HEPES

Name: HEPES
Brand: Tocris Bioscience
SKU: 3173
Rating: 5 (3 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 3173

Citations (1)

(3)

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Key Product Details

Description:	Multipurpose HEPES buffer; used in cell culture
Chemical Name:	4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Purity:	≥99.5%
Molecular Weight:	238.3

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Biological Activity for HEPES

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

For more information about how HEPES may be used, see our protocol:

3D Culture of Lung Alveolar Cells

Technical Data for HEPES

M. Wt	238.3
Formula	C₈H₁₈N₂O₄S
Storage	Store at RT
Purity	≥99.5%
CAS Number	7365-45-9
PubChem ID	23830
InChi Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Smiles	OCCN1CCN(CCS(=O)(O)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for HEPES

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	238.3	1000

Preparing Stock Solutions for HEPES

The following data is based on the product molecular weight 238.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
10 mM	0.42 mL	2.10 mL	4.20 mL
50 mM	0.08 mL	0.42 mL	0.84 mL
100 mM	0.04 mL	0.21 mL	0.42 mL
500 mM	0.01 mL	0.04 mL	0.08 mL

Molarity Calculator

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Reconstitution Calculator

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References for HEPES

References are publications that support the biological activity of the product.

Medzon and Gedies Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol.
Good Hydrogen ion buffers for biological research. Biochemistry 1966 PMID: 5942950

Citations for HEPES (1)

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Citations are publications that use Bio-Techne products. Selected citations for HEPES include:

Lerner et al. (2010) Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists. J Neurosci. 30:2160
PMID: 20147543

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Customer Reviews for HEPES (3)

4.3 out of 5

3 customer ratings

5 stars

33%

4 stars

67%

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HEPES (10 mM) maintains pH in the experimental buffer for Ca2+ imaging.

Name: Anonymous

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: HEK293

HEPES (10 mM) is used to maintain pH 7.4 in the experimental buffer for Ca2+ imaging, consisting of 137 mM NaCl, 5.4 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 10 mM glucose.
Good buffer

Name: Anonymous

Species: Mouse

Assay Type: Ex Vivo

Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.
HEPES to buffer pH

Name: Anonymous

Species: Mouse

Assay Type: Ex Vivo

Cell Line/Tissue: Brain slice

HEPES used in solutions, good pH buffer.

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Product Documents for HEPES

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.