Polyamine Supplement x1000 (lyophilized) is a media supplement comprising a lyophilized mixture of Putrescine, Spermine (Cat. No. 0958) and Spermidine. The product is used as a component of the CEPT cocktail, in combination with Emricasan (Cat. No. 7310), Chroman 1 (Cat. No. 7163) and trans-ISRIB (Cat. No. 5284) to enhance viability of human pluripotent stem cells (hPSCs) and their differentiated progeny. Polyamine Supplement x1000 (lyophilized) also improves survival of pluripotent and differentiated cells during cryopreservation, organoid formation, single-cell cloning, and genome editing.
NOTE: This reagent is provided lyophilized. To use prepare a 1000x stock solution by reconstituting vial contents in 5 mL of ddH2O and filter sterilize before use. Add 1 mL of sterile resuspended polyamines supplement (1000x stock) per 1 L of media.
