Human Serum Albumin APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1455A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 188835
Product Specifications
Immunogen
Human Serum Albumin
Specificity
Detects human Serum Albumin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Serum Albumin APC-conjugated Antibody
Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin APC-conjugated Monoclonal Antibody (Catalog # IC1455A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Detection of Albumin in THP-1 Human Cell Line by Flow Cytometry
THP-1 human monocytic leukemia cell line (negative control) was stained with Mouse Anti-Human Serum Albumin APC-conjugated Monoclonal Antibody (Catalog # IC1455A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human Serum Albumin APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.01-0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line and THP-1 human monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: HepG2 human hepatocellular carcinoma cell line and THP-1 human monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra ViewerFormulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Albumin
Additional Albumin Products
Product Documents for Human Serum Albumin APC-conjugated Antibody
Product Specific Notices for Human Serum Albumin APC-conjugated Antibody
For research use only
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