CHIR 99021

Citations are publications that use Bio-Techne products. Selected citations for CHIR 99021 include:

• Xiong et al. (2021) Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function cell stem Cell. 28:112
  PMID: 32966778
• Kim et al. (2021) Biphasic activation of WNT signaling facilitates the derivation of midbrain dopamine neurons from hESCs for translational use. Cell Stem Cell. 28:343
  PMID: 33545081
• Gao et al. (2019) Establishment of porcine and human expanded potential stem cells. Nat Cell Biol. 21:687
  PMID: 31160711
• Schneider et al. (2019) User-Friendly and Parallelized Generation of Human Induced Pluripotent Stem Cell-Derived Microtissues in a Centrifugal Heart-on-a-Chip. Tissue Eng Part A. 25:786
  PMID: 30968738
• Regier et al. (2019) User-defined morphogen patterning for directing human cell fate stratification. Sci Rep. 9:6433
  PMID: 31015521
• Hackland et al. (2019) FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision. Stem Cell Reports. 12:920
  PMID: 31091435
• Genga et al. (2019) Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Cell Rep. 27:708
  PMID: 30995470
• Park et al. (2019) Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction. Nat Commun. 10:3123
  PMID: 31311935
• Yap et al. (2019) In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors. Cell Rep. 26:3231
  PMID: 30893597
• Heuberger et al. (2019) A C/EBPα-Wnt connection in gut homeostasis and carcinogenesis. Life Sci Alliance. 2:e201800173
  PMID: 30599048
• Ranganathan et al. (2019) Evaluating Shigella flexneri Pathogenesis in the Human Enteroid Model. Infect Immun. 87
  PMID: 30642900
• Varghese et al. (2019) Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth Factor vs. Small Molecule-Based Approaches. Stem Cells Int. 2019:5968236
  PMID: 30805010
• Zhang et al. (2019) Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells. Nat Commun. 10:496
  PMID: 30700702
• Lang et al. (2019) Single-Cell Sequencing of iPSC-DA Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes. Cell Stem Cell. 24:93
  PMID: 30503143
• Yin et al. (2019) Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways. Stem Cell Reports. 12:488
  PMID: 30745031
• Wimmer et al. (2019) Human blood vessel organoids as a model of diabetic vasculopathy. Nature. 565:505
  PMID: 30651639
• Haidar et al. (2019) Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies. Autophagy. 15:1051
  PMID: 30669930
• Vila et al. (2019) Quantification of human neuromuscular function through optogenetics. Theranostics. 9:1232
  PMID: 30867827
• Gentsch et al. (2019) Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals. Nat Commun. 10:4269
  PMID: 31537794
• Canizo et al. (2019) A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos. BMC Dev Biol. 19:13
  PMID: 31272387
• Frith and Tsakiridis (2019) Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate. Curr Protoc Stem Cell Biol. :e81
  PMID: 30688409
• Xu et al. (2019) Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system. Stem Cell Res Ther. 10:193
  PMID: 31248457
• Du et al. (2019) Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg-/- mice. Protein Cell. 10:31
  PMID: 29948854
• Chiu et al. (2019) NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content. Cell Rep. 28:332
  PMID: 31291571
• Garcia et al. (2019) Huntington's Disease Patient-Derived Astrocytes Display Electrophysiological Impairments and Reduced Neuronal Support. Front Neurosci. 13:669
  PMID: 31316341
• Suzuki et al. (2019) Directed differentiation of human induced pluripotent stem cells into mature stratified bladder urothelium. Sci Rep. 9:10506
  PMID: 31324820
• Regent et al. (2019) Automation of human pluripotent stem cell differentiation toward retinal pigment epithelial cells for large-scale productions. Sci Rep. 9:10646
  PMID: 31337830
• Canfield et al. (2019) An isogenic neurovascular unit model comprised of human induced pluripotent stem cell-derived brain microvascular endothelial cells, pericytes, astrocytes, and neurons. Fluids Barriers CNS. 16:25
