Human Serum Albumin Antibody

Name: Human Serum Albumin Antibody
Brand: R&D Systems
SKU: MAB1455
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1455

Citations (31)

(2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Human Serum Albumin
Applications:	CyTOF-ready, Dual RNAscope ISH-IHC, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 188835
Specificity:	Detects human Serum Albumin in direct ELISAs and Western blots. Does not detect mouse Serum Albumin in direct ELISAs and Western blots.

View Full Technical Details for Human Serum Albumin Antibody

Applications for Human Serum Albumin Antibody

	Recommended Concentration	Sample
Western Blot	0.2-1 µg/mL	See below
Simple Western	1-10 µg/mL	See below
Immunohistochemistry	0.1-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Dual RNAscope ISH-IHC	0.05-25 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Serum Albumin Antibody

	Western Blot Detection of Human Albumin by Western Blot. Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry Albumin in Hepatocytes Derived from Human Embryonic Stem Cells. Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry Albumin in Human Liver. Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Intracellular Staining by Flow Cytometry Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
	Simple Western Detection of Human Albumin by Simple Western^TM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Simple Western Detection of Human Albumin by Simple Western^TM. Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Dual RNAscope ISH-IHC Compatible Serum Albumin in Human Liver Using Dual RNAscope^®ISH and IHC. Serum Albumin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human liver probed with ACD RNAScope^®Probe (Catalog # 600941) and stained using ACD RNAscope^®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.05 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Albumin

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Item	Value
Entrez Gene IDs	213 (Human); 11657 (Mouse); 24186 (Rat)
Alternate Names	ALB, Albumin, DKFZp779N1935, PRO0883, PRO0903, PRO1341, cell growth inhibiting protein 42, growth-inhibiting protein 20, serum albumin

Citations for Human Serum Albumin Antibody (31)

Showing 1 - 10 of 31 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Serum Albumin Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

MAS de Toledo et al. (2022), CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout Stem Cell Research, 60(0):102732.
PMID: 35279545
Species: Human
Sample Types: Whole Cells
Applications: ICC/IF

S Ghafoory et al. (2022), Oxygen Gradient Induced in Microfluidic Chips Can Be Used as a Model for Liver Zonation Cells, 11(23).
PMID: 36496994
Species: Human
Sample Types: Extracellular Vesicles
Applications: Western Blot

M Ramirez-Ga et al. (2021), Potential of miRNAs in urinary extracellular vesicles for management of active surveillance in prostate cancer patients British Journal of Cancer, 0(0).
PMID: 34811506
Species: Human
Sample Types: Whole Cells
Applications: ICC

ES Famulari et al. (2020), Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice Sci Rep, 10(1):887.
PMID: 31965023
Species: Human
Sample Types: Whole Tissue
Applications: IHC

N Mori et al. (2020), Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue Sci Rep, 10(1):5646.
PMID: 32286353
Species: Human
Sample Types: Whole Cells
Applications: ICC

G Sorrentino et al. (2020), Mechano-modulatory synthetic niches for liver organoid derivation Nat Commun, 11(1):3416.
PMID: 32651372
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Liu et al. (2020), Repair of acute liver damage with immune evasive hESC derived hepato-blasts Stem Cell Res, 49(0):102010.
PMID: 33011360
Species: Mouse
Sample Types: Protein
Applications: Western Blot

M Golizeh et al. (2020), Fecal host biomarkers predicting severity of Clostridioides difficile infection JCI Insight, 0(0).
PMID: 33232301
Species: Human
Sample Types: Organoid
Applications: IHC

F Michielin et al. (2020), The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs Cell Rep, 33(9):108453.
PMID: 33264615
Species: Human
Sample Types: Whole Cells
Applications: ICC

PA Subramani et al. (2020), Plasmodium vivax liver stage assay platforms using Indian clinical isolates Malar. J., 19(1):214.
PMID: 32571333
Species: Human
Sample Types: Whole Cells
Applications: ICC

H Okada et al. (2019), Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia Sci Rep, 9(1):4695.
PMID: 30886174
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

C Gu et al. (2019), The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium J. Cell. Mol. Med., 0(0).
PMID: 30950200
Species: Human
Sample Types: Peritoneal Dialysate
Applications: ELISA Detection

AC Brook et al. (2019), Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis Sci Rep, 9(1):10136.
PMID: 31300703
Species: Human
Sample Types: Whole Cells
Applications: ICC

