Chroman 1

Product Description

Chroman 1 is a highly potent and selective ROCK2 (Rho-kinase) inhibitor (IC₅₀ values are 1 and 52 pM at ROCK2 and ROCK1, respectively). Chroman 1 exhibits >2000-fold selectivity for ROCK2 over a range of related kinases including MRCK, PKA, and AKT1 (IC₅₀ values are 150, >20000, and >20000 nM, respectively). Chroman 1 promotes survival of pluripotent stem cells (PSCs) in culture when used in combination with Emricasan (Cat. No. 7310), Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), and trans-ISRIB (Cat. No. 5284), a combination known as CEPT. When used as part of the CEPT cocktail, Chroman 1 also improves survival of differentiated cells following cryopreservation with cardiomyocyte cell survival increasing by 36% and motor neuron survival improving by 63% compared with DMSO controls.

Solubility

Preparing Stock Solutions for Chroman 1

The following data is based on the product molecular weight 509.43

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.96 mL	9.81 mL	19.63 mL
5 mM	0.39 mL	1.96 mL	3.93 mL
10 mM	0.20 mL	0.98 mL	1.96 mL
50 mM	0.04 mL	0.20 mL	0.39 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Chen A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 2021 PMID: 33941937
• Chen Asymmetric synthesis of potent chroman-based Rho kinase (ROCK-II) inhibitors. Med.Chem.Commun.