trans-ISRIB

Product Description

trans-ISRIB is a potent integrated stress response (ISR) inhibitor; reverses the effects of eIF2α phosphorylation (IC₅₀ = 5 nM) and restores cell translation capacity. Acts downstream of PERK, targeting interactions between eIF2α kinases and elF2B. Blocks ATF4 production without altering the IRE1 or ATF6 responses in vitro. Displays memory enhancement in hippocampus-dependent spatial and fear-associated learning in rodents. trans-ISRIB promotes survival of pluripotent stem cells in culture when used in combination with Chroman 1 (Cat. No. 7163), Emricasan (Cat. No. 7310), and Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), a combination known as CEPT. When used as part of the CEPT cocktail, trans-ISRIB improves survival of differentiated cells following cryopreservation. Brain penetrant.

Solubility

Preparing Stock Solutions for trans-ISRIB

The following data is based on the product molecular weight 451.34

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	22.16 mL	110.78 mL	221.56 mL
0.5 mM	4.43 mL	22.16 mL	44.31 mL
1 mM	2.22 mL	11.08 mL	22.16 mL
5 mM	0.44 mL	2.22 mL	4.43 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Chen A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 2021 PMID: 33941937
• Sekine Stress responses. Mutations in a translation initiation factor identify the target of a memory-enhancing compound. Science 2015 PMID: 25858979
• Hinnebusch Cell biology. Blocking stress response for better memory? Science 2015 PMID: 26023119
• Sidrauski Pharmacological brake-release of mRNA translation enhances cognitive memory. Elife 2013 PMID: 23741617