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Recombinant Human Wnt-3a Protein Best Seller

Bio-Techne includes R&D Systems | Catalog # 5036-WN

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Carrier Free
5036-WN-010/CF
5036-WN-500/CF

Catalog #
Availability
Size / Price
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With Carrier
5036-WN-010
5036-WN-500

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein
Ser19-Lys352

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser19

Predicted Molecular Mass

37.4 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.
The ED50 for this effect is 5.00-25.0 ng/mL.

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
Optimal concentrations should be determined by each laboratory for each application.

Scientific Data Images for Recombinant Human Wnt-3a Protein

Recombinant Human Wnt-3a Protein Bioactivity

Recombinant Human Wnt-3a Protein Bioactivity

Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. The ED50 is 1.7-fold better with more than twice the maximum response compared to the top competitor's Wnt-3a.

Recombinant Human Wnt-3a Protein Lot to Lot Consistency

The lot-to-lot consistency of Recombinant Human Wnt-3a Protein (Catalog # 5036-WN) was assessed by testing the ability of three independent lots of the protein to induce alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. Each trace on the graph represents data obtained from Recombinant Human Wnt-3a from a different manufacturing run. The ED50 for this effect is 5.00-25.0 ng/mL.

iPSC-derived Human Intestinal Organoids Cultured using Recombinant Human Wnt-3a Protein.

iPSC-derived human intestinal organoids were cultured using Cultrex™ UltiMatrix RGF Basement Membrane Extract (BME001-05) and intestinal organoid culture medium, which includes Recombinant Human Wnt-3a (Catalog # 5036-WN), Recombinant Human EGF (236-EG), Recombinant Human Noggin (6057-NG), and Recombinant Human R-Spondin 1 (4645-RS), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; green) and a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (5748; blue).

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 5036-WN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 5036-WN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-3a

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta-catenin levels (2). As intracellular beta-catenin levels rise, beta-catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta-catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).

References

  1. Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  3. Korinek, V. et al. (1997) Science 275:1784.
  4. Chen, B. et al. (2009) Nat. Chem. Biol. 5:100.
  5. Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267.
  6. Takada, R. et al. (2006) Dev. Cell 11:791.
  7. Komekado, H. (2007) Genes Cells 12:521.
  8. Dunty Jr. W. C. et al. (2008) Development 135:85.
  9. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  10. Lee, S. M. et al. (2000) Development 127:457.
  11. Takada, S. et al. (1994) Genes Dev. 8:174.
  12. Willert, K. et al. (2003) Nature 423:6938.
  13. Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970.
  14. Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.

Long Name

Wingless-type MMTV Integration Site Family, Member 3a

Alternate Names

Wnt3a

Entrez Gene IDs

89780 (Human); 22416 (Mouse)

Gene Symbol

WNT3A

Additional Wnt-3a Products

Product Documents for Recombinant Human Wnt-3a Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Wnt-3a Protein

For research use only

Citations for Recombinant Human Wnt-3a Protein (345)

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Customer Reviews for Recombinant Human Wnt-3a Protein (27)

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  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/11/2023
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: MARIA PINNA
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 09/22/2023
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 05/20/2023
    wnt3a promote wound healing.
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 01/01/2022
    wnt3a was used for the differentiation of cerebral organoid
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 11/29/2021
    avoid freezing and refreezing.
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Ricardo Malvicini
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 09/26/2021
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 08/17/2021
    200ng/ml wn3a CF can further promote the colony formation efficiency.
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 08/17/2021
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Mike van Nijmegen
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 08/06/2021
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Mike van Nijmegen
    Application: Cell Proliferation
    Verified Customer | Posted 07/20/2021
    Use the final concentration that is recommended, don't make the final concentration very low. Hek293SFT cells were treated with different concentrations of wnt3a, and topflash assay was performed to detect the luciferase activity of wnt3a.
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Ermali Aliaj
    Application: Cell Culture
    Verified Customer | Posted 01/02/2021
  • Recombinant Human Wnt-3a Protein
    Name: Techung Lee
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 11/06/2020
    HepG2 cells were treated with 100 ng/ml Wnt3a for 2 days, following which the LDH activity of cell culture medium samples were assayed. The result shows that Wnt3a increased LDH release in HepG2 cells.
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 02/16/2020
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/16/2018
    Recombinant Human Wnt-3a Protein 5036-WN
  • Name: Mark Esposito
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/27/2018
    Recombinant Wnt3a product was added at 100 ng/mL for 2 days on 7TGC reporter cell lines. Activation was measured with GFP levels by Flow.
    Recombinant Human Wnt-3a Protein 5036-WN
  • Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 10/17/2018
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/06/2017
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/07/2017
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 03/14/2017
    Cell motility assay performed with MA-11 cells untreated (A) and treated (B) for 72 hours with R&D Recombinant Human Wnt-3a (#5036-WN/CF) at 250 ng/mL.
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 12/15/2016
    We use for stem cell maintenance and differentiation.
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 12/15/2016
    We use this product for our neuron stem cells studies.
  • Recombinant Human Wnt-3a Protein
    Name: Francesco Marino
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 11/11/2016
    I tested the activity of the recombinant protein in the HEK293 Top Flash Reporter cell lines. LiCl was used as a positive control.
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Luciferase reporter activity
    Verified Customer | Posted 08/08/2016
    Luciferase reporter activity by TOPFlash/FOPFlash for Wnt/B-catenin activity was performed in cultured cells. Cells were transfected for 48 hours with plasmid and treated with Wnt3a for 24 hours prior to performing Dual-Glo luciferase assay.
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 05/13/2016
    Recombinant Human Wnt-3a Protein 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Shireen Jayman
    Application: Cell Proliferation
    Verified Customer | Posted 01/15/2016
  • Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 01/13/2016
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Media additive for protein or antibody production
    Verified Customer | Posted 01/13/2016

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FAQs for Recombinant Human Wnt-3a Protein

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  • Q: Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) at a different concentration than recommended?    

    A: We cannot guarantee the performance if the protein is reconstituted at a different concentration than recommended in the product datasheet. Please contact Technical Service if you have any questions or concerns about reconstitution.

  • Q: Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) in a different buffer than recommended?

    A: For best recovery, stability, and activity, the protein should be reconstituted in the buffer recommended on the product datasheet. Stability testing has been evaluated under these conditions; we can support the performance under these reconstitution conditions.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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