  PMID: 31387594
• Shafa et al. (2018) Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers. Front Med (Lausanne). 5:69
  PMID: 29600249
• Leung et al. (2018) Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells. Stem Cells. 2018;
  PMID: 29569827
• Tumelty et al. (2018) Identification of direct negative cross-talk between the SLIT2 and bone morphogenetic protein-Gremlin signaling pathways. J Biol Chem. 293:3039
  PMID: 29317497
• McCauley et al. (2018) Derivation of Epithelial-Only Airway Organoids from Human Pluripotent Stem Cells. Curr Protoc Stem Cell Biol. 45:e51
  PMID: 30040246
• Sommer et al. (2018) Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease. Cell Stem Cell. 23:123
  PMID: 29979986
• Tata et al. (2018) Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury. Cell Stem Cell. 22:668
  PMID: 29656943
• Bar-Nur et al. (2018) Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors. Stem Cell Reports. 10:1505
  PMID: 29742392
• Bantounas et al. (2018) Generation of functioning nephrons by implanting human pluripotent stem cell-derived kidney progenitors. Stem Cell Reports. 10:766
  PMID: 29429961
• Zhou et al. (2018) Functional comparison of distinct Brachyury+ states in a renal differentiation assay. Biol Open. 7
  PMID: 29666052
• Levardon et al. (2018) Expansion of Airway Basal Cells and Generation of Polarized Epithelium. Bio Protoc. 8
  PMID: 30009215
• Shekhar et al. (2018) ETV1 activates a rapid conduction transcriptional program in rodent and human cardiomyocytes. Sci Rep. 8:9944
  PMID: 29967479
• Liu et al. (2018) CRISPR-based chromatin remodeling of the endogenous Oct4 or Sox2 locus enables reprogramming to pluripotency. Cell Stem Cell.. 22:252
  PMID: 29358044
• Wang et al. (2018) The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest. 128:3319
  PMID: 29939162
• Sharma et al. (2018) Stage-specific Effects of Bioactive Lipids on Human iPSC Cardiac Differentiation and Cardiomyocyte Proliferation. Sci Rep. 8:6618
  PMID: 29700394
• Schmitt et al. (2018) Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling. Cell Rep. 24:2312
  PMID: 30157426
• Rajamani et al. (2018) Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses. Cell Stem Cell. 22:698
  PMID: 29681516
• Lynch et al. (2018) Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium. Cell Stem Cell. 22:653
  PMID: 29656941
• Pham et al. (2018) Generation of human vascularized brain organoids. Neuroreport. 29:588
  PMID: 29570159
• Yamoah et al. (2018) Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles. Int J Nanomedicine. 13:6073
  PMID: 30323594
• Li et al. (2018) Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation. Protein Cell. 2018;
  PMID: 29948855
• Srikanth et al. (2018) Shared effects of DISC1 disruption and elevated WNT signaling in human cerebral organoids. Transl Psychiatry. 8:77
  PMID: 29643329
• Sampaziotis et al. (2017) Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells. Nat Protoc. 12:814
  PMID: 28333915
• Vermilyea et al. (2017) Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts. Stem Cells Dev. 26:1225
  PMID: 28635509
• McCauley et al. (2017) Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling. Cell Stem Cell. 20:844
  PMID: 28366587
• Lancaster et al. (2017) Guided self-organization and cortical plate formation in human brain organoids. Nat Biotechnol. 35:659
  PMID: 28562594
• Morizane and Bonventre (2017) Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Nat Protoc. 12:195
  PMID: 28005067
• Ong et al. (2017) Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes. Sci Rep. 7:4566
  PMID: 28676704
• Yang et al. (2017) Establishment of mouse expanded potential stem cells. Nature. 550:393
  PMID: 29019987
• Lai et al. (2017) Correction of Hirschsprung-Associated Mutations in Human Induced Pluripotent Stem Cells Via Clustered Regularly Interspaced Short Palindromic Repeats/Cas9, Restores Neural Crest Cell Function. Gastroenterology. 153:139
  PMID: 28342760
