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Recombinant Human/Mouse/Rat BMP-2 Protein

More economical E. coli option available
Graph showing dose-dependent bioactivity of Human BMP-2 protein
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With Carrier
355-BM-01M
355-BM-050
355-BM-100
355-BM-010

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Availability
Size / Price
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Carrier Free
355-BM-050/CF
355-BM-100/CF
355-BM-010/CF
355-BM-500/CF
355-BM-01M/CF

Key Product Details

Source: CHO
Accession #: P12643
Structure / Form: Disulfide-linked homodimer
Applications: Bioactivity

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Citations (161)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human/Mouse/Rat BMP-2 Protein include:

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Customer Reviews (10)

4.8 out of 5
10 customer ratings
5 stars
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  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Reason for Rating: I used it for osteoblasts differentiation, Arizarin staining.It keeps and promotes osteoblasts mineralization very well.
    Verified Customer | Posted 05/27/2021
    Recombinant Human/Mouse/Rat BMP-2 Protein Stem/Immune cell maintenance or differentiation  355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Reason for Rating: I used BMP2 to activate BMP reporter and it worked very well.
    Verified Customer | Posted 09/15/2020
    Recombinant Human/Mouse/Rat BMP-2 Protein In vitro bioactivity in cell culture 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: CellProlif
    Reason for Rating: Another great product made by R&D Systems. Always produces consistent and reliable results. Great as a control.
    Verified Customer | Posted 10/09/2019
    Recombinant Human/Mouse/Rat BMP-2 Protein Cell Proliferation 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Immunofluorescence for pSMAD 1,5,8 following BMP treatment
    Verified Customer | Posted 10/26/2018
    Recombinant Human/Mouse/Rat BMP-2 Protein Immunofluorescence for pSMAD 1,5,8 following BMP treatment 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Reason for Rating: Performed as expected to force differentiation of C2C12's to Osteoblasts
    Verified Customer | Posted 09/07/2017
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Verified Customer | Posted 05/03/2017
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: BMP-2 Delivery System
    Verified Customer | Posted 01/05/2017
    Recombinant Human/Mouse/Rat BMP-2 Protein BMP-2 Delivery System 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Reason for Rating: Materials performed as expected.
    Verified Customer | Posted 05/13/2016
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Reason for Rating: This protein performed exactly as expected.
    Verified Customer | Posted 01/15/2016
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Enzyme assay
    Sample Tested: C2C12 mouse myoblast cell line
    Verified Customer | Posted 10/26/2015
    ALP assay measuring osteogenic induction of C2C12 cells treated with BMP-2.
    Recombinant Human/Mouse/Rat BMP-2 Protein Enzyme assay 355-BM

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FAQs

  • The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

    Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

  • What is the half-life of a recombinant or natural protein in culture at 37 °C?

    R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

  • What is the recommended method for reconstitution of a lyophilized protein or antibody?

    Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.   

  • Are R&D Systems recombinant proteins and antibodies sterile?

    Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

  • What is the difference between the two Recombinant Human/Mouse/Rat BMP-2 Proteins (Catalog # 355-BM and 355-BEC)?

    Catalog # 355-BM is produced in a mammalian system using CHO cells and exhibits better activity in our routine QC bioassay, while Catalog # 355-BEC is produced in an E. coli expression system and is less expensive. Bioactivity of both Human/Mouse/Rat BMP-2 Proteins is measured in an alkaline phosphatase assay using by ATDC5 mouse chondrogenic cells. For 355-BM, the ED50 for this effect is 40-200 ng/mL, while for 355-BEC, the ED50 range is 80-480 ng/mL. We recommend reviewing our list of publications under the Citations tab for both 355-BM and 355-BEC to determine which of the two proteins is most suitable for your experiment.

  • What is the sequence homology between human, mouse and rat BMP-2

    Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2.

  • Does Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) have a histidine tag?

    Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) does not contain a histidine tag. The sequence of this protein contains Gln283-Arg396 aa based on Accession # P12643.

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.