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Recombinant Human/Mouse/Rat BMP-2 Protein

R&D Systems, Inc. a Bio-Techne Brand

More economical E. coli option available
Graph showing dose-dependent bioactivity of Human BMP-2 protein
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355-BM-050
355-BM-01M
355-BM-100
355-BM-010

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355-BM-500/CF
355-BM-100/CF
355-BM-01M/CF
355-BM-050/CF
355-BM-010/CF
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Key Product Details

Source: CHO
Accession #: P12643
Structure / Form: Disulfide-linked homodimer
Applications: Bioactivity

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Citations for Recombinant Human/Mouse/Rat BMP-2 Protein (178)

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Customer Reviews for Recombinant Human/Mouse/Rat BMP-2 Protein (11)

4.8 out of 5
11 customer ratings
5 stars
82%
4 stars
18%
3 stars
0%
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  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Reason for Rating: 50 ng/ml works well to induce osteoblast differentiation.
    Verified Customer | Posted 04/05/2022
    Recombinant Human/Mouse/Rat BMP-2 Protein Enzymatic activity in vitro 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: zeji du
    Application: Stem/Immune cell maintenance or differentiation
    Reason for Rating: I used it for osteoblasts differentiation, Arizarin staining.It keeps and promotes osteoblasts mineralization very well.
    Verified Customer | Posted 05/27/2021
    Recombinant Human/Mouse/Rat BMP-2 Protein Stem/Immune cell maintenance or differentiation  355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Reason for Rating: I used BMP2 to activate BMP reporter and it worked very well.
    Verified Customer | Posted 09/15/2020
    Recombinant Human/Mouse/Rat BMP-2 Protein In vitro bioactivity in cell culture 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: CellProlif
    Reason for Rating: Another great product made by R&D Systems. Always produces consistent and reliable results. Great as a control.
    Verified Customer | Posted 10/09/2019
    Recombinant Human/Mouse/Rat BMP-2 Protein Cell Proliferation 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Immunofluorescence for pSMAD 1,5,8 following BMP treatment
    Verified Customer | Posted 10/26/2018
    Recombinant Human/Mouse/Rat BMP-2 Protein Other 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Cody Saylor
    Application: In vitro bioactivity in cell culture
    Reason for Rating: Performed as expected to force differentiation of C2C12's to Osteoblasts
    Verified Customer | Posted 09/07/2017
  • Name: Abby Sukarto
    Verified Customer | Posted 05/03/2017
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Javier Aragón
    Application: BMP-2 Delivery System
    Verified Customer | Posted 01/05/2017
    Recombinant Human/Mouse/Rat BMP-2 Protein Other 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Reason for Rating: Materials performed as expected.
    Verified Customer | Posted 05/13/2016
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Frank Grosso
    Application: Binding assay/Protein-protein interaction
    Reason for Rating: This protein performed exactly as expected.
    Verified Customer | Posted 01/15/2016
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Sample Tested: C2C12 mouse myoblast cell line
    Verified Customer | Posted 10/26/2015
    ALP assay measuring osteogenic induction of C2C12 cells treated with BMP-2.
    Recombinant Human/Mouse/Rat BMP-2 Protein 355-BM

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FAQs for Recombinant Human/Mouse/Rat BMP-2 Protein

  • What is the half-life of a recombinant or natural protein in culture at 37 °C?

    R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

  • What is the difference between the two Recombinant Human/Mouse/Rat BMP-2 Proteins (Catalog # 355-BM and 355-BEC)?

    Catalog # 355-BM is produced in a mammalian system using CHO cells and exhibits better activity in our routine QC bioassay, while Catalog # 355-BEC is produced in an E. coli expression system and is less expensive. Bioactivity of both Human/Mouse/Rat BMP-2 Proteins is measured in an alkaline phosphatase assay using by ATDC5 mouse chondrogenic cells. For 355-BM, the ED50 for this effect is 40-200 ng/mL, while for 355-BEC, the ED50 range is 80-480 ng/mL. We recommend reviewing our list of publications under the Citations tab for both 355-BM and 355-BEC to determine which of the two proteins is most suitable for your experiment.

  • What is the sequence homology between human, mouse and rat BMP-2

    Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2.

  • Does Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) have a histidine tag?

    Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) does not contain a histidine tag. The sequence of this protein contains Gln283-Arg396 aa based on Accession # P12643.

Product Documents for Recombinant Human/Mouse/Rat BMP-2 Protein

Certificate of Analysis

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