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Y-27632 dihydrochloride

Cat.No. 1254 - Y-27632 dihydrochloride | C14H21N3O.2HCl | CAS No. 129830-38-2

Key Product Details

Description: Selective ROCK inhibitor; inhibits ROCK1 (p160 ROCK) and ROCK2; stem cell freezing (cryopreservation) media component
Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride
Purity: ≥98% (HPLC)

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Citations (244)

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Customer Reviews

5 out of 5
11 customer ratings
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Showing 1-5 of 11 reviews
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  • ROCK Inhibitor
    Name: Anonymous | 7th Sep 2020
    Species: Human
    Assay Type: In Vitro
    To generate human intestinal organoids
  • test
    Name: Veselina Petrova | 28th Aug 2020
    Species: Human
    Assay Type: In Vitro
    I used this product on freshly split and plate human iPSC cells to help cell survival and adherence. I used the product at 10 mM concentration which I diluted in 1:1000 in culture media and left the cells at 37C, 5% CO2 for 24 hours before replacing with fresh culture media.
  • For hPSC culture
    Name: Anonymous | 30th Jun 2020
    Species: Human
    Assay Type: In Vitro
    For hPSC culture, final concentration 10mM. Reconstitute 10 mg of Y27632 in 3.0792 ml of PBS. Make 100µl aliquots and keep them at −20 °C for up to 6 months.
  • Worked great and at a great price
    Name: Tres Davis | 8th May 2020
    Species: Mouse
    Assay Type: Ex Vivo
    HPLC
  • Neural Induction Conditions
    Name: Anonymous | 22nd Mar 2020
    Species: Human
    Assay Type: In Vitro
    ROCK inhibitor Y-27632 (10 mM)
  • Great for passaging stem cells.
    Name: Samiha Shaikh | 31st Jan 2020
    Species: Human
    Assay Type: In Vitro
    Great for passaging huiPSCs with dissociation into single cells.
  • Great for single iPS cell survival
    Name: Anonymous | 13th Sep 2019
    Species: Human
    Assay Type: In Vitro
    Used during single cell passage for CRISPR clone isolation, works well and no difference found between multiple batches. Cells show characteristic 'spiky' morphology upon addition to the medium.
  • For iPSC culture
    Name: Pinyuan Tian | 7th Aug 2018
    Species: Human
    Assay Type: In Vitro
    For iPSC culture, final concentration 10mM. Reconstitute 10 mg of Y27632 in 3.0792 ml of PBS.
  • ROCK Inhibitor to improve cartilage integrity
    Name: Anonymous | 26th Mar 2018
    Species: Other
    Assay Type: In Vitro
    I dissolved in DMSO
  • I prepare 20mg of the product in 5.88mL H2O and I use 1uL/mL of the stock in my cell culture after the cell splitting
    Name: Anonymous | 1st Feb 2018
    Species: Human
    Assay Type: In Vitro
    I used it in two human IPS cell lines, 5uM concentration the first 24 h after the splitting. The use of this reagent improves substantially the cell survival.
  • ROCK Inhibitor to improve cell survival
    Name: Hudson DE OLIVEIRA BEZERRA | 5th Jan 2018
    Species: Human
    Assay Type: In Vitro
    My team uses this product to improve human embryonic stem cells survival in single cell passage methods in siRNA and CRISPR experiments. In general, we use the concentration of 10 uM. The products work very well, better than others available in the market.

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