Human Troponin T (Cardiac) Antibody

Name: Human Troponin T (Cardiac) Antibody
Brand: R&D Systems
SKU: MAB1874
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1874

Citations (9)

(3)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Human cardiac Troponin T
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 200805
Specificity:	Detects purified human Troponin T (Cardiac) in direct ELISAs.

View Full Technical Details for Human Troponin T (Cardiac) Antibody

Applications for Human Troponin T (Cardiac) Antibody

	Recommended Concentration	Sample
Immunohistochemistry	8-25 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Troponin T (Cardiac) Antibody

	Immunocytochemistry Troponin T in BG01V Human Embryonic Stem Cells. Troponin T was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
	Immunohistochemistry Troponin T in Human Heart. Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Troponin T

Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.

Item	Value
Entrez Gene IDs	7139 (Human)
Alternate Names	CMD1D, CMH2, CMPD2, Cardiac Muscle Troponin T, Cardiomyopathy, Dilated 1D (Autosomal Dominant), Cardiomyopathy, Hypertrophic, LVNC6, RCM3, TNNT2, TnTC, Troponin T, Troponin T2, Cardiac Type, Truncated Cardiac Troponin T, cTnT, troponin T type 2 (cardiac), troponin T, cardiac muscle

Citations for Human Troponin T (Cardiac) Antibody (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Troponin T (Cardiac) Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

R Pivato et al. (2022), hESC derived cardiomyocyte biosensor to detect the different types of arrhythmogenic properties of drugs Oncogene, 1216(0):339959.
PMID: 35691674
Species: Human
Sample Types: Whole Cells
Applications: ICC

YH Chen et al. (2022), Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1 Oncogene, 63(0):102845.
PMID: 35728441
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Jung et al. (2021), Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease Stem Cell Research, 53(0):102321.
PMID: 33878708
Species: Human
Sample Types: Whole Cells
Applications: ICC

PS Huang et al. (2021), Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1 Stem Cell Research, 54(0):102415.
PMID: 34118566
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

F Pei et al. (2017), Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells Stem Cell Res, 19(0):94-103.
PMID: 28110125
Species: Rat
Sample Types: Whole Cells
Applications: ICC

X Zhang et al. (2017), Differentiation and characterization of rhesus monkey atrial and ventricular cardiomyocytes from induced pluripotent stem cells Stem Cell Res, 20(0):21-29.
PMID: 28249229
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Zhang Q et al. (2011), Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals. Cell Res., 21(4):579-87.
PMID: 21102549
Species: Human
Sample Types: Whole Cells
Applications: ICC

Li TS et al. (2011), Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair. Cardiovasc. Res., 89(1):157-65.
PMID: 20675298
Species: Human
Sample Types: Whole Cells
Applications: ICC

Ilancheran S et al. (2007), Stem cells derived from human fetal membranes display multilineage differentiation potential. Biol. Reprod., 77(3):577-88.
PMID: 17494917

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Customer Reviews for Human Troponin T (Cardiac) Antibody (3)

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Human Troponin T (Cardiac) Antibody

Name: Anonymous

Application: Flow

Sample Tested: iPSC-CMs

Species: Human

Verified Customer | Posted 07/04/2018
Human Troponin T (Cardiac) Antibody

Name: Harsha Vardhan Renikunta

Application: ICC/IF

Sample Tested: Human iPSC-Derived Cardiomyocytes

Species: Human

Verified Customer | Posted 05/19/2018
Human Troponin T (Cardiac) Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Induced Pluripotent Stem Cells

Species: Human

Verified Customer | Posted 12/15/2016

We use this 'Human Troponin T (Cardiac) Antibody' for staining differentiated cardiac cells from non-muscle cells (iPSc, Fibroblasts, etc.).

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Human Troponin T (Cardiac) Antibody

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Applications for Human Troponin T (Cardiac) Antibody

Scientific Data Examples for Human Troponin T (Cardiac) Antibody

Immunocytochemistry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Troponin T

Citations for Human Troponin T (Cardiac) Antibody (9)

Customer Reviews for Human Troponin T (Cardiac) Antibody (3)

Customer Images

Human Troponin T (Cardiac) Antibody

Human Troponin T (Cardiac) Antibody

Human Troponin T (Cardiac) Antibody

Product Documents for Human Troponin T (Cardiac) Antibody

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COA

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