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CHIR 99021

GSK-3 Inhibitors Small Molecule
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4423/50
4423/10

Key Product Details

Description: Highly selective GSK-3 inhibitor; acts as Wnt activator
Alternative Names: CHIR99021, CT99021
Chemical Name: 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
Purity: ≥98% (HPLC)
Molecular Weight: 465.34

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Customer Reviews (13)

4.9 out of 5
13 customer ratings
5 stars
92%
4 stars
8%
3 stars
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  • Differentiation to cartilage
    Name: Julieta O'Flaherty
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: iPSCs
    I used at 3uM to generate cartilage from iPSCs, worked really well. Keep at -80C for long term storage
    CHIR 99021 Serum 4423
  • Kidney organoids
    Name: Kirsty Rooney
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: MAN13 hESCs
    I use 8uM of CHIR to differentiate hESCs to intermediate mesoderm and then to kidney organoids
    CHIR 99021 Serum 4423
  • Differentiation of human pluripotent stem cells
    Name: Paul Humphreys
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: hPSCs
    Directed differentiation of human pluripotent stem cells (hPSCs) towards mesoderm lineages. Used at concentration of 3uM. Very effective in induction of mesoderm markers.
    Perform titration against your cell line to optimise correct concentration.
    CHIR 99021 Serum 4423
  • iPSC differentiation
    Name: Stephanie Hsiao
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC
    Differentiation of human iPSCs into hematopoietic progenitor cells.
    iPSC culture medium supplemented with 4 uM of CHIR99021.
    CHIR 99021 Serum 4423
  • It worked good
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC
    Reprogramming media comprises of 3 μM CHIR99021 and 0.5 μM A-83-01.
    The cells were then maintained in RM 15 days with fresh media every other day
    CHIR 99021 Serum 4423
  • For maintenance of stem cells pluripotency
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Neural Stem Cells
    For directed differentiation to neural, cells are treated with GSK-3 inhibitor, CHIR99021 to induce canonical WNT signaling. This product gave me reproducible results.
    It's better to aliquot and keep it at -80°C for long term storage.
    CHIR 99021 Serum 4423
  • Works as intended.
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: Sprague-Dawley rats primary neuronal
    10 μM for 2 h
    CHIR 99021 Serum 4423
  • Differentiation of human dopaminergic neurons from stem cells
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Stem Cells
    Used this product routinely to differentiate dopaminergic neurons from human stem cells.
    CHIR 99021 Serum 4423
  • The hiPSCs were maintained with Essential 8 Flex Medium with CHIR 99021
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: ipsc
    1.4 μM
    CHIR 99021 Serum 4423
  • All iPSC lines and R1 mouse ES cells were maintained on feeders in KO-DMEM (Invitrogen) with 3 mM CHIR99021, and 1 mM PD0325901.
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: ipsc
    All iPSC lines and R1 mouse ES cells were maintained on feeders in KO-DMEM (Invitrogen) with 3 mM CHIR99021, and 1 mM PD0325901.
    CHIR 99021 Serum 4423
  • works great for Wnt activation
    Name: avinash waghray
    Species: Human
    Assay Type: In Vitro
    In Tissue culture for growing basal cells
  • Check the maintenance of naive pluripotency in 2i
    Name: Megha PB
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: E14TG2a
    Culture mouse ES cells in 2i media and without 2i to assess differentiation. Only the cells in 2i are pluripotent while mES cells cultured in -2i for 48 hours exit naive pluripotency.
    CHIR 99021 Serum 4423
  • reliable product
    Name: Pinyuan Tian
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: pluripotent stem cells
    For directed differentiation of pluripotent stem cells to kidney organoids, cells are treated with GSK-3 inhibitor, CHIR99021 to induce canonical WNT signaling. CHIR99021 concentration and differentiation time period are the key factors for this protocol. I found this product gave me reproducible results.
    Although the datasheet says store at -20°C, it's better to aliquot and keep it at -80°C for long term storage.
    CHIR 99021 Serum 4423

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