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Y-27632 dihydrochloride

Rho-kinase Inhibitors Small Molecule
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Key Product Details

Description: Selective ROCK inhibitor; inhibits ROCK1 (p160 ROCK) and ROCK2; stem cell freezing (cryopreservation) media component
Alternative Names: y27632, Y-27632 2HCl, ROCK inhibitor, ROCK1 inhibitor
Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride
Purity: ≥98% (HPLC)
Molecular Weight: 320.26

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Citations (244)

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Customer Reviews (11)

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  • ROCK Inhibitor
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Organoids
    To generate human intestinal organoids
  • test
    Name: Veselina Petrova
    Species: Human
    Assay Type: In Vitro
    I used this product on freshly split and plate human iPSC cells to help cell survival and adherence. I used the product at 10 mM concentration which I diluted in 1:1000 in culture media and left the cells at 37C, 5% CO2 for 24 hours before replacing with fresh culture media.
  • For hPSC culture
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Human PSC
    For hPSC culture, final concentration 10mM. Reconstitute 10 mg of Y27632 in 3.0792 ml of PBS. Make 100µl aliquots and keep them at −20 °C for up to 6 months.
    Y-27632 dihydrochloride Serum 1254
  • Worked great and at a great price
    Name: Tres Davis
    Species: Mouse
    Assay Type: Ex Vivo
    HPLC
  • Neural Induction Conditions
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: H9
    ROCK inhibitor Y-27632 (10 mM)
    Y-27632 dihydrochloride Serum 1254
  • Great for passaging stem cells.
    Name: Samiha Shaikh
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC
    Great for passaging huiPSCs with dissociation into single cells.
    Y-27632 dihydrochloride Serum 1254
  • Great for single iPS cell survival
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC
    Used during single cell passage for CRISPR clone isolation, works well and no difference found between multiple batches. Cells show characteristic 'spiky' morphology upon addition to the medium.
    I use at 1:1000 and store at -20C. I remove the compound from medium 24 hours after plating.
    Y-27632 dihydrochloride Serum 1254
  • For iPSC culture
    Name: Pinyuan Tian
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC
    For iPSC culture, final concentration 10mM. Reconstitute 10 mg of Y27632 in 3.0792 ml of PBS.
    Make 100µl aliquots and keep them at −20 °C for up to 6 months.
    Y-27632 dihydrochloride Serum 1254
  • ROCK Inhibitor to improve cartilage integrity
    Name: Anonymous
    Species: Other
    Assay Type: In Vitro
    Cell Line/Tissue: chondrocyte
    I dissolved in DMSO
    Y-27632 dihydrochloride Serum 1254
  • I prepare 20mg of the product in 5.88mL H2O and I use 1uL/mL of the stock in my cell culture after the cell splitting
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: human foreskin fibroblasts-derived-IPS
    I used it in two human IPS cell lines, 5uM concentration the first 24 h after the splitting. The use of this reagent improves substantially the cell survival.
    Keep the stock frozen at -20°C. I use it freshly added to the medium when splitting the cells.
    Y-27632 dihydrochloride Serum 1254
  • ROCK Inhibitor to improve cell survival
    Name: Hudson DE OLIVEIRA BEZERRA
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: H1 (W01)
    My team uses this product to improve human embryonic stem cells survival in single cell passage methods in siRNA and CRISPR experiments. In general, we use the concentration of 10 uM. The products work very well, better than others available in the market.
    Y-27632 dihydrochloride Serum 1254

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