• Kumar et al. (2017) Dynamic regulation of Nanog and stem cell-signaling pathways by Hoxa1 during early neuro-ectodermal differentiation of ES cells. Proc Natl Acad Sci U S A. 114:5838
  PMID: 28584089
• Tang and Cheng (2017) Cocktail of chemical compounds robustly promoting cell reprogramming protects liver against acute injury. Protein Cell. 8:273
  PMID: 28190217
• Yang et al. (2017) Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. Cell.. 169:243
  PMID: 28388409
• Xi (2017) In Vivo Human Somitogenesis Guides Somite Development from hPSCs. Cell Rep.. 18(6):1573
  PMID: 28178531
• Lin et al. (2017) Culture in Glucose-Depleted Medium Supplemented with Fatty Acid and 3,3',5-Triiodo-l-Thyronine Facilitates Purification and Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes. Front Endocrinol (Lausanne). 8:253
  PMID: 29067001
• Lee et al. (2017) Anatomically and Functionally Distinct Lung Mesenchymal Populations Marked by Lgr5 and Lgr6. Cell. 170:1149
  PMID: 28886383
• Kotton et al. (2017) Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells. Cell Stem Cell.. 21:472
  PMID: 28965766
• Zhao et al. (2017) Efficient differentiation of TBX18(+)/WT1(+) epicardial-like cells from human pluripotent stem cells using small molecular compounds. Stem Cells Dev. 26:528
  PMID: 27927069
• Christoforou et al. (2017) Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage. Sci Rep. 7:40285
  PMID: 28071742
• Tchieu et al. (2017) A modular platform for differentiation of human PSCs into all major ectodermal lineages. Cell Stem Cell.. 21:399
  PMID: 28886367
• Shao (2017) A pluripotent stem cell-based model for post-implantation human amniotic sac development. Nat Commun. 8(1):208
  PMID: 28785084
• Brown (2017) Live imaging reveals that the first division of differentiating human embryonic stem cells often yields asymmetric fates. Cell Rep. 21(2):301
  PMID: 29020617
• Yang (2017) Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinalstem cell niche. Nat Commun. 8:14766
  PMID: 28303901
• Walsh et al. (2017) Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells. Cell Transplant. 26:1890
  PMID: 29390875
• Chal et al. (2016) Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat.Protoc.. 11:1833
  PMID: 27583644
• Zimmerlin et al. (2016) Tankyrase inhibition promotes a stable human na�ve pluripotent state with improved functionality. Development. 143:4368
  PMID: 27660325
• Takasato et al. (2016) Generation of kidney organoids from human pluripotent stem cells. Nature Protocols. 11:1681
  PMID: 27560173
• Woo et al. (2016) Enhancing a Wnt-Telomere Feedback Loop Restores Intestinal Stem Cell Function in a Human Organotypic Model of Dyskeratosis Congenita Cell Stem Cell. 19:397
  PMID: 27545506
• Long et al. (2016) Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Biotechnol. 7:10354
  PMID: 26838601
• Foulke-Abel et al. (2016) Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Nat Commun. 150:638
  PMID: 26677983
• Mou et al. (2016) Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells Cell: Stem Cell. 19:217
  PMID: 27320041
• Zhang et al. (2016) Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation. Cell Stem Cell. 18:653
  PMID: 27133794
• Lu et al. (2016) Generation of serotonin neurons from human pluripotent stem cells. Nat Biotechnol. 34:89
  PMID: 26655496
• Huang et al. (2016) Generating trunk neural crest from human pluripotent stem cells. PLoS One. 6:19727
  PMID: 26812940
• Capowski et al. (2016) Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells. Stem Cells. 34:2625
  PMID: 27301076
• Chesnokova et al. (2016) GH is permissive for neoplastic colon growth. Proc Natl Acad Sci U S A. 113:E3250
  PMID: 27226307
• Nakaoka et al. (2016) Allelic Imbalance in Regulation of ANRIL through Chromatin Interaction at 9p21 Endometriosis Risk Locus. PLoS Genet. 12:e1005893
  PMID: 27055116
• Fattahi et al. (2016) Deriving human ENS lineages for cell therapy and drug discovery in Hirschsprung disease. Nature. 531:105
  PMID: 26863197
• Lin et al. (2016) HP Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem Cells Transl Med. 2016;
