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Recombinant Monoclonal Antibodies

Recombinant monoclonal antibodies are antibodies that are produced using recombinant DNA technology. Conventional monoclonal antibodies are generated by immunizing animals with immunogens to provoke immune responses. B cells are isolated from the immunized animals and fused with a myeloma cell line to create immortalized hybridomas. Recombinant monoclonal antibodies, on the other hand, are produced using in vitro cloning. Genes for an antibody's light and heavy chains are inserted into expression vectors, which are then transfected into host cells for expression.

Bio-Techne offers a wide selection of recombinant monoclonal antibodies. We produce our recombinant monoclonals using a variety of methods, from expressing new antibodies to converting our existing hybridomas or rabbit antibodies to recombinant versions. By expressing our antibodies recombinantly, we can provide an immortal supply of consistently high performing antibodies.

Detection of PD-L1/B7-H1  in RAW264.7 mouse cell line by flow cytometry.
R&D Systems rabbit recombinant monoclonal antibody is used to detect PD-L1/B7-H1.
Novus Biologicals Brand Logo

Novus Biologicals® Recombinant Antibodies

Our Novus Biologicals brand offers an expanded selection of high-quality recombinant monoclonal antibodies that offer improved antigen recognition. Novus antibodies are validated in accordance with recommendations set by the International Working Group of Antibody Validation (IWGAV) and are backed by our 100% guarantee. Novus brand recombinant antibodies reduce experimental variability, thus saving you time and money.

Advantages of Recombinant Antibodies

Recombinant antibodies offer multiple advantages compared to traditional monoclonal and polyclonal antibodies.

Recombinant Antibodies are Easily Engineered/Modified

Once the peptide sequence of an antibody is known, it can be modified to fit your needs. For example, the Fc fragment of a recombinant antibody can be exchanged with another antibody class or species. In addition, the antigen-binding region can be modified to increase binding affinity or specificity. 

Recombinant Antibodies Exhibit Superior Lot-to-Lot Consistency

Recombinant antibodies exhibit higher lot-to-lot consistency compared to traditional monoclonal antibodies as they avoid the issues of genetic drift and instability, which are typically associated with classical hybridoma-based technology.

Recombinant Antibodies are Quicker to Produce

Since animal immunization or hybridoma optimization is not needed, recombinant antibodies can be produced within weeks as opposed to months.

Recombinant Antibody Production is Scalable

The large-scale production of antibodies is typically needed for the development of diagnostic assays and new therapeutic agents. Because recombinant antibodies are produced by in vitro methods, it is easy to scale up the production of these reagents, even into the gram quantities upon request. Additionally, since we develop our recombinant antibodies in-house, we can provide our customers with a continual supply.

Recombinant Antibody Manufacturing is Animal-Free

Unlike traditional antibodies, production of recombinant antibodies does not require the use of animals. They are purified from the tissue culture supernatant of transfected host cell lines.

Additional Products

Bio-Techne offers llamabodies, also called camelid antibodies and nanobodies, for SARS-CoV-2 research.

LlaMABody Products

Learn more about our LlaMABody product line from our R&D Systems brand and how these recombinant camelid antibodies can help you detect hard-to-reach epitopes.

Spacially mapped expression

RNAscope ISH Technology

Learn more about RNAscope in situ hybridization (ISH) technology from our ACD brand and how it provides gene expression information within the spatial context of the tissue.

Bio-Techne, through its Tocris brand, offers agonists, antagonists, and other small molecules for a range of research areas.

Tocris® Small Molecules and Peptides

Through the Tocris brand, we offer an extended range of small molecules and peptides for biomedical research including agonists and antagonists, ion channel modulators, and enzyme inhibitors.

Additional Resources for Recombinant Antibodies

R&D Systems wall poster outlining our process of converting an existing hybridoma-derived antibody into a recombinant monoclonal antibody.

Recombinant Antibody Engineering Poster

Download this poster from the R&D Systems site to learn about the advantages of our antibody engineering services and how we can customize your next project.

R&D Systems application note providing evidence that our converted recombinant monoclonal antibodies bioequivalent to their original antibodies.

Recombinant Antibody Cloning Application Note

Download this application note to view performance data that shows recombinant antibodies converted from our traditional antibodies are bioequivalent to the original antibody.

5 pillar antibody validation teaser

Antibody Validation

Bio-Techne has always been at the forefront of antibody validation testing. Following our established validation procedures, as well as the 5 Pillars of Antibody Validation, our highly tested antibodies generate results you can trust.