Tranylcypromine hydrochloride

Name: Tranylcypromine hydrochloride
Brand: Tocris Bioscience
SKU: 3852
Rating: 5 (2 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 3852

Citations (8)

(2)

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Key Product Details

Description:	Irreversible inhibitor of LSD1; also inhibits MAO
Alternative Names:	2-PCPA
Chemical Name:	(±)-trans-2-Phenylcyclopropylamine hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	169.65

View Full Technical Details for Tranylcypromine hydrochloride

Biological Activity for Tranylcypromine hydrochloride

Tranylcypromine hydrochloride is an irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) and monoamine oxidase (MAO). Inhibits histone demethylation. In combination with CHIR 99021 (Cat. No. 4423), enables reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4, into induced pluripotent stem (iPS) cells.

For more information about how Tranylcypromine hydrochloride may be used, see our protocol: Highly Efficient Generation of CiPSCs from MEFs

Compound Libraries for Tranylcypromine hydrochloride

Tranylcypromine hydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen FDA-Approved Drugs and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Tranylcypromine hydrochloride

M. Wt	169.65
Formula	C₉H₁₁N.HCl
Storage	Desiccate at RT
Purity	≥98% (HPLC)
CAS Number	1986-47-6
PubChem ID	2723716
InChi Key	ZPEFMSTTZXJOTM-OULXEKPRSA-N
Smiles	N[C@H]1[C@H]([C@]2=CC=CC=C2)C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Tranylcypromine hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	16.96	100
	DMSO	16.96	100

Preparing Stock Solutions for Tranylcypromine hydrochloride

The following data is based on the product molecular weight 169.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.89 mL	29.47 mL	58.94 mL
5 mM	1.18 mL	5.89 mL	11.79 mL
10 mM	0.59 mL	2.95 mL	5.89 mL
50 mM	0.12 mL	0.59 mL	1.18 mL

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References for Tranylcypromine hydrochloride

References are publications that support the biological activity of the product.

Li Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. Stem Cells 2009 PMID: 19839055
Schmidt and McCafferty trans-2-Phenylcyclopropylamine is a mechanism-based inactivator of the histone demethylase LSD1. Biochemistry 2007 PMID: 17367163
Lee Histone H3 lysine 4 demethylation is a target of nonselective antidepressive medications. Chem.Biol. 2006 PMID: 16793513

Citations for Tranylcypromine hydrochloride (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Tranylcypromine hydrochloride include:

Liu et al. (2018) CRISPR-based chromatin remodeling of the endogenous Oct4 or Sox2 locus enables reprogramming to pluripotency. Cell Stem Cell. 22:252
PMID: 29358044
Sun (2018) Histone demethylase LSD1 regulates bone mass by controlling WNT7B and BMP2 signaling in osteoblasts. Bone?Res. 6:14
PMID: 29707403
Li et al. (2018) Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation. Nat Commun. 9:1420
PMID: 29650963
Sheng et al. (2018) A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation. Nat Commun. 9:4047
PMID: 30279449
Ping et al. (2018) Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs. Cell Death Dis. 9:187
PMID: 29416007
Zhang et al. (2016) Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation. Cell Stem Cell. 18:653
PMID: 27133794
Kearns et al. (2015) Functional annotation of native enhancers with a Cas9-histone demethylase fusion. Nat Methods. 12:401
PMID: 25775043
Li et al. (2011) Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules. Cell. 21:196
PMID: 20956998

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Customer Reviews for Tranylcypromine hydrochloride (2)

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We got expected effect on cells

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: Neural Stem Cells

Cells are treated with 2 µM Tranylcypromine hydrochloride.

PMID: 27133794
To start reprogramming, cultures were switched to reprogramming medium (ES medium supplemented with 10 mM Parnate)

Name: Peng Liu

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: ips

To start reprogramming, cultures were switched to reprogramming medium (ES medium supplemented with 10 mM Parnate, 3 mM Chir99021, 1 mM A83-01, and 10 mM Forskolin)

PMID: 29358044

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Product Documents for Tranylcypromine hydrochloride

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.