  PMID: 27591141
• Leung et al. (2016) WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate. Development. 143:398
  PMID: 26839343
• Kearns et al. (2015) Functional annotation of native enhancers with a Cas9-histone demethylase fusion. Nat Methods. 12:401
  PMID: 25775043
• Nostro et al. (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. Eur J Immunol. 4:591
  PMID: 25843049
• Zhang et al. (2015) GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis. PLoS Biol. 13:e1002129
  PMID: 25860027
• Francipane and Lagasse (2015) The lymph node as a new site for kidney organogenesis. Stem Cells Transl Med. 4:295
  PMID: 25646529
• Watmuff et al. (2015) Human pluripotent stem cell derived midbrain PITX3(eGFP/w) neurons: a versatile tool for pharmacological screening and neurodegenerative modeling. Front Cell Neurosci. 9:104
  PMID: 25873861
• Jain et al. (2015) Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung. Nat Commun. 6:6727
  PMID: 25865356
• Huebsch et al. (2015) Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales. Tissue Eng Part C Methods. 21:467
  PMID: 25333967
• Hugo et al. (2015) Non-genomic and Immune Evolution of Melanoma Acquiring MAPKi Resistance. Cell. 162:1271
  PMID: 26359985
• Zhang et al. (2015) Small Molecules Efficiently Reprogram Human Astroglial Cells into Functional Neurons. Cell Stem Cell. 17:735
  PMID: 26481520
• Morizane et al. (2015) Nephron organoids derived from human pluripotent stem cells model kidney development and injury. Stem Cell Reports. 33:1193
  PMID: 26458176
• Ooi et al. (2015) Inhibition of Excessive Monoamine Oxidase A/B Activity Protects Against Stress-induced Neuronal Death in Huntington Disease. Nat Chem Biol. 52:1850
  PMID: 25398695
• Hill et al. (2015) Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter. Mol Neurobiol. 45:1103
  PMID: 25627813
• Du et al. (2015) Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells. Nat.Commun.. 6:6626
  PMID: 25806427
• Thorne et al. (2015) GSK-3 modulates cellular responses to a broad spectrum of kinase inhibitors. Mol Biol Cell. 11:58
  PMID: 25402767
• Barbosa et al. (2015) Phosphorylation and SCF-mediated degradation regulate CREB-H transcription of metabolic targets. Stem Cell Reports. 26:2939
  PMID: 26108621
• Mutze et al. (2015) Enolase 1 (ENO1) and protein disulfide-isomerase associated 3 (PDIA3) regulate Wnt/β-catenin-driven trans-differentiation of murine alveolar epithelial cells. Cell Rep. 8:877
  PMID: 26035385
• Srikanth et al. (2015) Genomic DISC1 Disruption in hiPSCs Alters Wnt Signaling and Neural Cell Fate. Sci Rep. 12:1414
  PMID: 26299970
• Solari et al. (2015) Manganese Superoxide Dismutase Gene Expression Is Induced by Nanog and Oct4, Essential Pluripotent Stem Cells' Transcription Factors. Mol Syst Biol. 10:e0144336
  PMID: 26642061
• Sladitschek and Neveu (2015) The bimodally expressed microRNA miR-142 gates exit from pluripotency. J Biol Chem. 11:850
  PMID: 26690966
• Orlova et al. (2014) Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts. Arterioscler Thromb Vasc Biol. 34:177
  PMID: 24158517
• Shelton et al. (2014) Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells. Dis Model Mech. 3:516
  PMID: 25241748
• Zhu et al. (2014) Mouse liver repopulation with hepatocytes generated from human fibroblasts. Nature. 508:93
  PMID: 24572354
• Wu et al. (2014) A novel suppressive effect of alcohol dehydrogenase 5 in neuronal differentiation. Stem Cell Res. 289:20193
  PMID: 24895131
• Muthusamy et al. (2014) A method to identify and isolate pluripotent human stem cells and mouse epiblast stem cells using lipid body-associated retinyl ester fluorescence. Stem Cell Reports. 3:169
  PMID: 25068130
• Skelton et al. (2014) SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development. J Cachexia Sarcopenia Muscle. 13:172
  PMID: 24968096
• Naujok et al. (2014) Cytotoxicity and activation of the Wnt/beta-catenin pathway in mouse embryonic stem cells treated with four GSK3 inhibitors. BMC Res Notes. 7:273
  PMID: 24779365

