N-Acetylcysteine
Tocris Bioscience, part of Bio-Techne | Catalog # 7874
Glutathione (GSH) precursor; maintains stem cell function in culture.
Key Product Details
Description
Glutathione (GSH) precursor; maintains stem cell function in culture.
Product Description
N-Acetylcysteine is a cell-permeable antioxidant and a precursor of reduced glutathione (GSH) (Cat. No. 5219), with anti-inflammatory, mucolytic, and antiviral activities. N-Acetylcysteine can be used in organoid culture media for non-small cell lung and colorectal cancer organoids, expansion media for mouse/human liver and pancreas 3D organoids and LWRN media for colonic 3D organoids from human biopsies. It is a component of S7 medium in the optimized protocol for generation of functional stem cell-derived islets (SC-islets). N-Acetylcysteine can be orally administered.Scientific Data Images for N-Acetylcysteine
Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Lung Organoids.
Adult stem cells isolated from human lung biopsy tissue were embedded and then cultured for 20-60 days in lung organoid expansion medium, which includes N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), SB 202190 (Catalog # 1264), Nicotinamide (Catalog # 4106), along with other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various lung cell types. Lung organoids were stained with (A) anti-Cytokeratin 5 (KRT5) (green) and with anti-p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (B) Anti-Podoplanin (PDPN) (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and anti- p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (C, D) Anti-MUC5AC (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and anti-SOX2 (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).
Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Liver Organoids.
Adult stem cell-derived liver organoids were cultured using N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), Forskolin (Catalog # 1099), along with other reagents used in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes N-Acetylcysteine, HEPES (Catalog # 3173), Nicotinamide (Catalog # 4106), A 83-01 (Catalog # 2939), along with other reagents. Differentiated human liver organoids were stained using a (A) Human Serum Albumin Antibody (Catalog # MAB1455; red) and a (B) Human HNF-3beta /FoxA2 Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).
Application of N-Acetylcysteine in Culture and Characterization of iPSC-derived Human Intestinal Organoids.
iPSC-derived human intestinal organoids were cultured using N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Human/Mouse/Rat Vimentin Antibody (Catalog # MAB2105; green) and a Human/Mouse Desmin Antibody (Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Human/Mouse E-Cadherin Antibody (Catalog # AF748; green), a Human MUC2 Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Catalog # 5748; blue).Product Specifications
Molecular Weight
163.20
Formula
C5H9NO3S
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(2R)-2-Acetamido-3-sulfanylpropanoic acid
CAS Number
616-91-1
PubChem ID
12035
InChI Key
PWKSKIMOESPYIA-BYPYZUCNSA-N
SMILES
[C@@H](NC(C)=O)(C(O)=O)CS
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 8.16 | 50 | |
| DMSO | 8.16 | 50 |
Preparing Stock Solutions
for N-Acetylcysteine
The following data is based on the product molecular weight 163.20
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 12.25 mL | 61.27 mL | 122.55 mL |
| 2.5 mM | 2.45 mL | 12.25 mL | 24.51 mL |
| 5 mM | 1.23 mL | 6.13 mL | 12.25 mL |
| 25 mM | 0.25 mL | 1.23 mL | 2.45 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Geiler N-acetyl-L-cysteine (NAC) inhibits virus replication and expression of pro-inflammatory molecules in A549 cells infected with highly pathogenic H5N1 influenza A virus. Biochem.Pharmacol. 2009 PMID: 19732754
- Moiseeva Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration. Nature 2023 PMID: 36544018
- Broutier Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat.Protoc. 2016 PMID: 27560176
- Balboa Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells. Nat.Biotechnol. 2022 PMID: 35241836
- Cattaneo Tumor organoid-T-cell coculture systems. Nat.Protoc. 2019 PMID: 31853056
Product Specific Notices for N-Acetylcysteine
For research use only
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