NKH 477

Name: NKH 477
Brand: Tocris Bioscience
SKU: 1603
Price: 258 USD

Tocris Bioscience a Bio-Techne Brand

Catalog # 1603

Citations (7)

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Key Product Details

Description:	Water-soluble adenylyl cyclase activator
Alternative Names:	Colforsin dapropate hydrochloride, 6-(3-dimethylaminopropionyl)forskolin hydrochloride
Chemical Name:	N,N-Dimethyl-(3R,4aR,5S,6aS,10S,10aR,10bS)-5-(acetyloxy)-3-ethenyldodecahydro-10,10b-dihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-1H-naphtho[2,1-b]pyran-6-yl ester β-alanine hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	546.1

View Full Technical Details for NKH 477

Biological Activity for NKH 477

NKH 477 is a water-soluble analog of forskolin (Cat. No. 1099) that is a potent activator of adenylyl cyclase; shows some selectivity for cardiac (type V) adenylyl cyclase. Stimulates bronchodilation (EC₅₀ = 32.6 nM) and is an orally active potent positive chronotrope and hypotensive agent in vivo.

Technical Data for NKH 477

M. Wt	546.1
Formula	C₂₇H₄₃NO₈.HCl
Storage	Desiccate at +4°C
Purity	≥98% (HPLC)
CAS Number	138605-00-2
PubChem ID	444028
InChi Key	VIRRLEDAYYYTOD-YHEOSNBFSA-N
Smiles	O=C(CCN(C)C)O[C@H]2[C@@]1([H])C(C)(C)CC[C@H](O)[C@@](C)1[C@]3(O)[C@@](O[C@](C=C)(C)CC3=O)(C)[C@H]2OC(C)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NKH 477

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	21.84	40

Preparing Stock Solutions for NKH 477

The following data is based on the product molecular weight 546.1.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.4 mM	4.58 mL	22.89 mL	45.78 mL
2 mM	0.92 mL	4.58 mL	9.16 mL
4 mM	0.46 mL	2.29 mL	4.58 mL
20 mM	0.09 mL	0.46 mL	0.92 mL

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References for NKH 477

References are publications that support the biological activity of the product.

Satake Relaxant effects of NKH477, a new water-soluble forskolin derivative, on guinea-pig tracheal smooth muscle: the role of Ca²⁺-activated K⁺ channels. Br.J.Pharmacol. 1998 PMID: 9517396
Toya Forskolin derivatives with increased selectivity for cardiac adenylyl cyclase. J.Mol.Cell.Cardiol. 1998 PMID: 9500868
Hosono Cardiovascular and adenylate cyclase stimulant properties of NKH477, a novel water-soluble forskolin derivative. J.Cardiovasc.Pharmacol. 1992 PMID: 1380607

Citations for NKH 477 (7)

Showing 1 - 7 of 7 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for NKH 477 include:

Frieling (2017) Matrix metalloproteinase processing of PTHrP yields a selective regulator of osteogenesis, PTHrP1-17. Oncogene. 36(31):4498
PMID: 28368420
Park et al. (2016) Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS. Nat Immunol. 17:914
PMID: 27270401
Muschter et al. (2015) Sympathetic Neurotransmitters Modulate Osteoclastogenesis and Osteoclast Activity in the Context of Collagen-Induced Arthritis. PLoS One. 10:e0139726
PMID: 26431344
Brosnahan et al. (2013) NE potentiates proinflammatory responses of human vaginal epithelial cells. Cell Mol Neurobiol. 259:42583
PMID: 23571017
Lee et al. (2012) The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca²⁺ and cAMP. Nature. 492:123
PMID: 23143333
Zhong et al. (2012) Phosphodiesterase 4 inhibition impairs cocaine-induced inhibitory synaptic plasticity and conditioned place preference. Neuropsychopharmacology. 37:2377
PMID: 22713909
Xia et al. (2011) Inhibition of morphine-induced cAMP overshoot: a cell-based assay model in a high-throughput format. PLoS One. 31:901
PMID: 21598037

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