There are no citations that match your criteria.

Customer Images

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• (13)

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• ESC culture

  Name: Xiaoyu Zhang
  Species: Mouse
  Assay Type: In Vitro
  Cell Line/Tissue: ESC
  In vitro cell culture addition. The image shows the morphology of ESC colonies after conversion using the treatment condition. This condition supported the conversion and long-term maintenance of dome-shaped ESCs from primed ESCs.
  

• Differentiation to cartilage

  Name: Julieta O'Flaherty
  Species: Human
  Assay Type: In Vitro
  Cell Line/Tissue: iPSCs
  I used at 3uM to generate cartilage from iPSCs, worked really well. Keep at -80C for long term storage
  

• Kidney organoids

  Name: Kirsty Rooney
  Species: Human
  Assay Type: In Vitro
  Cell Line/Tissue: MAN13 hESCs
  I use 8uM of CHIR to differentiate hESCs to intermediate mesoderm and then to kidney organoids
  

• Differentiation of human pluripotent stem cells

  Name: Paul Humphreys
  Species: Human
  Assay Type: In Vitro
  Cell Line/Tissue: hPSCs
  Directed differentiation of human pluripotent stem cells (hPSCs) towards mesoderm lineages. Used at concentration of 3uM. Very effective in induction of mesoderm markers.
  Perform titration against your cell line to optimise correct concentration.
  

• iPSC differentiation

  Name: Stephanie Hsiao
  Species: Human
  Assay Type: In Vitro
  Cell Line/Tissue: iPSC
  Differentiation of human iPSCs into hematopoietic progenitor cells.
  iPSC culture medium supplemented with 4 uM of CHIR99021.
  

• It worked good

  Name: Anonymous
  Species: Mouse
  Assay Type: In Vitro
  Cell Line/Tissue: iPSC
  Reprogramming media comprises of 3 μM CHIR99021 and 0.5 μM A-83-01.
  The cells were then maintained in RM 15 days with fresh media every other day
  PMID: 29681516
  

• For maintenance of stem cells pluripotency

  Name: Anonymous
  Species: Mouse
  Assay Type: In Vitro
  Cell Line/Tissue: Neural Stem Cells
  For directed differentiation to neural, cells are treated with GSK-3 inhibitor, CHIR99021 to induce canonical WNT signaling. This product gave me reproducible results.
  It's better to aliquot and keep it at -80°C for long term storage.
  PMID: 27133794
  

• Works as intended.

  Name: Anonymous
  Species: Rat
  Assay Type: In Vitro
  Cell Line/Tissue: Sprague-Dawley rats primary neuronal
  10 μM for 2 h
  PMID: 31291571 Reference
  

• Differentiation of human dopaminergic neurons from stem cells

  Name: Anonymous
  Species: Human
  Assay Type: In Vitro
  Cell Line/Tissue: Stem Cells
  Used this product routinely to differentiate dopaminergic neurons from human stem cells.
  

• The hiPSCs were maintained with Essential 8 Flex Medium with CHIR 99021

  Name: Anonymous
  Species: Human
  Assay Type: In Vitro
  Cell Line/Tissue: ipsc
  1.4 μM
  PMID: 29939162
  

• All iPSC lines and R1 mouse ES cells were maintained on feeders in KO-DMEM (Invitrogen) with 3 mM CHIR99021, and 1 mM PD0325901.

  Name: Anonymous
  Species: Mouse
  Assay Type: In Vitro
  Cell Line/Tissue: ipsc
  All iPSC lines and R1 mouse ES cells were maintained on feeders in KO-DMEM (Invitrogen) with 3 mM CHIR99021, and 1 mM PD0325901.
  PMID: 29358044
  

• works great for Wnt activation

  Name: avinash waghray
  Species: Human
  Assay Type: In Vitro
  In Tissue culture for growing basal cells
  PMID: 27320041

• Check the maintenance of naive pluripotency in 2i

  Name: Megha PB
  Species: Mouse
  Assay Type: In Vitro
  Cell Line/Tissue: E14TG2a
  Culture mouse ES cells in 2i media and without 2i to assess differentiation. Only the cells in 2i are pluripotent while mES cells cultured in -2i for 48 hours exit naive pluripotency.
  

• reliable product

  Name: Pinyuan Tian
  Species: Human
  Assay Type: In Vitro
  Cell Line/Tissue: pluripotent stem cells
  For directed differentiation of pluripotent stem cells to kidney organoids, cells are treated with GSK-3 inhibitor, CHIR99021 to induce canonical WNT signaling. CHIR99021 concentration and differentiation time period are the key factors for this protocol. I found this product gave me reproducible results.
  Although the datasheet says store at -20°C, it's better to aliquot and keep it at -80°C for long term storage.
  

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