Posted 11/11/2016

I tested the activity of the recombinant protein in the HEK293 Top Flash Reporter cell lines. LiCl was used as a positive control.
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: Luciferase reporter activity (TOPFlash for Wnt activity) was performed in cultured cells.

Verified Customer | Posted 08/08/2016

Luciferase reporter activity by TOPFlash/FOPFlash for Wnt/B-catenin activity was performed in cultured cells. Cells were transfected for 48 hours with plasmid and treated with Wnt3a for 24 hours prior to performing Dual-Glo luciferase assay.
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Reason for Rating: We attempted to use the product to induce skeletal muscle differentiation in Mus musculus stem cells, but our results suggest differentiation to cardiomyocytes.

Verified Customer | Posted 05/13/2016
Recombinant Human Wnt-3a Protein

Name: Shireen Jayman

Application: CellProlif

Verified Customer | Posted 01/15/2016
Name: Anonymous

Application: SDS-PAGE Control

Reason for Rating: I have used Wnt3a routinely to stimulate primary cells in culture. I have obtained consistent results with reproducible data.

Verified Customer | Posted 01/13/2016
Recombinant Human Wnt-3a Protein

Name: Anonymous

Application: Media additive for protein or antibody production

Reason for Rating: This protein works very well for activating Wnt signaling as shown by B-catenin expression.

Verified Customer | Posted 01/13/2016

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FAQs for Recombinant Human Wnt-3a Protein

Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) at a different concentration than recommended?

We cannot guarantee the performance if the protein is reconstituted at a different concentration than recommended in the product datasheet. Please contact Technical Service if you have any questions or concerns about reconstitution.
Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) in a different buffer than recommended?

For best recovery, stability, and activity, the protein should be reconstituted in the buffer recommended on the product datasheet. Stability testing has been evaluated under these conditions; we can support the performance under these reconstitution conditions.

Recombinant Human Wnt-3a Protein

R&D Systems, Inc. a Bio-Techne Brand

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Formulation, Preparation and Storage

Carrier Free

5036-WN

Carrier

5036-WN/CF

Carrier Free

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Background: Wnt-3a

Citations for Recombinant Human Wnt-3a Protein (319)

Customer Reviews for Recombinant Human Wnt-3a Protein (25)

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Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein

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