Forskolin

Name: Forskolin
Brand: Tocris Bioscience
SKU: 1099
Rating: 5 (6 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1099

(6)

Citations (936)

COA / SDS

Adenylyl cyclase activator

Key Product Details

Description:	Adenylyl cyclase activator
Chemical Name:	(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-5-(Acetyloxy)-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1H-naphtho[2,1-b]pyran-1-one
Purity:	≥98% (HPLC)
Molecular Weight:	410.51

View Full Technical Details for Forskolin

Technical Data
Citations / Reviews (6)

Biological Activity for Forskolin

Forskolin, or coleonol, is a cell-permeable, potent, reversible and rapid activator of adenylyl cyclase, an enzyme that converts ATP to cAMP and pyrophosphate (EC₅₀ = 0.5 μM). In combination with other compounds, forskolin enables chemical reprogramming of mouse embryonic fibroblasts to pluripotent stem cells, without genetic factors (protocol available). In combination with IBMX (Cat. No. 2845), forskolin induces neural differentiation of mesenchymal stem cells by downregulating neuron restrictive silencer factor. In a rat model of Huntington's disease-like neurodegeneration, forskolin also exhibits neuroprotective effects. Hypotensive and vasodilatory agent.

Water-soluble analog, NKH 477 (Cat. No. 1603), and Forskolin synthesized to Ancillary Material Grade also available.

For more information about how Forskolin may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Reprogramming Mouse Embryonic Fibroblasts (MEFs) into Skeletal Muscle Progenitors, Highly Efficient Generation of Chemically Induced Pluripotent Stem Cells (CiPSCs) from Mouse Embryonic Fibroblasts (MEFs).

Compound Libraries for Forskolin

Forskolin is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Scientific Data Examples for Forskolin

	Application of Forskolin in Culture and Characterization of Adult Stem Cell-derived Liver Organoids. Adult stem cell-derived liver organoids were cultured using Forskolin, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), N-Acetylcysteine (Catalog # 7874), along with other reagents used in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes HEPES (Catalog # 3173), Nicotinamide (Catalog # 4106), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), along with other reagents. Differentiated human liver organoids were stained using a (A) Human Serum Albumin Antibody (Catalog # MAB1455; red) and a (B) Human HNF-3 beta /FoxA2 Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).

Technical Data for Forskolin

M. Wt	410.51
Formula	C₂₂H₃₄O₇
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	66575-29-9
PubChem ID	47936
InChi Key	OHCQJHSOBUTRHG-KGGHGJDLSA-N
Smiles	C=C[C@](C3)(C)O[C@]2(C)[C@@H](OC(C)=O)[C@@H](O)[C@@]1([H])C(C)(C)CC[C@H](O)[C@@](C)1[C@](O)2C3=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Forskolin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	4.11	10
	DMSO	10.26	25

Preparing Stock Solutions for Forskolin

The following data is based on the product molecular weight 410.51.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	9.74 mL	48.72 mL	97.44 mL
1.25 mM	1.95 mL	9.74 mL	19.49 mL
2.5 mM	0.97 mL	4.87 mL	9.74 mL
12.5 mM	0.19 mL	0.97 mL	1.95 mL

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References for Forskolin

References are publications that support the biological activity of the product.

Sato SnapShot: Growing Organoids from Stem Cells. Cell 2015 PMID: 26091044
Robbins Forskolin carbamates: binding and activation studies with type I adenylyl cyclase. J.Med.Chem. 1996 PMID: 8709105
Hou Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science 2013 PMID: 23868920
Kim cAMP induces neuronal differentiation of mesenchymal stem cells via activation of extracellular signal-regulated kinase/MAPK. Neuroreport 2005 PMID: 16056139
Seamon Structure-activity relationships for activation of adenylate cyclase by the diterpene forskolin and its derivatives. J.Med.Chem. 1983 PMID: 6681845
Laurenza Forskolin: a specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action? TiPS 1989 PMID: 2692256
Awad Interactions of forskolin and adenylate cyclase. J.Biol.Chem. 1983 PMID: 6681815