F Tonon et al. (2019), In vitro metabolic zonation through oxygen gradient on a chip Sci Rep, 9(1):13557.
PMID: 31537830
Species: Rat
Sample Types: Whole Cells
Applications: ICC

A Fernández- et al. (2018), 4 in 1: Antibody-free protocol for isolating the main hepatic cells from healthy and cirrhotic single rat livers J. Cell. Mol. Med., 0(0).
PMID: 30417530
Species: Human
Sample Types: Whole Cells
Applications: ICC

A Skrzypczyk et al. (2018), Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes Stem Cells Int, 2018(0):5692840.
PMID: 30210551
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MB Herrera Sa et al. (2017), Extracellular vesicles from human liver stem cells restore argininosuccinate synthase deficiency Stem Cell Res Ther, 8(1):176.
PMID: 28750687
Species: Human
Sample Types: Whole Cells
Applications: ICC

CI Real et al. (2017), Nucleic acid-based polymers effective against hepatitis B Virus infection in patients don't harbor immunostimulatory properties in primary isolated liver cells Sci Rep, 7(0):43838.
PMID: 28272460
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-Fr

(2016), Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model Stem Cells Dev, 0(0).
PMID: 27676103
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

(2016), A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood Nat Commun, 7(0):13363.
PMID: 27874859
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Elmes M et al. (2015), Fatty acid-binding proteins (FABPs) are intracellular carriers for Delta9-tetrahydrocannabinol (THC) and cannabidiol (CBD). J Biol Chem, 290(14):8711-21.
PMID: 25666611
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Park H et al. (2015), Differences in the Epigenetic Regulation of Cytochrome P450 Genes between Human Embryonic Stem Cell-Derived Hepatocytes and Primary Hepatocytes. PLoS ONE, 10(7):e0132992.
PMID: 26177506
Yamamizu K et al. (2013), Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports, 1(6):545-59.
PMID: 24371809
Species: Human
Sample Types: Whole Cells
Applications: ICC

Hannan et al. (2013), Production of hepatocyte-like cells from human pluripotent stem cells. Nat Protoc, 8(2):430-7.
PMID: 23370178
Species: Human
Sample Types: Whole Cells
Applications: ICC

Byun K et al. (2012), Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease. PLoS ONE, 7(5):e37917.
PMID: 22662249
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Hoang S et al. (2009), Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia. J. Cereb. Blood Flow Metab., 29(2):385-97.
PMID: 18985053
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Yang L et al. (2008), Fate-mapping evidence that hepatic stellate cells are epithelial progenitors in adult mouse livers. Stem Cells, 26(8):2104-13.
PMID: 18511600
Species: Human
Sample Types: Whole Cells
Applications: ICC

Ilancheran S et al. (2007), Stem cells derived from human fetal membranes display multilineage differentiation potential. Biol. Reprod., 77(3):577-88.
PMID: 17494917
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Zeisberg M et al. (2007), Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition. J. Biol. Chem., 282(32):23337-47.
PMID: 17562716
Species: Human
Sample Types: Whole Cells
Applications: ICC

Baharvand H et al. (2006), Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro. Int. J. Dev. Biol., 50(7):645-52.
PMID: 16892178
Species: Human
Sample Types: Whole Cells
Applications: ICC

Herrera MB et al. (2006), Isolation and characterization of a stem cell population from adult human liver. Stem Cells, 24(12):2840-50.
PMID: 16945998

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Customer Reviews for Human Serum Albumin Antibody (2)

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Human Serum Albumin Antibody

Name: Anonymous

Application: IHC

Sample Tested: Tonsil tissue

Species: Human

Verified Customer | Posted 08/20/2021
Human/Mouse/Rat Serum Albumin Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Human recombinant antibody

Species: Human

Verified Customer | Posted 10/25/2017

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Product Documents for Human Serum Albumin Antibody

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Human Serum Albumin Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human Serum Albumin Antibody

Scientific Data Examples for Human Serum Albumin Antibody

Western Blot

Immunocytochemistry

Immunohistochemistry

Intracellular Staining by Flow Cytometry

Simple Western

Simple Western

Dual RNAscope ISH-IHC Compatible

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Albumin

Citations for Human Serum Albumin Antibody (31)

Customer Reviews for Human Serum Albumin Antibody (2)

Customer Images

Human Serum Albumin Antibody

Human/Mouse/Rat Serum Albumin Antibody

Product Documents for Human Serum Albumin Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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